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Eco-Friendly and Facile Synthesis of Antioxidant, Antibacterial and Anticancer Dihydromyricetin-Mediated Silver Nanoparticles.
Int J Nanomedicine. 2021; 16:481-492.IJ

Abstract

Background

Dihydromyricetin (DMY), a natural flavonoid, has reportedly antibacterial, antioxidant, anticancer and other properties. In the present study, DMY was used as a reducing agent and stabilizer to synthesize silver nanoparticles (AgNPs), and the optimal conditions for its synthesis were studied. The DMY-AgNPs were investigated for their DPPH scavenging properties and their potential against human pathogenic and food-borne bacteria viz. Escherichia coli (E. coli), and Salmonella. In addition, DMY-AgNPs also showed excellent inhibitory effects on cancer Hela, HepG2 and MDA-MB-231 cell lines.

Methods

The dihydromyricetin-mediated AgNPs (DMY-AgNPs) were characterized by ultraviolet-visible spectrophotometer (UV-Vis spectra), scanning electron microscopy (SEM), transmission electron microscopy (TEM), dynamic light scattering (DLS), Fourier-transform infrared spectroscopy (FTIR), X-ray photoelectron spectroscopy (XPS), and X-ray powder diffraction (XRD). Antioxidant activity of DMY-AgNPs was determined by 1.1-diphenyl-2-picrylhydrazyl (DPPH) scavenging. The antibacterial activity was determined by 96-well plate (AGAR) gradient dilution, while anticancer potential was determined by MTT assay.

Results

The results showed that the dispersion of AgNPs had the maximum UV-visible absorption at about 410 nm. The synthesized nanoparticles were almost spherical. FTIR was used to identify functional groups that may lead to the transformation of metal ions into nanoparticles. The results showed that the prepared AgNPs were coated with biological molecules in the extraction solution. The biosynthesized DMY-AgNPs exhibited good antioxidant properties, at various concentrations (0.01-0.1mg/mL), the free radical scavenging rate was about 56-92%. Furthermore, DMY-AgNPs possessed good antibacterial properties against Escherichia coli (E. coli), and Salmonella at room temperature. The minimum inhibitory concentrations (MIC) were 10-6 g/L, and 10-4 g/L, respectively. The bioactivity of DMY-mediated AgNPs was studied using MTT assay against Hela, HepG2 and MDA-MB-231 cancer cell lines, and all showed good inhibitory effects.

Conclusion

The present study provides a green approach for the synthesis of DMY-AgNPs which exhibited stronger antioxidant, antibacterial and anticancer properties compared to the dihydromyricetin. DMY-AgNPs can serve as an economical, efficient, and effective antimicrobial material for its applications in food and pharmaceutical fields.

Authors+Show Affiliations

Shandong Analysis and Test Center, Qilu University of Technology (Shandong Academy of Sciences), Jinan 250014, People's Republic of China. College of Life Science, Shandong Normal University, Jinan 250014, People's Republic of China.Shandong Analysis and Test Center, Qilu University of Technology (Shandong Academy of Sciences), Jinan 250014, People's Republic of China. Department of Chemistry, Karakoram International University, Gilgit 15100, Pakistan.Institute of Agro-Food Science and Technology, Shandong Academy of Agricultural Sciences, Jinan 250100, People's Republic of China.Shandong Analysis and Test Center, Qilu University of Technology (Shandong Academy of Sciences), Jinan 250014, People's Republic of China.Shandong Analysis and Test Center, Qilu University of Technology (Shandong Academy of Sciences), Jinan 250014, People's Republic of China. College of Life Science, Shandong Normal University, Jinan 250014, People's Republic of China.Jinan Municipal Center for Disease Control and Prevention, Jinan 250001, People's Republic of China.Shandong Analysis and Test Center, Qilu University of Technology (Shandong Academy of Sciences), Jinan 250014, People's Republic of China.Shandong Analysis and Test Center, Qilu University of Technology (Shandong Academy of Sciences), Jinan 250014, People's Republic of China.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

