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Polysaccharide from Angelica sinensis attenuates SNP-induced apoptosis in osteoarthritis chondrocytes by inducing autophagy via the ERK1/2 pathway.
Arthritis Res Ther. 2021 01 30; 23(1):47.AR

Abstract

OBJECTIVE

Chondrocyte apoptosis plays a vital role in osteoarthritis (OA) progression. Angelica sinensis polysaccharide (ASP), a traditional Chinese medicine, possesses anti-inflammatory and anti-apoptotic properties in chondrocytes. This study aimed to determine the protective role of ASP on sodium nitroprusside (SNP)-induced chondrocyte apoptosis, and explore the underlying mechanism.

METHOD

Human primary chondrocytes isolated from the articular cartilage of OA patients were treated with SNP alone or in combination with different doses of ASP. Cell viability and apoptosis were assessed, and apoptosis-related proteins including Bcl-2 and Bax were detected. Autophagy levels were evaluated by light chain 3 (LC3) II immunofluorescence staining, mRFP-GFP-LC3 fluorescence localization, and western blot (LC3II, p62, Beclin-1, Atg5). Meanwhile, activation of the ERK 1/2 pathway was determined by western blot. The autophagy inhibitors, 3-methyladenine (3-MA), chloroquine (CQ), and a specific inhibitor of ERK1/2, SCH772984, were used to confirm the autophagic effect of ASP.

RESULTS

The results showed that SNP-induced chondrocyte apoptosis was significantly rescued by ASP, whereas ASP alone promoted chondrocyte proliferation. The anti-apoptotic effect of ASP was related to the enhanced autophagy and depended on the activation of the ERK1/2 pathway.

CONCLUSION

ASP markedly rescued SNP-induced apoptosis by activating ERK1/2-dependent autophagy in chondrocytes, and it made ASP as a potential therapeutic supplementation for OA treatment.

Authors+Show Affiliations

Trauma Center, The Affiliated Changzhou No.2 People's Hospital of Nanjing Medical University, 29 Xinglong Alley, Changzhou, 213003, China.Medical Research Center, The Affiliated Changzhou No.2 People's Hospital of Nanjing Medical University, 29 Xinglong Alley, Changzhou, 213003, China.Department of Orthopedics, The Affiliated Changzhou No.2 People's Hospital of Nanjing Medical University, 29 Xinglong Alley, Changzhou, 213003, China.Medical Research Center, The Affiliated Changzhou No.2 People's Hospital of Nanjing Medical University, 29 Xinglong Alley, Changzhou, 213003, China.Department of Orthopedics, The Affiliated Changzhou No.2 People's Hospital of Nanjing Medical University, 29 Xinglong Alley, Changzhou, 213003, China.Department of Orthopedics, The Affiliated Changzhou No.2 People's Hospital of Nanjing Medical University, 29 Xinglong Alley, Changzhou, 213003, China.Department of Orthopedics, The Affiliated Changzhou No.2 People's Hospital of Nanjing Medical University, 29 Xinglong Alley, Changzhou, 213003, China.Department of Orthopedics, The Affiliated Changzhou No.2 People's Hospital of Nanjing Medical University, 29 Xinglong Alley, Changzhou, 213003, China.Department of Orthopedic Surgery and Biochemistry & Molecular Biology, Mayo Clinic, Rochester, MN, USA. vanwijnen.andre@mayo.edu.Department of Orthopedics, The Affiliated Changzhou No.2 People's Hospital of Nanjing Medical University, 29 Xinglong Alley, Changzhou, 213003, China. yujiwang@sohu.com. Department of Orthopedic Surgery and Biochemistry & Molecular Biology, Mayo Clinic, Rochester, MN, USA. yujiwang@sohu.com. Department of Orthopedics, The Third Affiliated Hospital of Gansu University of Chinese Medicine, 222 Silong Road, Baiyin, 730900, China. yujiwang@sohu.com.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

