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Quantitation and Validation of 34 Fentanyl Analogs from Liver Tissue Using a QuEChERS Extraction and LC-MS-MS Analysis.
J Anal Toxicol. 2021 Jan 30 [Online ahead of print]JA

Abstract

Since 2013, drug overdose deaths involving synthetic opioids (including fentanyl and fentanyl analogs) have increased from 3,105 to 31,335 in 2018. Postmortem toxicological analysis in fentanyl-related overdose deaths is complicated by the high potency of the drug, often resulting in low analyte concentrations and associations with toxicity, multidrug use, novelty of emerging fentanyl analogs and postmortem redistribution. Objectives for this study include the development of a quick, easy, cheap, effective, rugged and safe (QuEChERS) extraction and subsequent liquid chromatography-mass spectrometry/mass spectrometry analysis, validation of the method following the American Academy of Forensic Sciences Standards Board (ASB) standard 036 requirements and application to authentic liver specimens for 34 analytes including fentanyl, metabolites and fentanyl analogs. The bias for all 34 fentanyl analogs did not exceed ±10% for any of the low, medium or high concentrations and the %CV did not exceed 20%. No interferences were identified. All 34 analytes were within the criteria for acceptable percent ionization suppression or enhancement with the low concentration ranging from -10.2% to 23.7% and the high concentration ranging from -7.1% to 11.0%. Liver specimens from 22 authentic postmortem cases were extracted and analyzed with all samples being positive for at least one target analyte from the 34 compounds. Of the 22 samples, 17 contained fentanyl and metabolites plus at least one fentanyl analog. The highest concentration for a fentanyl analog was 541.4 μg/kg of para-fluoroisobutyryl fentanyl (FIBF). The concentrations for fentanyl (n = 20) ranged between 3.6 and 164.9 μg/kg with a mean of 54.7 μg/kg. The fentanyl analog that was most encountered was methoxyacetyl fentanyl (n = 11) with a range of 0.2-4.6 μg/kg and a mean of 1.3 μg/kg. The QuEChERS extraction was fully validated using the ASB Standard 036 requirements for fentanyl, metabolites and fentanyl analogs in liver tissue.

Authors+Show Affiliations

Department of Forensic and Investigative Science, 1600 University Avenue, Oglebay Hall, Room 302, Morgantown, WV 26506, USA.Department of Forensic and Investigative Science, 1600 University Avenue, Oglebay Hall, Room 302, Morgantown, WV 26506, USA.Department of Forensic and Investigative Science, 1600 University Avenue, Oglebay Hall, Room 302, Morgantown, WV 26506, USA.Department of Pathology, Allegheny General Hospital, 320 E North Avenue, Pittsburgh, PA 15212, USA.Office of the Chief Medical Examiner, 619 Virginia Street West, Charleston, WV 25302, USA.Department of Chemistry and Biochemistry, Florida International University (FIU), 11200 SW 8th street, Miami, FL 33199, USA.Department of Forensic and Investigative Science, 1600 University Avenue, Oglebay Hall, Room 302, Morgantown, WV 26506, USA.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

33515247

Citation

Cox, Joseph, et al. "Quantitation and Validation of 34 Fentanyl Analogs From Liver Tissue Using a QuEChERS Extraction and LC-MS-MS Analysis." Journal of Analytical Toxicology, 2021.
Cox J, Mathison K, Ott C, et al. Quantitation and Validation of 34 Fentanyl Analogs from Liver Tissue Using a QuEChERS Extraction and LC-MS-MS Analysis. J Anal Toxicol. 2021.
Cox, J., Mathison, K., Ott, C., DelTondo, J., Kraner, J. C., DeCaprio, A. P., & Arroyo-Mora, L. E. (2021). Quantitation and Validation of 34 Fentanyl Analogs from Liver Tissue Using a QuEChERS Extraction and LC-MS-MS Analysis. Journal of Analytical Toxicology. https://doi.org/10.1093/jat/bkab009
Cox J, et al. Quantitation and Validation of 34 Fentanyl Analogs From Liver Tissue Using a QuEChERS Extraction and LC-MS-MS Analysis. J Anal Toxicol. 2021 Jan 30; PubMed PMID: 33515247.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Quantitation and Validation of 34 Fentanyl Analogs from Liver Tissue Using a QuEChERS Extraction and LC-MS-MS Analysis. AU - Cox,Joseph, AU - Mathison,Kylea, AU - Ott,Colby, AU - DelTondo,Joseph, AU - Kraner,James C, AU - DeCaprio,Anthony P, AU - Arroyo-Mora,Luis E, Y1 - 2021/01/30/ PY - 2020/10/09/received PY - 2021/01/11/revised PY - 2021/01/18/accepted PY - 2021/1/30/entrez PY - 2021/1/31/pubmed PY - 2021/1/31/medline JF - Journal of analytical toxicology JO - J Anal Toxicol N2 - Since 2013, drug overdose deaths involving synthetic opioids (including fentanyl and fentanyl analogs) have increased from 3,105 to 31,335 in 2018. Postmortem toxicological analysis in fentanyl-related overdose deaths is complicated by the high potency of the drug, often resulting in low analyte concentrations and associations with toxicity, multidrug use, novelty of emerging fentanyl analogs and postmortem redistribution. Objectives for this study include the development of a quick, easy, cheap, effective, rugged and safe (QuEChERS) extraction and subsequent liquid chromatography-mass spectrometry/mass spectrometry analysis, validation of the method following the American Academy of Forensic Sciences Standards Board (ASB) standard 036 requirements and application to authentic liver specimens for 34 analytes including fentanyl, metabolites and fentanyl analogs. The bias for all 34 fentanyl analogs did not exceed ±10% for any of the low, medium or high concentrations and the %CV did not exceed 20%. No interferences were identified. All 34 analytes were within the criteria for acceptable percent ionization suppression or enhancement with the low concentration ranging from -10.2% to 23.7% and the high concentration ranging from -7.1% to 11.0%. Liver specimens from 22 authentic postmortem cases were extracted and analyzed with all samples being positive for at least one target analyte from the 34 compounds. Of the 22 samples, 17 contained fentanyl and metabolites plus at least one fentanyl analog. The highest concentration for a fentanyl analog was 541.4 μg/kg of para-fluoroisobutyryl fentanyl (FIBF). The concentrations for fentanyl (n = 20) ranged between 3.6 and 164.9 μg/kg with a mean of 54.7 μg/kg. The fentanyl analog that was most encountered was methoxyacetyl fentanyl (n = 11) with a range of 0.2-4.6 μg/kg and a mean of 1.3 μg/kg. The QuEChERS extraction was fully validated using the ASB Standard 036 requirements for fentanyl, metabolites and fentanyl analogs in liver tissue. SN - 1945-2403 UR - https://www.unboundmedicine.com/medline/citation/33515247/Quantitation_and_Validation_of_34_Fentanyl_Analogs_from_Liver_Tissue_Using_a_QuEChERS_Extraction_and_LC_MS_MS_Analysis_ L2 - https://academic.oup.com/jat/article-lookup/doi/10.1093/jat/bkab009 DB - PRIME DP - Unbound Medicine ER -
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