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A new method to detect lymphocytic choriomeningitis virus-specific antibody in human sera.
J Gen Virol. 1977 Oct; 37(1):85-92.JG

Abstract

A plaque reduction method for measuring lymphocytic choriomeningitis virus-sensitizing antibody in human serum is described. One volume of virus and one volume of serially diluted human serum were mixed and incubated for 2 h at 37 degrees C. One volume of suitably diluted anti-human immune globulin antiserum was added and incubation continued for 0-5 h. Residual infectivity was then determined by means of a plaque assay employing L cell monolayer cultures and a methyl cellulose containing overlay medium. Of 75 sera from as many persons, 20 were positive with titres ranging from 640 to 10240. All positive sera were from verified cases or from persons who had had occupational contact with the virus. Close correlation of results was found between this method and a neutralization test employing mice, the one exception indicating that the in vitro assay for sensitizing antibody is more sensitive than the mouse assay for neutralizing antibody.

Authors

No affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article

Language

eng

PubMed ID

335024

Citation

Lehmann-Grube, F, and J Ambrassat. "A New Method to Detect Lymphocytic Choriomeningitis Virus-specific Antibody in Human Sera." The Journal of General Virology, vol. 37, no. 1, 1977, pp. 85-92.
Lehmann-Grube F, Ambrassat J. A new method to detect lymphocytic choriomeningitis virus-specific antibody in human sera. J Gen Virol. 1977;37(1):85-92.
Lehmann-Grube, F., & Ambrassat, J. (1977). A new method to detect lymphocytic choriomeningitis virus-specific antibody in human sera. The Journal of General Virology, 37(1), 85-92.
Lehmann-Grube F, Ambrassat J. A New Method to Detect Lymphocytic Choriomeningitis Virus-specific Antibody in Human Sera. J Gen Virol. 1977;37(1):85-92. PubMed PMID: 335024.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - A new method to detect lymphocytic choriomeningitis virus-specific antibody in human sera. AU - Lehmann-Grube,F, AU - Ambrassat,J, PY - 1977/10/1/pubmed PY - 1977/10/1/medline PY - 1977/10/1/entrez SP - 85 EP - 92 JF - The Journal of general virology JO - J Gen Virol VL - 37 IS - 1 N2 - A plaque reduction method for measuring lymphocytic choriomeningitis virus-sensitizing antibody in human serum is described. One volume of virus and one volume of serially diluted human serum were mixed and incubated for 2 h at 37 degrees C. One volume of suitably diluted anti-human immune globulin antiserum was added and incubation continued for 0-5 h. Residual infectivity was then determined by means of a plaque assay employing L cell monolayer cultures and a methyl cellulose containing overlay medium. Of 75 sera from as many persons, 20 were positive with titres ranging from 640 to 10240. All positive sera were from verified cases or from persons who had had occupational contact with the virus. Close correlation of results was found between this method and a neutralization test employing mice, the one exception indicating that the in vitro assay for sensitizing antibody is more sensitive than the mouse assay for neutralizing antibody. SN - 0022-1317 UR - https://www.unboundmedicine.com/medline/citation/335024/A_new_method_to_detect_lymphocytic_choriomeningitis_virus_specific_antibody_in_human_sera_ L2 - http://jgv.microbiologyresearch.org/pubmed/content/journal/jgv/10.1099/0022-1317-37-1-85 DB - PRIME DP - Unbound Medicine ER -