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Karyotyping Dasypyrum breviaristatum chromosomes with multiple oligonucleotide probes reveals the genomic divergence in Dasypyrum.
Genome. 2021 Aug; 64(8):789-800.G

Abstract

The perennial species Dasypyrum breviaristatum (genome Vb) contains many potentially valuable genes for the improvement of common wheat. Construction of a detailed karyotype of D. breviaristatum chromosomes will be useful for the detection of Dasypyrum chromatin in wheat background. We established the standard karyotype of 1Vb-7Vb chromosomes through nondenaturing fluorescence in situ hybridization (ND-FISH) technique using 28 oligonucleotide probes from the wheat - D. breviaristatum partial amphiploid TDH-2 (AABBVbVb) and newly identified wheat - D. breviaristatum disomic translocation and addition lines D2138 (6VbS.2VbL), D2547 (4Vb), and D2532 (3VbS.6VbL) by comparative molecular marker analysis. The ND-FISH with multiple oligo probes was conducted on the durum wheat - D. villosum amphiploid TDV-1 and large karyotype differences between D. breviaristatum and D. villosum was revealed. These ND-FISH probes will be valuable for screening the wheat - Dasypyrum derivative lines for chromosome identification, and the newly developed wheat - D. breviaristatum addition lines may broaden the gene pool of wheat breeding. The differences between D. villosum and D. breviaristatum chromosomes revealed by ND-FISH will help us understand evolutionary divergence of repetitive sequences within the genus Dasypyrum.

Authors+Show Affiliations

Center for Informational Biology, School of Life Science and Technology, University of Electronic and Technology of China, Chengdu 611731, China. Center for Informational Biology, School of Life Science and Technology, University of Electronic and Technology of China, Chengdu 611731, China.Center for Informational Biology, School of Life Science and Technology, University of Electronic and Technology of China, Chengdu 611731, China. Center for Informational Biology, School of Life Science and Technology, University of Electronic and Technology of China, Chengdu 611731, China.Center for Informational Biology, School of Life Science and Technology, University of Electronic and Technology of China, Chengdu 611731, China. Center for Informational Biology, School of Life Science and Technology, University of Electronic and Technology of China, Chengdu 611731, China.Center for Informational Biology, School of Life Science and Technology, University of Electronic and Technology of China, Chengdu 611731, China. Center for Informational Biology, School of Life Science and Technology, University of Electronic and Technology of China, Chengdu 611731, China.Center for Informational Biology, School of Life Science and Technology, University of Electronic and Technology of China, Chengdu 611731, China. Center for Informational Biology, School of Life Science and Technology, University of Electronic and Technology of China, Chengdu 611731, China.Center for Informational Biology, School of Life Science and Technology, University of Electronic and Technology of China, Chengdu 611731, China. Center for Informational Biology, School of Life Science and Technology, University of Electronic and Technology of China, Chengdu 611731, China.Center for Informational Biology, School of Life Science and Technology, University of Electronic and Technology of China, Chengdu 611731, China. Center for Informational Biology, School of Life Science and Technology, University of Electronic and Technology of China, Chengdu 611731, China.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

33513072

Citation

Yu, Zhihui, et al. "Karyotyping Dasypyrum Breviaristatum Chromosomes With Multiple Oligonucleotide Probes Reveals the Genomic Divergence in Dasypyrum." Genome, vol. 64, no. 8, 2021, pp. 789-800.
Yu Z, Wang H, Jiang W, et al. Karyotyping Dasypyrum breviaristatum chromosomes with multiple oligonucleotide probes reveals the genomic divergence in Dasypyrum. Genome. 2021;64(8):789-800.
Yu, Z., Wang, H., Jiang, W., Jiang, C., Yuan, W., Li, G., & Yang, Z. (2021). Karyotyping Dasypyrum breviaristatum chromosomes with multiple oligonucleotide probes reveals the genomic divergence in Dasypyrum. Genome, 64(8), 789-800. https://doi.org/10.1139/gen-2020-0147
Yu Z, et al. Karyotyping Dasypyrum Breviaristatum Chromosomes With Multiple Oligonucleotide Probes Reveals the Genomic Divergence in Dasypyrum. Genome. 2021;64(8):789-800. PubMed PMID: 33513072.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Karyotyping Dasypyrum breviaristatum chromosomes with multiple oligonucleotide probes reveals the genomic divergence in Dasypyrum. AU - Yu,Zhihui, AU - Wang,Hongjin, AU - Jiang,Wenxi, AU - Jiang,Chengzhi, AU - Yuan,Weiguang, AU - Li,Guangrong, AU - Yang,Zujun, Y1 - 2021/01/29/ PY - 2021/1/30/pubmed PY - 2021/1/30/medline PY - 2021/1/29/entrez KW - Dasypyrum breviaristatum KW - Dasypyrum villosum KW - ND-FISH KW - chromosome identification KW - identification des chromosomes KW - oligo probes KW - sondes oligonucléotidiques SP - 789 EP - 800 JF - Genome JO - Genome VL - 64 IS - 8 N2 - The perennial species Dasypyrum breviaristatum (genome Vb) contains many potentially valuable genes for the improvement of common wheat. Construction of a detailed karyotype of D. breviaristatum chromosomes will be useful for the detection of Dasypyrum chromatin in wheat background. We established the standard karyotype of 1Vb-7Vb chromosomes through nondenaturing fluorescence in situ hybridization (ND-FISH) technique using 28 oligonucleotide probes from the wheat - D. breviaristatum partial amphiploid TDH-2 (AABBVbVb) and newly identified wheat - D. breviaristatum disomic translocation and addition lines D2138 (6VbS.2VbL), D2547 (4Vb), and D2532 (3VbS.6VbL) by comparative molecular marker analysis. The ND-FISH with multiple oligo probes was conducted on the durum wheat - D. villosum amphiploid TDV-1 and large karyotype differences between D. breviaristatum and D. villosum was revealed. These ND-FISH probes will be valuable for screening the wheat - Dasypyrum derivative lines for chromosome identification, and the newly developed wheat - D. breviaristatum addition lines may broaden the gene pool of wheat breeding. The differences between D. villosum and D. breviaristatum chromosomes revealed by ND-FISH will help us understand evolutionary divergence of repetitive sequences within the genus Dasypyrum. SN - 1480-3321 UR - https://www.unboundmedicine.com/medline/citation/33513072/Karyotyping_Dasypyrum_breviaristatum_chromosomes_with_multiple_oligonucleotide_probes_reveals_the_genomic_divergence_in_Dasypyrum_ L2 - https://cdnsciencepub.com/doi/10.1139/gen-2020-0147?url_ver=Z39.88-2003&rfr_id=ori:rid:crossref.org&rfr_dat=cr_pub=pubmed DB - PRIME DP - Unbound Medicine ER -