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Effects of culture conditions on susceptibility of alveolar epithelial cell monolayers to NO2.
Toxicol Lett 1988; 40(3):247-55TL

Abstract

The effects of nitrogen dioxide (NO2) exposure on primary cultured monolayers of rat type II pneumocytes were investigated as a function of the isolation and culture conditions. Monolayers were cultured in Eagle's minimum essential medium (MEM) and in MEM supplemented with Ham's F-12; in some experiments, the initial cell suspension was also replated after 3 h. Both supplementation of the basal medium and replating increased the sensitivity of the monolayers to NO2, as measured by reduction in dome formation of plastic dishes 24 h post-exposure. These findings suggest that comparisons of in vitro toxicologic observations may be complicated by the effects of specific experimental conditions.

Authors+Show Affiliations

School of Public Health, University of California, Los Angeles.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

3354009

Citation

Cheek, J M., et al. "Effects of Culture Conditions On Susceptibility of Alveolar Epithelial Cell Monolayers to NO2." Toxicology Letters, vol. 40, no. 3, 1988, pp. 247-55.
Cheek JM, Postlethwait EM, Crandall ED. Effects of culture conditions on susceptibility of alveolar epithelial cell monolayers to NO2. Toxicol Lett. 1988;40(3):247-55.
Cheek, J. M., Postlethwait, E. M., & Crandall, E. D. (1988). Effects of culture conditions on susceptibility of alveolar epithelial cell monolayers to NO2. Toxicology Letters, 40(3), pp. 247-55.
Cheek JM, Postlethwait EM, Crandall ED. Effects of Culture Conditions On Susceptibility of Alveolar Epithelial Cell Monolayers to NO2. Toxicol Lett. 1988;40(3):247-55. PubMed PMID: 3354009.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Effects of culture conditions on susceptibility of alveolar epithelial cell monolayers to NO2. AU - Cheek,J M, AU - Postlethwait,E M, AU - Crandall,E D, PY - 1988/3/1/pubmed PY - 1988/3/1/medline PY - 1988/3/1/entrez SP - 247 EP - 55 JF - Toxicology letters JO - Toxicol. Lett. VL - 40 IS - 3 N2 - The effects of nitrogen dioxide (NO2) exposure on primary cultured monolayers of rat type II pneumocytes were investigated as a function of the isolation and culture conditions. Monolayers were cultured in Eagle's minimum essential medium (MEM) and in MEM supplemented with Ham's F-12; in some experiments, the initial cell suspension was also replated after 3 h. Both supplementation of the basal medium and replating increased the sensitivity of the monolayers to NO2, as measured by reduction in dome formation of plastic dishes 24 h post-exposure. These findings suggest that comparisons of in vitro toxicologic observations may be complicated by the effects of specific experimental conditions. SN - 0378-4274 UR - https://www.unboundmedicine.com/medline/citation/3354009/Effects_of_culture_conditions_on_susceptibility_of_alveolar_epithelial_cell_monolayers_to_NO2_ L2 - https://linkinghub.elsevier.com/retrieve/pii/0378-4274(88)90048-3 DB - PRIME DP - Unbound Medicine ER -