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Genome evolution during bread wheat formation unveiled by the distribution dynamics of SSR sequences on chromosomes using FISH.
BMC Genomics. 2021 Jan 14; 22(1):55.BG

Abstract

BACKGROUND

During the bread wheat speciation by polyploidization, a series of genome rearrangement and sequence recombination occurred. Simple sequence repeat (SSR) sequences, predominately located in heterochromatic regions of chromosomes, are the effective marker for tracing the genomic DNA sequence variations. However, to date the distribution dynamics of SSRs on chromosomes of bread wheat and its donors, including diploid and tetraploid Triticum urartu, Aegilops speltoides, Aegilops tauschii, Triticum turgidum ssp. dicocoides, reflecting the genome evolution events during bread wheat formation had not been comprehensively investigated.

RESULTS

The genome evolution was studied by comprehensively comparing the distribution patterns of (AAC)n, (AAG)n, (AGC)n and (AG)n in bread wheat Triticum aestivum var. Chinese Spring and its progenitors T. urartu, A. speltoides, Ae. tauschii, wild tetroploid emmer wheat T. dicocoides, and cultivated emmer wheat T. dicoccum. Results indicated that there are specific distribution patterns in different chromosomes from different species for each SSRs. They provided efficient visible markers for identification of some individual chromosomes and SSR sequence evolution tracing from the diploid progenitors to hexaploid wheat. During wheat speciation, the SSR sequence expansion occurred predominately in the centromeric and pericentromeric regions of B genome chromosomes accompanied by little expansion and elimination on other chromosomes. This result indicated that the B genome might be more sensitive to the "genome shock" and more changeable during wheat polyplodization.

CONCLUSIONS

During the bread wheat evolution, SSRs including (AAC)n, (AAG)n, (AGC)n and (AG)n in B genome displayed the greatest changes (sequence expansion) especially in centromeric and pericentromeric regions during the polyploidization from Ae. speltoides S genome, the most likely donor of B genome. This work would enable a better understanding of the wheat genome formation and evolution and reinforce the viewpoint that B genome was originated from S genome.

Authors+Show Affiliations

State Key Laboratory of Plant Cell and Chromosome Engineering, Institute of Genetics and Developmental Biology, Innovation Academy for Seed Design, Chinese Academy of Sciences, Beijing, 100101, China. College of Agriculture, Yangtze University, Jingzhou, 434000, Hubei, China.State Key Laboratory of Plant Cell and Chromosome Engineering, Institute of Genetics and Developmental Biology, Innovation Academy for Seed Design, Chinese Academy of Sciences, Beijing, 100101, China. cmfan@genetics.ac.cn.State Key Laboratory of Plant Cell and Chromosome Engineering, Institute of Genetics and Developmental Biology, Innovation Academy for Seed Design, Chinese Academy of Sciences, Beijing, 100101, China.United States Department of Agriculture, Agricultural Research Service, Forage and Range Research Laboratory, Utah State University, Logan, UT, 84322-6300, USA.State Key Laboratory of Plant Cell and Chromosome Engineering, Institute of Genetics and Developmental Biology, Innovation Academy for Seed Design, Chinese Academy of Sciences, Beijing, 100101, China.State Key Laboratory of Plant Cell and Chromosome Engineering, Institute of Genetics and Developmental Biology, Innovation Academy for Seed Design, Chinese Academy of Sciences, Beijing, 100101, China.State Key Laboratory of Plant Cell and Chromosome Engineering, Institute of Genetics and Developmental Biology, Innovation Academy for Seed Design, Chinese Academy of Sciences, Beijing, 100101, China. zmhu@genetics.ac.cn. College of Agriculture, University of Chinese Academy of Sciences, Beijing, 100049, China. zmhu@genetics.ac.cn.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

