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Hydrogen sulfide is synthesized endogenously in both retinal artery and retina mostly via CSE.
Exp Eye Res. 2021 03; 204:108443.EE

Abstract

Hydrogen sulfide (H2S) is an important gasotransmitter expressed in various tissues of the organism, including the eye. It is known that H2S is localized especially in the retina and corneal layers in bovine eye. The enzymes that mediate H2S synthesis are 3-mercaptopyruvate sulfurtransferase (3-MST), cystathionine β-synthase (CBS) and cystathionine γ-lyase (CSE). Herein, we aimed to investigate the concentration levels and distribution profiles of these enzymes in bovine retina and retinal artery. Enzyme levels were measured by ELISA and distribution were determined by immunofluorescence microscopic analysis. Much higher concentrations of CBS and CSE have been detected in the retinal artery compared to the retina. In both tissues, particulary 3-MST was found at the lowest level while, CSE was determined to be the most abundant enzyme among the others. CBS distribution was shown in both endothelial and smooth muscle layers, while CSE was seen especially in the endothelial layer of the retinal artery. In the retina, CBS and CSE were expressed in cone-basil cells and retinal ganglion cells, while CSE was also present in bipolar cells. Our results indicated that H2S is synthesized endogenously in ocular tissues. The widespread expression of H2S synthesizing enzymes in the retina and retinal artery of the bovine eye, which has anatomical similarities with the human eye, may suggest a protective role for H2S against retinal vascular diseases as well as a regulatory role in the retinal vascular tone.

Authors+Show Affiliations

Department of Medical Pharmacology, Faculty of Medicine, Giresun University, Giresun, 28200, Turkey. Electronic address: selcuk_takir@yahoo.com.Department of Medical Services and Techniques, Vocational School of Health Services, Giresun University, Giresun, 28200, Turkey; Department of Pharmacology, Faculty of Pharmacy, Istanbul University, Istanbul, 34116, Turkey; Graduate School of Health Sciences, Istanbul University, Istanbul, 34116, Turkey.Department of Pharmacology, Faculty of Pharmacy, Istanbul University, Istanbul, 34116, Turkey.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

33453277

Citation

Takır, Selçuk, et al. "Hydrogen Sulfide Is Synthesized Endogenously in Both Retinal Artery and Retina Mostly Via CSE." Experimental Eye Research, vol. 204, 2021, p. 108443.
Takır S, Semiz AT, Uydeş Doğan BS. Hydrogen sulfide is synthesized endogenously in both retinal artery and retina mostly via CSE. Exp Eye Res. 2021;204:108443.
Takır, S., Semiz, A. T., & Uydeş Doğan, B. S. (2021). Hydrogen sulfide is synthesized endogenously in both retinal artery and retina mostly via CSE. Experimental Eye Research, 204, 108443. https://doi.org/10.1016/j.exer.2021.108443
Takır S, Semiz AT, Uydeş Doğan BS. Hydrogen Sulfide Is Synthesized Endogenously in Both Retinal Artery and Retina Mostly Via CSE. Exp Eye Res. 2021;204:108443. PubMed PMID: 33453277.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Hydrogen sulfide is synthesized endogenously in both retinal artery and retina mostly via CSE. AU - Takır,Selçuk, AU - Semiz,Ayça Toprak, AU - Uydeş Doğan,B Sönmez, Y1 - 2021/01/14/ PY - 2020/08/25/received PY - 2020/12/30/revised PY - 2021/01/08/accepted PY - 2021/1/17/pubmed PY - 2021/8/28/medline PY - 2021/1/16/entrez KW - 3-MST KW - CBS KW - CSE KW - Hydrogen sulfide KW - Retina KW - Retinal artery SP - 108443 EP - 108443 JF - Experimental eye research JO - Exp Eye Res VL - 204 N2 - Hydrogen sulfide (H2S) is an important gasotransmitter expressed in various tissues of the organism, including the eye. It is known that H2S is localized especially in the retina and corneal layers in bovine eye. The enzymes that mediate H2S synthesis are 3-mercaptopyruvate sulfurtransferase (3-MST), cystathionine β-synthase (CBS) and cystathionine γ-lyase (CSE). Herein, we aimed to investigate the concentration levels and distribution profiles of these enzymes in bovine retina and retinal artery. Enzyme levels were measured by ELISA and distribution were determined by immunofluorescence microscopic analysis. Much higher concentrations of CBS and CSE have been detected in the retinal artery compared to the retina. In both tissues, particulary 3-MST was found at the lowest level while, CSE was determined to be the most abundant enzyme among the others. CBS distribution was shown in both endothelial and smooth muscle layers, while CSE was seen especially in the endothelial layer of the retinal artery. In the retina, CBS and CSE were expressed in cone-basil cells and retinal ganglion cells, while CSE was also present in bipolar cells. Our results indicated that H2S is synthesized endogenously in ocular tissues. The widespread expression of H2S synthesizing enzymes in the retina and retinal artery of the bovine eye, which has anatomical similarities with the human eye, may suggest a protective role for H2S against retinal vascular diseases as well as a regulatory role in the retinal vascular tone. SN - 1096-0007 UR - https://www.unboundmedicine.com/medline/citation/33453277/Hydrogen_sulfide_is_synthesized_endogenously_in_both_retinal_artery_and_retina_mostly_via_CSE_ DB - PRIME DP - Unbound Medicine ER -