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Summary of the Available Molecular Methods for Detection of SARS-CoV-2 during the Ongoing Pandemic.
Int J Mol Sci. 2021 Jan 28; 22(3)IJ

Abstract

Since early 2020, the COVID-19 pandemic has caused an excess in morbidity and mortality rates worldwide. Containment strategies rely firstly on rapid and sensitive laboratory diagnosis, with molecular detection of the viral genome in respiratory samples being the gold standard. The reliability of diagnostic protocols could be affected by SARS-CoV-2 genetic variability. In fact, mutations occurring during SARS-CoV-2 genomic evolution can involve the regions targeted by the diagnostic probes. Following a review of the literature and an in silico analysis of the most recently described virus variants (including the UK B 1.1.7 and the South Africa 501Y.V2 variants), we conclude that the described genetic variability should have minimal or no effect on the sensitivity of existing diagnostic protocols for SARS-CoV-2 genome detection. However, given the continuous emergence of new variants, the situation should be monitored in the future, and protocols including multiple targets should be preferred.

Authors+Show Affiliations

Department of Clinical and Experimental Medicine, University of Foggia, 71122 Foggia, Italy. IRCCS Don Carlo Gnocchi Foundation, 50143 Florence, Italy.Department of Experimental and Clinical Medicine, University of Florence, 50134 Florence, Italy. Clinical Microbiology and Virology Unit, Florence Careggi University Hospital, 50134 Florence, Italy.Department of Experimental and Clinical Medicine, University of Florence, 50134 Florence, Italy. Clinical Microbiology and Virology Unit, Florence Careggi University Hospital, 50134 Florence, Italy.Department of Clinical and Experimental Medicine, University of Foggia, 71122 Foggia, Italy.

Pub Type(s)

Journal Article
Review

Language

eng

PubMed ID

33525651

Citation

Arena, Fabio, et al. "Summary of the Available Molecular Methods for Detection of SARS-CoV-2 During the Ongoing Pandemic." International Journal of Molecular Sciences, vol. 22, no. 3, 2021.
Arena F, Pollini S, Rossolini GM, et al. Summary of the Available Molecular Methods for Detection of SARS-CoV-2 during the Ongoing Pandemic. Int J Mol Sci. 2021;22(3).
Arena, F., Pollini, S., Rossolini, G. M., & Margaglione, M. (2021). Summary of the Available Molecular Methods for Detection of SARS-CoV-2 during the Ongoing Pandemic. International Journal of Molecular Sciences, 22(3). https://doi.org/10.3390/ijms22031298
Arena F, et al. Summary of the Available Molecular Methods for Detection of SARS-CoV-2 During the Ongoing Pandemic. Int J Mol Sci. 2021 Jan 28;22(3) PubMed PMID: 33525651.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Summary of the Available Molecular Methods for Detection of SARS-CoV-2 during the Ongoing Pandemic. AU - Arena,Fabio, AU - Pollini,Simona, AU - Rossolini,Gian Maria, AU - Margaglione,Maurizio, Y1 - 2021/01/28/ PY - 2021/01/12/received PY - 2021/01/24/revised PY - 2021/01/25/accepted PY - 2021/2/2/entrez PY - 2021/2/3/pubmed PY - 2021/2/13/medline KW - 501Y.V2 variant KW - B.1.1.7 variant KW - COVID-19 KW - Coronaviridae KW - UK variant KW - diagnosis KW - molecular methods KW - pandemic KW - virus JF - International journal of molecular sciences JO - Int J Mol Sci VL - 22 IS - 3 N2 - Since early 2020, the COVID-19 pandemic has caused an excess in morbidity and mortality rates worldwide. Containment strategies rely firstly on rapid and sensitive laboratory diagnosis, with molecular detection of the viral genome in respiratory samples being the gold standard. The reliability of diagnostic protocols could be affected by SARS-CoV-2 genetic variability. In fact, mutations occurring during SARS-CoV-2 genomic evolution can involve the regions targeted by the diagnostic probes. Following a review of the literature and an in silico analysis of the most recently described virus variants (including the UK B 1.1.7 and the South Africa 501Y.V2 variants), we conclude that the described genetic variability should have minimal or no effect on the sensitivity of existing diagnostic protocols for SARS-CoV-2 genome detection. However, given the continuous emergence of new variants, the situation should be monitored in the future, and protocols including multiple targets should be preferred. SN - 1422-0067 UR - https://www.unboundmedicine.com/medline/citation/33525651/Summary_of_the_Available_Molecular_Methods_for_Detection_of_SARS_CoV_2_during_the_Ongoing_Pandemic_ L2 - https://www.mdpi.com/resolver?pii=ijms22031298 DB - PRIME DP - Unbound Medicine ER -