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Differential MHC class II expression on human peripheral blood monocytes and dendritic cells.
Immunology. 1988 Feb; 63(2):303-11.I

Abstract

Both monocytes (MO) and dendritic cells (DC) in human peripheral blood are of a plastic-adherent nature. The expression of the MHC class II sublocus products HLA-DP, -DQ and -DR on human peripheral blood transiently adherent cells (TA) was examined by an immunocytochemical staining technique. While most TA showed strong expression of molecules of the HLA-DR subtype, only a small proportion of cells (2-6%) showed strong HLA-DP or -DQ positivity. This strong expression of the HLA-DP and HLA-DQ sublocus products by a subset of TA was seen only after short-term culture; freshly isolated cells expressed comparatively low levels of these molecules. Enrichment for Fc receptor-negative or low-density cells from TA produced populations with strong HLA-DQ and -DP expression. Such co-enrichment of the strongly HLA-DQ+ and strongly HLA-DP+ cells suggests that the same cells express high levels of both types of MHC class II molecule. Immunocytochemical analysis of TA indicated that the strongly HLA-DQ+ cells, at least, were only weakly or non-reactive with the MO-specific monoclonal antibodies OKM1, UCHM1, MO2 and EB11. In addition, strongly HLA-DQ- or -DP-positive cells were poorly phagocytic in comparison with the majority of adherent cells. The apparent FcR-negative, low-density and weakly phagocytic nature of the strongly HLA-DQ/DP+ cells, combined with their lack of reactivity with several MO-specific antibodies, suggests that they may represent the DC component of TA. Such strong HLA-DQ/DP expression by DC may aid their positive identification in human peripheral blood and may be of relevance to DC function in antigen presentation.

Authors+Show Affiliations

Paterson Institute for Cancer Research, Christie Hospital and Holt Radium Institute, Manchester, U.K.No affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

3350576

Citation

Brooks, C F., and M Moore. "Differential MHC Class II Expression On Human Peripheral Blood Monocytes and Dendritic Cells." Immunology, vol. 63, no. 2, 1988, pp. 303-11.
Brooks CF, Moore M. Differential MHC class II expression on human peripheral blood monocytes and dendritic cells. Immunology. 1988;63(2):303-11.
Brooks, C. F., & Moore, M. (1988). Differential MHC class II expression on human peripheral blood monocytes and dendritic cells. Immunology, 63(2), 303-11.
Brooks CF, Moore M. Differential MHC Class II Expression On Human Peripheral Blood Monocytes and Dendritic Cells. Immunology. 1988;63(2):303-11. PubMed PMID: 3350576.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Differential MHC class II expression on human peripheral blood monocytes and dendritic cells. AU - Brooks,C F, AU - Moore,M, PY - 1988/2/1/pubmed PY - 1988/2/1/medline PY - 1988/2/1/entrez SP - 303 EP - 11 JF - Immunology JO - Immunology VL - 63 IS - 2 N2 - Both monocytes (MO) and dendritic cells (DC) in human peripheral blood are of a plastic-adherent nature. The expression of the MHC class II sublocus products HLA-DP, -DQ and -DR on human peripheral blood transiently adherent cells (TA) was examined by an immunocytochemical staining technique. While most TA showed strong expression of molecules of the HLA-DR subtype, only a small proportion of cells (2-6%) showed strong HLA-DP or -DQ positivity. This strong expression of the HLA-DP and HLA-DQ sublocus products by a subset of TA was seen only after short-term culture; freshly isolated cells expressed comparatively low levels of these molecules. Enrichment for Fc receptor-negative or low-density cells from TA produced populations with strong HLA-DQ and -DP expression. Such co-enrichment of the strongly HLA-DQ+ and strongly HLA-DP+ cells suggests that the same cells express high levels of both types of MHC class II molecule. Immunocytochemical analysis of TA indicated that the strongly HLA-DQ+ cells, at least, were only weakly or non-reactive with the MO-specific monoclonal antibodies OKM1, UCHM1, MO2 and EB11. In addition, strongly HLA-DQ- or -DP-positive cells were poorly phagocytic in comparison with the majority of adherent cells. The apparent FcR-negative, low-density and weakly phagocytic nature of the strongly HLA-DQ/DP+ cells, combined with their lack of reactivity with several MO-specific antibodies, suggests that they may represent the DC component of TA. Such strong HLA-DQ/DP expression by DC may aid their positive identification in human peripheral blood and may be of relevance to DC function in antigen presentation. SN - 0019-2805 UR - https://www.unboundmedicine.com/medline/citation/3350576/Differential_MHC_class_II_expression_on_human_peripheral_blood_monocytes_and_dendritic_cells_ L2 - https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/3350576/ DB - PRIME DP - Unbound Medicine ER -