33514407

Citation

Xu, Chao, et al. "Polysaccharide From Angelica Sinensis Attenuates SNP-induced Apoptosis in Osteoarthritis Chondrocytes By Inducing Autophagy Via the ERK1/2 Pathway." Arthritis Research & Therapy, vol. 23, no. 1, 2021, p. 47.
Xu C, Ni S, Zhuang C, et al. Polysaccharide from Angelica sinensis attenuates SNP-induced apoptosis in osteoarthritis chondrocytes by inducing autophagy via the ERK1/2 pathway. Arthritis Res Ther. 2021;23(1):47.
Xu, C., Ni, S., Zhuang, C., Li, C., Zhao, G., Jiang, S., Wang, L., Zhu, R., van Wijnen, A. J., & Wang, Y. (2021). Polysaccharide from Angelica sinensis attenuates SNP-induced apoptosis in osteoarthritis chondrocytes by inducing autophagy via the ERK1/2 pathway. Arthritis Research & Therapy, 23(1), 47. https://doi.org/10.1186/s13075-020-02409-3
Xu C, et al. Polysaccharide From Angelica Sinensis Attenuates SNP-induced Apoptosis in Osteoarthritis Chondrocytes By Inducing Autophagy Via the ERK1/2 Pathway. Arthritis Res Ther. 2021 01 30;23(1):47. PubMed PMID: 33514407.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Polysaccharide from Angelica sinensis attenuates SNP-induced apoptosis in osteoarthritis chondrocytes by inducing autophagy via the ERK1/2 pathway. AU - Xu,Chao, AU - Ni,Su, AU - Zhuang,Chao, AU - Li,Chenkai, AU - Zhao,Gongyin, AU - Jiang,Shijie, AU - Wang,Liangliang, AU - Zhu,Ruixia, AU - van Wijnen,Andre J, AU - Wang,Yuji, Y1 - 2021/01/30/ PY - 2020/05/14/received PY - 2020/12/26/accepted PY - 2021/1/30/entrez PY - 2021/1/31/pubmed PY - 2021/6/22/medline KW - Angelica sinensis polysaccharide KW - Autophagy KW - Chondrocytes KW - Osteoarthritis SP - 47 EP - 47 JF - Arthritis research & therapy JO - Arthritis Res Ther VL - 23 IS - 1 N2 - OBJECTIVE: Chondrocyte apoptosis plays a vital role in osteoarthritis (OA) progression. Angelica sinensis polysaccharide (ASP), a traditional Chinese medicine, possesses anti-inflammatory and anti-apoptotic properties in chondrocytes. This study aimed to determine the protective role of ASP on sodium nitroprusside (SNP)-induced chondrocyte apoptosis, and explore the underlying mechanism. METHOD: Human primary chondrocytes isolated from the articular cartilage of OA patients were treated with SNP alone or in combination with different doses of ASP. Cell viability and apoptosis were assessed, and apoptosis-related proteins including Bcl-2 and Bax were detected. Autophagy levels were evaluated by light chain 3 (LC3) II immunofluorescence staining, mRFP-GFP-LC3 fluorescence localization, and western blot (LC3II, p62, Beclin-1, Atg5). Meanwhile, activation of the ERK 1/2 pathway was determined by western blot. The autophagy inhibitors, 3-methyladenine (3-MA), chloroquine (CQ), and a specific inhibitor of ERK1/2, SCH772984, were used to confirm the autophagic effect of ASP. RESULTS: The results showed that SNP-induced chondrocyte apoptosis was significantly rescued by ASP, whereas ASP alone promoted chondrocyte proliferation. The anti-apoptotic effect of ASP was related to the enhanced autophagy and depended on the activation of the ERK1/2 pathway. CONCLUSION: ASP markedly rescued SNP-induced apoptosis by activating ERK1/2-dependent autophagy in chondrocytes, and it made ASP as a potential therapeutic supplementation for OA treatment. SN - 1478-6362 UR - https://www.unboundmedicine.com/medline/citation/33514407/Polysaccharide_from_Angelica_sinensis_attenuates_SNP-induced_apoptosis_in_osteoarthritis_chondrocytes_by_inducing_autophagy_via_the_ERK1/2_pathway. L2 - https://arthritis-research.biomedcentral.com/articles/10.1186/s13075-020-02409-3 DB - PRIME DP - Unbound Medicine ER -