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Identification of cable bacteria and its biogeochemical impact on sulfur in freshwater sediments from the Wenyu River.
Sci Total Environ. 2021 May 15; 769:144541.ST

Abstract

Cable bacteria are filamentous sulfur-oxidizing microorganisms that couple the reduction of oxygen or nitrate in surface sediments with the oxidation of free sulfide in deeper sediments by transferring electrons across centimeter scale distances. The distribution and activities of cable bacteria in freshwater sediments are still poorly understood, especially the impact of cable bacteria on sulfur cycling. The goal of this study was to investigate electrogenic sulfide oxidation associated with cable bacteria in laboratory microcosm incubations of freshwater sediments using microsensor technology, 16S full-length rRNA sequencing, and fluorescence in situ hybridization (FISH) microscopy. Their activity was characterized by a pH maximum of 8.56 in the oxic zone and the formation of a 13.7 ± 0.6 mm wide suboxic zone after 25 days of incubation. Full-length 16S rRNA gene sequences related to cable bacteria were recovered from the sediments and exhibited 93.3%-99.4% nucleotide (nt) similarities with those from other reported freshwater cable bacteria, indicating that new species of cable bacteria were present in the sediments. FISH analysis indicated that cable bacteria density increased with time, reaching a maximum of 95.48 m cm-2 on day 50. The cells grew downwards to 40 mm but were mainly concentrated on the top 0-20 mm of sediment. The cable bacteria continuously consumed H2S in deeper layers and oxidized sulfide into sulfate in the 0-20 mm surface layers, thereby affecting the sulfur cycling within sediments. These findings provide new evidence for the existence of higher diversity of cable bacteria in freshwater sediments than previously known.

Authors+Show Affiliations

State Key Laboratory of Environmental Criteria and Risk Assessment, Chinese Research Academy of Environmental Science, Beijing 100012, China; College of Water Sciences, Beijing Normal University, Beijing 100012, China.State Key Laboratory of Environmental Criteria and Risk Assessment, Chinese Research Academy of Environmental Science, Beijing 100012, China. Electronic address: huosl@craes.org.cn.State Key Laboratory of Environmental Criteria and Risk Assessment, Chinese Research Academy of Environmental Science, Beijing 100012, China; College of Water Sciences, Beijing Normal University, Beijing 100012, China.State Key Laboratory of Environmental Criteria and Risk Assessment, Chinese Research Academy of Environmental Science, Beijing 100012, China.State Key Laboratory of Environmental Criteria and Risk Assessment, Chinese Research Academy of Environmental Science, Beijing 100012, China.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

33482557

Citation

Xu, Xiaoling, et al. "Identification of Cable Bacteria and Its Biogeochemical Impact On Sulfur in Freshwater Sediments From the Wenyu River." The Science of the Total Environment, vol. 769, 2021, p. 144541.
Xu X, Huo S, Zhang H, et al. Identification of cable bacteria and its biogeochemical impact on sulfur in freshwater sediments from the Wenyu River. Sci Total Environ. 2021;769:144541.
Xu, X., Huo, S., Zhang, H., Li, X., & Wu, F. (2021). Identification of cable bacteria and its biogeochemical impact on sulfur in freshwater sediments from the Wenyu River. The Science of the Total Environment, 769, 144541. https://doi.org/10.1016/j.scitotenv.2020.144541
Xu X, et al. Identification of Cable Bacteria and Its Biogeochemical Impact On Sulfur in Freshwater Sediments From the Wenyu River. Sci Total Environ. 2021 May 15;769:144541. PubMed PMID: 33482557.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Identification of cable bacteria and its biogeochemical impact on sulfur in freshwater sediments from the Wenyu River. AU - Xu,Xiaoling, AU - Huo,Shouliang, AU - Zhang,Hanxiao, AU - Li,Xiaochuang, AU - Wu,Fengchang, Y1 - 2021/01/14/ PY - 2020/08/28/received PY - 2020/11/25/revised PY - 2020/12/14/accepted PY - 2021/1/23/pubmed PY - 2021/3/19/medline PY - 2021/1/22/entrez KW - Biogeochemical cycling KW - Cable bacteria KW - Electrogenic activity KW - Sulfide oxidation KW - Sulfur SP - 144541 EP - 144541 JF - The Science of the total environment JO - Sci Total Environ VL - 769 N2 - Cable bacteria are filamentous sulfur-oxidizing microorganisms that couple the reduction of oxygen or nitrate in surface sediments with the oxidation of free sulfide in deeper sediments by transferring electrons across centimeter scale distances. The distribution and activities of cable bacteria in freshwater sediments are still poorly understood, especially the impact of cable bacteria on sulfur cycling. The goal of this study was to investigate electrogenic sulfide oxidation associated with cable bacteria in laboratory microcosm incubations of freshwater sediments using microsensor technology, 16S full-length rRNA sequencing, and fluorescence in situ hybridization (FISH) microscopy. Their activity was characterized by a pH maximum of 8.56 in the oxic zone and the formation of a 13.7 ± 0.6 mm wide suboxic zone after 25 days of incubation. Full-length 16S rRNA gene sequences related to cable bacteria were recovered from the sediments and exhibited 93.3%-99.4% nucleotide (nt) similarities with those from other reported freshwater cable bacteria, indicating that new species of cable bacteria were present in the sediments. FISH analysis indicated that cable bacteria density increased with time, reaching a maximum of 95.48 m cm-2 on day 50. The cells grew downwards to 40 mm but were mainly concentrated on the top 0-20 mm of sediment. The cable bacteria continuously consumed H2S in deeper layers and oxidized sulfide into sulfate in the 0-20 mm surface layers, thereby affecting the sulfur cycling within sediments. These findings provide new evidence for the existence of higher diversity of cable bacteria in freshwater sediments than previously known. SN - 1879-1026 UR - https://www.unboundmedicine.com/medline/citation/33482557/Identification_of_cable_bacteria_and_its_biogeochemical_impact_on_sulfur_in_freshwater_sediments_from_the_Wenyu_River_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0048-9697(20)38072-4 DB - PRIME DP - Unbound Medicine ER -