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The stability and immunogenicity of a dispersed-grown freeze-dried Pasteur BCG vaccine.
J Biol Stand 1988; 16(1):15-26JB

Abstract

The level of antituberculous immunity seems to be related to the number of memory T cells induced. This may vary as a function of the multiplication and persistence of BCG in host tissues. The most important requirements for a BCG vaccine are, therefore, the immunogenicity of the strain, the high proportion of live to dead bacilli, and adequate dispersion and low levels of soluble antigens. The surface-grown Pasteur BCG vaccine contains a very high proportion of bacilli killed by ball-milling and freeze-drying. It also contains clumps and soluble antigens, all factors influencing cell-mediated immune processes and viability control. Therefore, several batches of vaccine were prepared on an industrial scale using one of the most immunogenic strains (French 1173 P2) and grown as dispersed bacilli by a modified cell type culture method. This method provided fully viable, well-dispersed vaccines which have a viability and heat stability superior to that of the classical surface-grown BCG. The immunogenicity was checked by multiplication and persistence in mouse organs and the skin reactivity and tuberculin hypersensitivity in guinea-pigs showed results comparable to those obtained with classical vaccine. Small-scale tests in children showed superior immunogenicity of the dispersed as opposed to the classical vaccine and there was no suppurative adenitis.

Authors+Show Affiliations

BCG Laboratory, Pasteur Institute, Paris, France.No affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article

Language

eng

PubMed ID

3350821

Citation

Gheorghiu, M, et al. "The Stability and Immunogenicity of a Dispersed-grown Freeze-dried Pasteur BCG Vaccine." Journal of Biological Standardization, vol. 16, no. 1, 1988, pp. 15-26.
Gheorghiu M, Lagrange PH, Fillastre C. The stability and immunogenicity of a dispersed-grown freeze-dried Pasteur BCG vaccine. J Biol Stand. 1988;16(1):15-26.
Gheorghiu, M., Lagrange, P. H., & Fillastre, C. (1988). The stability and immunogenicity of a dispersed-grown freeze-dried Pasteur BCG vaccine. Journal of Biological Standardization, 16(1), pp. 15-26.
Gheorghiu M, Lagrange PH, Fillastre C. The Stability and Immunogenicity of a Dispersed-grown Freeze-dried Pasteur BCG Vaccine. J Biol Stand. 1988;16(1):15-26. PubMed PMID: 3350821.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The stability and immunogenicity of a dispersed-grown freeze-dried Pasteur BCG vaccine. AU - Gheorghiu,M, AU - Lagrange,P H, AU - Fillastre,C, PY - 1988/1/1/pubmed PY - 1988/1/1/medline PY - 1988/1/1/entrez SP - 15 EP - 26 JF - Journal of biological standardization JO - J Biol Stand VL - 16 IS - 1 N2 - The level of antituberculous immunity seems to be related to the number of memory T cells induced. This may vary as a function of the multiplication and persistence of BCG in host tissues. The most important requirements for a BCG vaccine are, therefore, the immunogenicity of the strain, the high proportion of live to dead bacilli, and adequate dispersion and low levels of soluble antigens. The surface-grown Pasteur BCG vaccine contains a very high proportion of bacilli killed by ball-milling and freeze-drying. It also contains clumps and soluble antigens, all factors influencing cell-mediated immune processes and viability control. Therefore, several batches of vaccine were prepared on an industrial scale using one of the most immunogenic strains (French 1173 P2) and grown as dispersed bacilli by a modified cell type culture method. This method provided fully viable, well-dispersed vaccines which have a viability and heat stability superior to that of the classical surface-grown BCG. The immunogenicity was checked by multiplication and persistence in mouse organs and the skin reactivity and tuberculin hypersensitivity in guinea-pigs showed results comparable to those obtained with classical vaccine. Small-scale tests in children showed superior immunogenicity of the dispersed as opposed to the classical vaccine and there was no suppurative adenitis. SN - 0092-1157 UR - https://www.unboundmedicine.com/medline/citation/3350821/The_stability_and_immunogenicity_of_a_dispersed_grown_freeze_dried_Pasteur_BCG_vaccine_ L2 - https://medlineplus.gov/tuberculosis.html DB - PRIME DP - Unbound Medicine ER -