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Phytosynthesis of Silver Nanoparticles Using Perilla frutescens Leaf Extract: Characterization and Evaluation of Antibacterial, Antioxidant, and Anticancer Activities.
Int J Nanomedicine. 2021; 16:15-29.IJ

Abstract

Purpose

The present study investigates the phytosynthesis of silver nanoparticles (AgNPs) using Perilla frutescens leaf extract, which acts as a reducing agent for the conversion of silver ions (Ag+) into AgNPs. P. frutescens leaf synthesized AgNPs (PF@AgNPs) were evaluated for biomedical properties including antibacterial, antioxidant and anticancer activities.

Materials and Methods

PF@AgNPs were synthesized using P. frutescens leaf extract and silver nitrate solution. The morphology and physical properties of PF@AgNPs were studied by spectroscopic techniques including, UV-Vis, FTIR, TEM, XRD, DLS, and TGA. Antibacterial activity of PF@AgNPs was evaluated by disk diffusion assay. Antioxidant activity of PF@AgNPs was checked by 2.2-diphenyl-1-picrylhydrazyl (DPPH), and 2.2'-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) free radical scavenging assays. Anticancer activity of PF@AgNPs was checked by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide assay. Cytotoxic effects of PF@AgNPs on most susceptible cancer cell lines were observed by phase contrast microscopy.

Results

PF@AgNPs showed surface plasmon resonance peak at 461 nm. XRD pattern showed that the PF@AgNPs were face-centered cubic crystals with a mean size of 25.71 nm. TEM analysis revealed the different shapes (spherical, rhombic, triangle, and rod) of PF@AgNPs. Zeta potential value (-25.83 mV) indicated that PF@AgNPs were long-term stable and not agglomerated. A low polydispersity index value (0.389) indicated the monodispersity of PF@AgNPs. TGA revealed the high thermal stability of PF@AgNPs. PF@AgNPs exhibited maximum inhibition against Escherichia coli, followed by Bacillus subtilis and Staphylococcus aureus. PF@AgNPs showed maximum inhibition of 68.02 and 62.93% against DPPH and ABTS-free radicals, respectively. PF@AgNPs showed significant anticancer activity against human colon cancer (COLO205) and prostate adenocarcinoma (LNCaP). PF@AgNPs exhibited apoptotic effects on LNCaP cells including cell shrinkage, membrane blebbing, chromatin condensation, fragmentation of nuclei, and formation of apoptotic bodies.

Conclusion

The present study reports the successful synthesis of PF@AgNPs using P. frutescens leaf extract. The synthesized PF@AgNPs are FCC crystals, monodispersed, long-term stable, and non-agglomerated. The observed antibacterial, antioxidant, and anticancer activities demonstrate the potential biomedical applications of PF@AgNPs.

Authors+Show Affiliations

School of Chemical Engineering and Technology, North University of China, Taiyuan, Shanxi Province, People's Republic of China.School of Chemical Engineering and Technology, North University of China, Taiyuan, Shanxi Province, People's Republic of China.School of Chemical Engineering and Technology, North University of China, Taiyuan, Shanxi Province, People's Republic of China.Pharmacology and Toxicology Division, Indian Institute of Chemical Engineering and Technology, Hyderabad, Telangana State, India.School of Chemical Engineering and Technology, North University of China, Taiyuan, Shanxi Province, People's Republic of China.School of Chemical Engineering and Technology, North University of China, Taiyuan, Shanxi Province, People's Republic of China.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