33500618

Citation

Li, Zhao, et al. "Eco-Friendly and Facile Synthesis of Antioxidant, Antibacterial and Anticancer Dihydromyricetin-Mediated Silver Nanoparticles." International Journal of Nanomedicine, vol. 16, 2021, pp. 481-492.
Li Z, Ali I, Qiu J, et al. Eco-Friendly and Facile Synthesis of Antioxidant, Antibacterial and Anticancer Dihydromyricetin-Mediated Silver Nanoparticles. Int J Nanomedicine. 2021;16:481-492.
Li, Z., Ali, I., Qiu, J., Zhao, H., Ma, W., Bai, A., Wang, D., & Li, J. (2021). Eco-Friendly and Facile Synthesis of Antioxidant, Antibacterial and Anticancer Dihydromyricetin-Mediated Silver Nanoparticles. International Journal of Nanomedicine, 16, 481-492. https://doi.org/10.2147/IJN.S283677
Li Z, et al. Eco-Friendly and Facile Synthesis of Antioxidant, Antibacterial and Anticancer Dihydromyricetin-Mediated Silver Nanoparticles. Int J Nanomedicine. 2021;16:481-492. PubMed PMID: 33500618.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Eco-Friendly and Facile Synthesis of Antioxidant, Antibacterial and Anticancer Dihydromyricetin-Mediated Silver Nanoparticles. AU - Li,Zhao, AU - Ali,Iftikhar, AU - Qiu,Jiying, AU - Zhao,Huanzhu, AU - Ma,Wenya, AU - Bai,Aiying, AU - Wang,Daijie, AU - Li,Jingchao, Y1 - 2021/01/19/ PY - 2020/09/23/received PY - 2020/12/22/accepted PY - 2021/1/27/entrez PY - 2021/1/28/pubmed PY - 2021/2/12/medline KW - DMY-AgNO3-NPs KW - DPPH KW - Escherichia coli KW - Hela KW - HepG2 KW - MDA-MB-231 KW - Salmonella KW - green approach KW - natural flavonoid SP - 481 EP - 492 JF - International journal of nanomedicine JO - Int J Nanomedicine VL - 16 N2 - Background: Dihydromyricetin (DMY), a natural flavonoid, has reportedly antibacterial, antioxidant, anticancer and other properties. In the present study, DMY was used as a reducing agent and stabilizer to synthesize silver nanoparticles (AgNPs), and the optimal conditions for its synthesis were studied. The DMY-AgNPs were investigated for their DPPH scavenging properties and their potential against human pathogenic and food-borne bacteria viz. Escherichia coli (E. coli), and Salmonella. In addition, DMY-AgNPs also showed excellent inhibitory effects on cancer Hela, HepG2 and MDA-MB-231 cell lines. Methods: The dihydromyricetin-mediated AgNPs (DMY-AgNPs) were characterized by ultraviolet-visible spectrophotometer (UV-Vis spectra), scanning electron microscopy (SEM), transmission electron microscopy (TEM), dynamic light scattering (DLS), Fourier-transform infrared spectroscopy (FTIR), X-ray photoelectron spectroscopy (XPS), and X-ray powder diffraction (XRD). Antioxidant activity of DMY-AgNPs was determined by 1.1-diphenyl-2-picrylhydrazyl (DPPH) scavenging. The antibacterial activity was determined by 96-well plate (AGAR) gradient dilution, while anticancer potential was determined by MTT assay. Results: The results showed that the dispersion of AgNPs had the maximum UV-visible absorption at about 410 nm. The synthesized nanoparticles were almost spherical. FTIR was used to identify functional groups that may lead to the transformation of metal ions into nanoparticles. The results showed that the prepared AgNPs were coated with biological molecules in the extraction solution. The biosynthesized DMY-AgNPs exhibited good antioxidant properties, at various concentrations (0.01-0.1mg/mL), the free radical scavenging rate was about 56-92%. Furthermore, DMY-AgNPs possessed good antibacterial properties against Escherichia coli (E. coli), and Salmonella at room temperature. The minimum inhibitory concentrations (MIC) were 10-6 g/L, and 10-4 g/L, respectively. The bioactivity of DMY-mediated AgNPs was studied using MTT assay against Hela, HepG2 and MDA-MB-231 cancer cell lines, and all showed good inhibitory effects. Conclusion: The present study provides a green approach for the synthesis of DMY-AgNPs which exhibited stronger antioxidant, antibacterial and anticancer properties compared to the dihydromyricetin. DMY-AgNPs can serve as an economical, efficient, and effective antimicrobial material for its applications in food and pharmaceutical fields. SN - 1178-2013 UR - https://www.unboundmedicine.com/medline/citation/33500618/Eco_Friendly_and_Facile_Synthesis_of_Antioxidant_Antibacterial_and_Anticancer_Dihydromyricetin_Mediated_Silver_Nanoparticles_ L2 - https://dx.doi.org/10.2147/IJN.S283677 DB - PRIME DP - Unbound Medicine ER -