33446108

Citation

Zhang, Yingxin, et al. "Genome Evolution During Bread Wheat Formation Unveiled By the Distribution Dynamics of SSR Sequences On Chromosomes Using FISH." BMC Genomics, vol. 22, no. 1, 2021, p. 55.
Zhang Y, Fan C, Chen Y, et al. Genome evolution during bread wheat formation unveiled by the distribution dynamics of SSR sequences on chromosomes using FISH. BMC Genomics. 2021;22(1):55.
Zhang, Y., Fan, C., Chen, Y., Wang, R. R., Zhang, X., Han, F., & Hu, Z. (2021). Genome evolution during bread wheat formation unveiled by the distribution dynamics of SSR sequences on chromosomes using FISH. BMC Genomics, 22(1), 55. https://doi.org/10.1186/s12864-020-07364-6
Zhang Y, et al. Genome Evolution During Bread Wheat Formation Unveiled By the Distribution Dynamics of SSR Sequences On Chromosomes Using FISH. BMC Genomics. 2021 Jan 14;22(1):55. PubMed PMID: 33446108.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Genome evolution during bread wheat formation unveiled by the distribution dynamics of SSR sequences on chromosomes using FISH. AU - Zhang,Yingxin, AU - Fan,Chengming, AU - Chen,Yuhong, AU - Wang,Richard R-C, AU - Zhang,Xiangqi, AU - Han,Fangpu, AU - Hu,Zanmin, Y1 - 2021/01/14/ PY - 2020/08/20/received PY - 2020/12/30/accepted PY - 2021/1/15/entrez PY - 2021/1/16/pubmed PY - 2021/5/15/medline KW - Bread wheat KW - FISH KW - Polyploidization KW - Simple sequence repeat SP - 55 EP - 55 JF - BMC genomics JO - BMC Genomics VL - 22 IS - 1 N2 - BACKGROUND: During the bread wheat speciation by polyploidization, a series of genome rearrangement and sequence recombination occurred. Simple sequence repeat (SSR) sequences, predominately located in heterochromatic regions of chromosomes, are the effective marker for tracing the genomic DNA sequence variations. However, to date the distribution dynamics of SSRs on chromosomes of bread wheat and its donors, including diploid and tetraploid Triticum urartu, Aegilops speltoides, Aegilops tauschii, Triticum turgidum ssp. dicocoides, reflecting the genome evolution events during bread wheat formation had not been comprehensively investigated. RESULTS: The genome evolution was studied by comprehensively comparing the distribution patterns of (AAC)n, (AAG)n, (AGC)n and (AG)n in bread wheat Triticum aestivum var. Chinese Spring and its progenitors T. urartu, A. speltoides, Ae. tauschii, wild tetroploid emmer wheat T. dicocoides, and cultivated emmer wheat T. dicoccum. Results indicated that there are specific distribution patterns in different chromosomes from different species for each SSRs. They provided efficient visible markers for identification of some individual chromosomes and SSR sequence evolution tracing from the diploid progenitors to hexaploid wheat. During wheat speciation, the SSR sequence expansion occurred predominately in the centromeric and pericentromeric regions of B genome chromosomes accompanied by little expansion and elimination on other chromosomes. This result indicated that the B genome might be more sensitive to the "genome shock" and more changeable during wheat polyplodization. CONCLUSIONS: During the bread wheat evolution, SSRs including (AAC)n, (AAG)n, (AGC)n and (AG)n in B genome displayed the greatest changes (sequence expansion) especially in centromeric and pericentromeric regions during the polyploidization from Ae. speltoides S genome, the most likely donor of B genome. This work would enable a better understanding of the wheat genome formation and evolution and reinforce the viewpoint that B genome was originated from S genome. SN - 1471-2164 UR - https://www.unboundmedicine.com/medline/citation/33446108/Genome_evolution_during_bread_wheat_formation_unveiled_by_the_distribution_dynamics_of_SSR_sequences_on_chromosomes_using_FISH_ L2 - https://bmcgenomics.biomedcentral.com/articles/10.1186/s12864-020-07364-6 DB - PRIME DP - Unbound Medicine ER -