33447027

Citation

Reddy, N V., et al. "Phytosynthesis of Silver Nanoparticles Using Perilla Frutescens Leaf Extract: Characterization and Evaluation of Antibacterial, Antioxidant, and Anticancer Activities." International Journal of Nanomedicine, vol. 16, 2021, pp. 15-29.
Reddy NV, Li H, Hou T, et al. Phytosynthesis of Silver Nanoparticles Using Perilla frutescens Leaf Extract: Characterization and Evaluation of Antibacterial, Antioxidant, and Anticancer Activities. Int J Nanomedicine. 2021;16:15-29.
Reddy, N. V., Li, H., Hou, T., Bethu, M. S., Ren, Z., & Zhang, Z. (2021). Phytosynthesis of Silver Nanoparticles Using Perilla frutescens Leaf Extract: Characterization and Evaluation of Antibacterial, Antioxidant, and Anticancer Activities. International Journal of Nanomedicine, 16, 15-29. https://doi.org/10.2147/IJN.S265003
Reddy NV, et al. Phytosynthesis of Silver Nanoparticles Using Perilla Frutescens Leaf Extract: Characterization and Evaluation of Antibacterial, Antioxidant, and Anticancer Activities. Int J Nanomedicine. 2021;16:15-29. PubMed PMID: 33447027.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Phytosynthesis of Silver Nanoparticles Using Perilla frutescens Leaf Extract: Characterization and Evaluation of Antibacterial, Antioxidant, and Anticancer Activities. AU - Reddy,N V, AU - Li,Huizhen, AU - Hou,Tianyu, AU - Bethu,M S, AU - Ren,Zhiqing, AU - Zhang,Zhijun, Y1 - 2021/01/06/ PY - 2020/05/28/received PY - 2020/09/28/accepted PY - 2021/1/15/entrez PY - 2021/1/16/pubmed PY - 2021/1/30/medline KW - LNCaP KW - Perilla frutescens KW - antibacterial activity KW - anticancer activity KW - antioxidant activity KW - silver nanoparticles SP - 15 EP - 29 JF - International journal of nanomedicine JO - Int J Nanomedicine VL - 16 N2 - Purpose: The present study investigates the phytosynthesis of silver nanoparticles (AgNPs) using Perilla frutescens leaf extract, which acts as a reducing agent for the conversion of silver ions (Ag+) into AgNPs. P. frutescens leaf synthesized AgNPs (PF@AgNPs) were evaluated for biomedical properties including antibacterial, antioxidant and anticancer activities. Materials and Methods: PF@AgNPs were synthesized using P. frutescens leaf extract and silver nitrate solution. The morphology and physical properties of PF@AgNPs were studied by spectroscopic techniques including, UV-Vis, FTIR, TEM, XRD, DLS, and TGA. Antibacterial activity of PF@AgNPs was evaluated by disk diffusion assay. Antioxidant activity of PF@AgNPs was checked by 2.2-diphenyl-1-picrylhydrazyl (DPPH), and 2.2'-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) free radical scavenging assays. Anticancer activity of PF@AgNPs was checked by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide assay. Cytotoxic effects of PF@AgNPs on most susceptible cancer cell lines were observed by phase contrast microscopy. Results: PF@AgNPs showed surface plasmon resonance peak at 461 nm. XRD pattern showed that the PF@AgNPs were face-centered cubic crystals with a mean size of 25.71 nm. TEM analysis revealed the different shapes (spherical, rhombic, triangle, and rod) of PF@AgNPs. Zeta potential value (-25.83 mV) indicated that PF@AgNPs were long-term stable and not agglomerated. A low polydispersity index value (0.389) indicated the monodispersity of PF@AgNPs. TGA revealed the high thermal stability of PF@AgNPs. PF@AgNPs exhibited maximum inhibition against Escherichia coli, followed by Bacillus subtilis and Staphylococcus aureus. PF@AgNPs showed maximum inhibition of 68.02 and 62.93% against DPPH and ABTS-free radicals, respectively. PF@AgNPs showed significant anticancer activity against human colon cancer (COLO205) and prostate adenocarcinoma (LNCaP). PF@AgNPs exhibited apoptotic effects on LNCaP cells including cell shrinkage, membrane blebbing, chromatin condensation, fragmentation of nuclei, and formation of apoptotic bodies. Conclusion: The present study reports the successful synthesis of PF@AgNPs using P. frutescens leaf extract. The synthesized PF@AgNPs are FCC crystals, monodispersed, long-term stable, and non-agglomerated. The observed antibacterial, antioxidant, and anticancer activities demonstrate the potential biomedical applications of PF@AgNPs. SN - 1178-2013 UR - https://www.unboundmedicine.com/medline/citation/33447027/Phytosynthesis_of_Silver_Nanoparticles_Using_Perilla_frutescens_Leaf_Extract:_Characterization_and_Evaluation_of_Antibacterial_Antioxidant_and_Anticancer_Activities_ L2 - https://dx.doi.org/10.2147/IJN.S265003 DB - PRIME DP - Unbound Medicine ER -