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SARS-CoV-2, the pandemic coronavirus: Molecular and structural insights.
J Basic Microbiol. 2021 Mar; 61(3):180-202.JB

Abstract

The outbreak of a novel coronavirus associated with acute respiratory disease, called COVID-19, marked the introduction of the third spillover of an animal coronavirus (CoV) to humans in the last two decades. The genome analysis with various bioinformatics tools revealed that the causative pathogen (SARS-CoV-2) belongs to the subgenus Sarbecovirus of the genus Betacoronavirus, with highly similar genome as bat coronavirus and receptor-binding domain (RBD) of spike glycoprotein as Malayan pangolin coronavirus. Based on its genetic proximity, SARS-CoV-2 is likely to have originated from bat-derived CoV and transmitted to humans via an unknown intermediate mammalian host, probably Malayan pangolin. Further, spike protein S1/S2 cleavage site of SARS-CoV-2 has acquired polybasic furin cleavage site which is absent in bat and pangolin suggesting natural selection either in an animal host before zoonotic transfer or in humans following zoonotic transfer. In the current review, we recapitulate a preliminary opinion about the disease, origin and life cycle of SARS-CoV-2, roles of virus proteins in pathogenesis, commonalities, and differences between different corona viruses. Moreover, the crystal structures of SARS-CoV-2 proteins with unique characteristics differentiating it from other CoVs are discussed. Our review also provides comprehensive information on the molecular aspects of SARS-CoV-2 including secondary structures in the genome and protein-protein interactions which can be useful to understand the aggressive spread of the SARS-CoV-2. The mutations and the haplotypes reported in the SARS-CoV-2 genome are summarized to understand the virus evolution.

Authors+Show Affiliations

Department of Botany, Savitribai Phule Pune University, Pune, India.Department of Botany, Savitribai Phule Pune University, Pune, India.Department of Botany, Savitribai Phule Pune University, Pune, India.Department of Microbiology, Savitribai Phule Pune University, Pune, India.Department of Botany, Savitribai Phule Pune University, Pune, India.

Pub Type(s)

Journal Article
Review

Language

eng

PubMed ID

33460172

Citation

Kadam, Swapnil B., et al. "SARS-CoV-2, the Pandemic Coronavirus: Molecular and Structural Insights." Journal of Basic Microbiology, vol. 61, no. 3, 2021, pp. 180-202.
Kadam SB, Sukhramani GS, Bishnoi P, et al. SARS-CoV-2, the pandemic coronavirus: Molecular and structural insights. J Basic Microbiol. 2021;61(3):180-202.
Kadam, S. B., Sukhramani, G. S., Bishnoi, P., Pable, A. A., & Barvkar, V. T. (2021). SARS-CoV-2, the pandemic coronavirus: Molecular and structural insights. Journal of Basic Microbiology, 61(3), 180-202. https://doi.org/10.1002/jobm.202000537
Kadam SB, et al. SARS-CoV-2, the Pandemic Coronavirus: Molecular and Structural Insights. J Basic Microbiol. 2021;61(3):180-202. PubMed PMID: 33460172.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - SARS-CoV-2, the pandemic coronavirus: Molecular and structural insights. AU - Kadam,Swapnil B, AU - Sukhramani,Geetika S, AU - Bishnoi,Pratibha, AU - Pable,Anupama A, AU - Barvkar,Vitthal T, Y1 - 2021/01/18/ PY - 2020/08/29/received PY - 2020/12/22/revised PY - 2020/12/28/accepted PY - 2021/1/19/pubmed PY - 2021/3/11/medline PY - 2021/1/18/entrez KW - ACE2 KW - SARS-CoV-2 KW - angiotensin-converting enzyme 2 KW - pandemic coronavirus KW - severe acute respiratory syndrome coronavirus 2 SP - 180 EP - 202 JF - Journal of basic microbiology JO - J Basic Microbiol VL - 61 IS - 3 N2 - The outbreak of a novel coronavirus associated with acute respiratory disease, called COVID-19, marked the introduction of the third spillover of an animal coronavirus (CoV) to humans in the last two decades. The genome analysis with various bioinformatics tools revealed that the causative pathogen (SARS-CoV-2) belongs to the subgenus Sarbecovirus of the genus Betacoronavirus, with highly similar genome as bat coronavirus and receptor-binding domain (RBD) of spike glycoprotein as Malayan pangolin coronavirus. Based on its genetic proximity, SARS-CoV-2 is likely to have originated from bat-derived CoV and transmitted to humans via an unknown intermediate mammalian host, probably Malayan pangolin. Further, spike protein S1/S2 cleavage site of SARS-CoV-2 has acquired polybasic furin cleavage site which is absent in bat and pangolin suggesting natural selection either in an animal host before zoonotic transfer or in humans following zoonotic transfer. In the current review, we recapitulate a preliminary opinion about the disease, origin and life cycle of SARS-CoV-2, roles of virus proteins in pathogenesis, commonalities, and differences between different corona viruses. Moreover, the crystal structures of SARS-CoV-2 proteins with unique characteristics differentiating it from other CoVs are discussed. Our review also provides comprehensive information on the molecular aspects of SARS-CoV-2 including secondary structures in the genome and protein-protein interactions which can be useful to understand the aggressive spread of the SARS-CoV-2. The mutations and the haplotypes reported in the SARS-CoV-2 genome are summarized to understand the virus evolution. SN - 1521-4028 UR - https://www.unboundmedicine.com/medline/citation/33460172/SARS_CoV_2_the_pandemic_coronavirus:_Molecular_and_structural_insights_ L2 - https://doi.org/10.1002/jobm.202000537 DB - PRIME DP - Unbound Medicine ER -