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Evaluation of the effect of silver and silver nanoparticles on the function of selenoproteins using an in-vitro model of the fish intestine: The cell line RTgutGC.
Ecotoxicol Environ Saf. 2021 Mar 15; 211:111930.EE

Abstract

Emerging research in mammalian cells suggests that ionic (AgNO3) and nano silver (AgNP) can disrupt the metabolism of selenium which plays a vital role in oxidative stress control. However, the effect of silver (Ag) on selenoprotein function in fish is poorly understood. Here we evaluate the effects of AgNO3 and citrate coated AgNP (cit-AgNP) on selenoprotein function and oxidative stress using a fish cell line derived from the rainbow trout (Oncorhynchus mykiss) intestine (RTgutGC). Cell viability was evaluated using a cytotoxicity assay which measures simultaneously metabolic activity, membrane integrity and lysosome integrity. Cells exposed to equimolar amounts of AgNO3 and cit-AgNP accumulated the same amount of silver intracellularly, however AgNO3 was more toxic than cit-AgNP. Selenoenzymes glutathione peroxidase (GPx) and thioredoxin reductase (TrxR) mRNA levels and enzyme activity were measured. While mRNA levels remained unaffected by AgNO3 or cit-AgNP, the enzyme activity of GPx was inhibited by AgNO3 (1 µM) and cit-AgNP (5 µM) and TrxR activity was inhibited by AgNO3 (0.4 µM) and cit-AgNP (1, 5 µM). Moreover, cells exposed to 1 µM of AgNO3 and cit-AgNP showed an increase in metallothionein b (MTb) mRNA levels at 24 h of exposure, confirming the uptake of silver, but returned to control levels at 72 h suggesting silver scavenging by MTb. Oxidative stress was not observed at any of the doses of AgNO3 or cit-AgNP tested. Overall, this study shows that AgNO3 or cit-AgNP can inhibit the activity of selenoenzymes but do not induce oxidative stress in RTgutGC cells.

Authors+Show Affiliations

Department of Integrative Biology, Oklahoma State University, Stillwater, OK, USA.Department of Integrative Biology, Oklahoma State University, Stillwater, OK, USA; Department of Earth and Planetary Sciences, Washington University, Saint Louis, MO, USA.Department of Integrative Biology, Oklahoma State University, Stillwater, OK, USA.Department of Integrative Biology, Oklahoma State University, Stillwater, OK, USA. Electronic address: matteo.minghetti@okstate.edu.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

33472113

Citation

Chanda, Debarati, et al. "Evaluation of the Effect of Silver and Silver Nanoparticles On the Function of Selenoproteins Using an In-vitro Model of the Fish Intestine: the Cell Line RTgutGC." Ecotoxicology and Environmental Safety, vol. 211, 2021, p. 111930.
Chanda D, Dudefoi W, Anadu J, et al. Evaluation of the effect of silver and silver nanoparticles on the function of selenoproteins using an in-vitro model of the fish intestine: The cell line RTgutGC. Ecotoxicol Environ Saf. 2021;211:111930.
Chanda, D., Dudefoi, W., Anadu, J., & Minghetti, M. (2021). Evaluation of the effect of silver and silver nanoparticles on the function of selenoproteins using an in-vitro model of the fish intestine: The cell line RTgutGC. Ecotoxicology and Environmental Safety, 211, 111930. https://doi.org/10.1016/j.ecoenv.2021.111930
Chanda D, et al. Evaluation of the Effect of Silver and Silver Nanoparticles On the Function of Selenoproteins Using an In-vitro Model of the Fish Intestine: the Cell Line RTgutGC. Ecotoxicol Environ Saf. 2021 Mar 15;211:111930. PubMed PMID: 33472113.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Evaluation of the effect of silver and silver nanoparticles on the function of selenoproteins using an in-vitro model of the fish intestine: The cell line RTgutGC. AU - Chanda,Debarati, AU - Dudefoi,William, AU - Anadu,Joshua, AU - Minghetti,Matteo, Y1 - 2021/01/17/ PY - 2020/09/23/received PY - 2020/12/21/revised PY - 2021/01/10/accepted PY - 2021/1/21/pubmed PY - 2021/2/13/medline PY - 2021/1/20/entrez KW - AgNO3 KW - AgNP KW - Glutathione peroxidase KW - Oxidative stress KW - RTgutGC KW - Selenoproteins KW - Thioredoxin reductase SP - 111930 EP - 111930 JF - Ecotoxicology and environmental safety JO - Ecotoxicol Environ Saf VL - 211 N2 - Emerging research in mammalian cells suggests that ionic (AgNO3) and nano silver (AgNP) can disrupt the metabolism of selenium which plays a vital role in oxidative stress control. However, the effect of silver (Ag) on selenoprotein function in fish is poorly understood. Here we evaluate the effects of AgNO3 and citrate coated AgNP (cit-AgNP) on selenoprotein function and oxidative stress using a fish cell line derived from the rainbow trout (Oncorhynchus mykiss) intestine (RTgutGC). Cell viability was evaluated using a cytotoxicity assay which measures simultaneously metabolic activity, membrane integrity and lysosome integrity. Cells exposed to equimolar amounts of AgNO3 and cit-AgNP accumulated the same amount of silver intracellularly, however AgNO3 was more toxic than cit-AgNP. Selenoenzymes glutathione peroxidase (GPx) and thioredoxin reductase (TrxR) mRNA levels and enzyme activity were measured. While mRNA levels remained unaffected by AgNO3 or cit-AgNP, the enzyme activity of GPx was inhibited by AgNO3 (1 µM) and cit-AgNP (5 µM) and TrxR activity was inhibited by AgNO3 (0.4 µM) and cit-AgNP (1, 5 µM). Moreover, cells exposed to 1 µM of AgNO3 and cit-AgNP showed an increase in metallothionein b (MTb) mRNA levels at 24 h of exposure, confirming the uptake of silver, but returned to control levels at 72 h suggesting silver scavenging by MTb. Oxidative stress was not observed at any of the doses of AgNO3 or cit-AgNP tested. Overall, this study shows that AgNO3 or cit-AgNP can inhibit the activity of selenoenzymes but do not induce oxidative stress in RTgutGC cells. SN - 1090-2414 UR - https://www.unboundmedicine.com/medline/citation/33472113/Evaluation_of_the_effect_of_silver_and_silver_nanoparticles_on_the_function_of_selenoproteins_using_an_in_vitro_model_of_the_fish_intestine:_The_cell_line_RTgutGC_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0147-6513(21)00041-5 DB - PRIME DP - Unbound Medicine ER -