Tags

Type your tag names separated by a space and hit enter

An unattended HS-SPME-GC-MS/MS combined with a novel sample preparation strategy for the reliable quantitation of C8 volatiles in mushrooms: A sample preparation strategy to fully control the volatile emission.
Food Chem. 2021 Jun 15; 347:128998.FC

Abstract

Eight carbon (C8) compounds are the key characteristic flavors of mushrooms. The quantitative analysis of the volatiles in mushrooms is challenging especially with the unattended HS-SPME-GC-MS. An unattended HS-SPME-GC-MS/MS in combination with novel sample preparation of the complete control of volatile emissions was developed for the quantitation of the C8 volatiles in mushrooms. The sample preparation strategy was composed of freeze-drying, rehydration, and the addition of a 15% citric acid solution. With this strategy, the volatile emission from mushroom was fully controlled at a certain time point. This method was found to be highly reliable, sensitive, precise, and accurate. This method was successfully applied to measure the contents of the C8 volatiles in the beech, button, and shiitake mushrooms. 1-Octene-3-ol was the most predominant compound in the mushrooms, representing 62.4, 69.0, and 89.2% of the total C8 volatiles in the beech, button, and shiitake mushrooms, respectively.

Authors+Show Affiliations

Department of Food Science and Biotechnology, Graduate School, Woosuk University, 443 Samnye-ro, Samnye-eup, Wanju-gun, Jeonbuk Province 55338, Republic of Korea. Electronic address: munjung@woosuk.ac.kr.Department of Food Science and Biotechnology, Graduate School, Woosuk University, 443 Samnye-ro, Samnye-eup, Wanju-gun, Jeonbuk Province 55338, Republic of Korea; Institute of Jinan Red Ginseng, Jinan-gun, Jeonbuk Province, Republic of Korea.Department of Food Science and Biotechnology, Graduate School, Woosuk University, 443 Samnye-ro, Samnye-eup, Wanju-gun, Jeonbuk Province 55338, Republic of Korea.Department of Food Science and Biotechnology, Graduate School, Woosuk University, 443 Samnye-ro, Samnye-eup, Wanju-gun, Jeonbuk Province 55338, Republic of Korea.Department of Crop Science, College of Sanghuh Life Science, Kunkuk University, Seoul, Republic of Korea.National Institute of Horticultural and Herbal Science, Rural Development Administration, Eumsung, Chungbuk Province, Republic of Korea.Department of Crop Science, College of Sanghuh Life Science, Kunkuk University, Seoul, Republic of Korea.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

33453580

Citation

Jung, Mun Yhung, et al. "An Unattended HS-SPME-GC-MS/MS Combined With a Novel Sample Preparation Strategy for the Reliable Quantitation of C8 Volatiles in Mushrooms: a Sample Preparation Strategy to Fully Control the Volatile Emission." Food Chemistry, vol. 347, 2021, p. 128998.
Jung MY, Lee DE, Baek SH, et al. An unattended HS-SPME-GC-MS/MS combined with a novel sample preparation strategy for the reliable quantitation of C8 volatiles in mushrooms: A sample preparation strategy to fully control the volatile emission. Food Chem. 2021;347:128998.
Jung, M. Y., Lee, D. E., Baek, S. H., Lim, S. M., Chung, I. M., Han, J. G., & Kim, S. H. (2021). An unattended HS-SPME-GC-MS/MS combined with a novel sample preparation strategy for the reliable quantitation of C8 volatiles in mushrooms: A sample preparation strategy to fully control the volatile emission. Food Chemistry, 347, 128998. https://doi.org/10.1016/j.foodchem.2020.128998
Jung MY, et al. An Unattended HS-SPME-GC-MS/MS Combined With a Novel Sample Preparation Strategy for the Reliable Quantitation of C8 Volatiles in Mushrooms: a Sample Preparation Strategy to Fully Control the Volatile Emission. Food Chem. 2021 Jun 15;347:128998. PubMed PMID: 33453580.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - An unattended HS-SPME-GC-MS/MS combined with a novel sample preparation strategy for the reliable quantitation of C8 volatiles in mushrooms: A sample preparation strategy to fully control the volatile emission. AU - Jung,Mun Yhung, AU - Lee,Da Eun, AU - Baek,Sun Hye, AU - Lim,Su Min, AU - Chung,Ill-Min, AU - Han,Jae-Gu, AU - Kim,Seung-Hyun, Y1 - 2021/01/07/ PY - 2020/07/28/received PY - 2020/11/30/revised PY - 2020/12/30/accepted PY - 2021/1/17/pubmed PY - 2021/3/5/medline PY - 2021/1/16/entrez KW - Automation KW - Beech KW - Button KW - C8 volatiles KW - Gas chromatography KW - Headspace-solid phasemicroextraction KW - Mass spectrometry KW - Mushrooms KW - Quantitative analysis KW - Shiitake SP - 128998 EP - 128998 JF - Food chemistry JO - Food Chem VL - 347 N2 - Eight carbon (C8) compounds are the key characteristic flavors of mushrooms. The quantitative analysis of the volatiles in mushrooms is challenging especially with the unattended HS-SPME-GC-MS. An unattended HS-SPME-GC-MS/MS in combination with novel sample preparation of the complete control of volatile emissions was developed for the quantitation of the C8 volatiles in mushrooms. The sample preparation strategy was composed of freeze-drying, rehydration, and the addition of a 15% citric acid solution. With this strategy, the volatile emission from mushroom was fully controlled at a certain time point. This method was found to be highly reliable, sensitive, precise, and accurate. This method was successfully applied to measure the contents of the C8 volatiles in the beech, button, and shiitake mushrooms. 1-Octene-3-ol was the most predominant compound in the mushrooms, representing 62.4, 69.0, and 89.2% of the total C8 volatiles in the beech, button, and shiitake mushrooms, respectively. SN - 1873-7072 UR - https://www.unboundmedicine.com/medline/citation/33453580/An_unattended_HS_SPME_GC_MS/MS_combined_with_a_novel_sample_preparation_strategy_for_the_reliable_quantitation_of_C8_volatiles_in_mushrooms:_A_sample_preparation_strategy_to_fully_control_the_volatile_emission_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0308-8146(20)32889-2 DB - PRIME DP - Unbound Medicine ER -