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The Influence of Hydrolytic Enzymes on Tannin Adsorption-Desorption onto Grape Cell Walls in a Wine-Like Matrix.
Molecules. 2021 Feb 02; 26(3)M

Abstract

This study evaluates the capacity of four hydrolytic enzymes to limit the interactions between grape cell-walls and tannins and/or to favor tannin desorption. Adsorption and desorption tests were conducted by mixing a commercial seed tannin with purified skin cell-walls from Syrah grapes, in the presence or absence of hydrolytic enzymes, in a model-wine solution. The effects of the enzymes were evaluated by measuring the tannins in solution by High Performance Liquid Chromatography (HPLC) and the changes in the cell wall polysaccharide network by Comprehensive Microarray Polymer Profiling (COMPP) while the polysaccharides liberated from cell walls were analyzed by Size Exclusion Chromatography (SEC). The results showed that the enzymes limited the interaction between tannins and cell walls, especially cellulase, pectinase and xylanase, an effect associated with the cell wall structural modifications caused by the enzymes, which reduced their capacity to bind tannins. With regards to the tannin desorption process, enzymes did not play a significant role in liberating bound tannins. Those enzymes that showed the highest effect in limiting the adsorption of tannins and in disorganizing the cell wall structure, cellulase and pectinase, did not lead to a desorption of bound tannins, although they still showed a capacity of affecting cell wall structure. The results indicate that enzymes are not able to access those polysaccharides where tannins are bound, thus, they are not a useful tool for desorbing tannins from cell walls. The practical importance implications of these findings are discussed in the manuscript.

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Authors+Show Affiliations

Osete-Alcaraz A
Department of Food Science and Technology, Faculty of Veterinary Science, University of Murcia, Campus de Espinardo, 30100 Murcia, Spain.
Gómez-Plaza E
Department of Food Science and Technology, Faculty of Veterinary Science, University of Murcia, Campus de Espinardo, 30100 Murcia, Spain.
Martínez-Pérez P
Department of Food Science and Technology, Faculty of Veterinary Science, University of Murcia, Campus de Espinardo, 30100 Murcia, Spain.
Weiller F
Department of Viticulture and Oenology, Faculty of AgriSciences, South African Grape and Wine Research Institute, Stellenbosch University, Matieland 7602, South Africa.
Schückel J
Department of Plant and Environmental Sciences, Faculty of Science, University of Copenhagen, DK-1001 Copenhagen, Denmark. Glycospot R&D, Thorvaldsensvej 40, B102, DK-1871 Frederiksberg, Denmark.
Willatsd WGT
School of Agriculture, Food and Rural Development, Newcastle University, Newcastle-upon-Tyne NE1 4LB, UK.
Moore JP
Department of Viticulture and Oenology, Faculty of AgriSciences, South African Grape and Wine Research Institute, Stellenbosch University, Matieland 7602, South Africa.
Ros-García JM
Department of Food Science and Technology, Faculty of Veterinary Science, University of Murcia, Campus de Espinardo, 30100 Murcia, Spain.
Bautista-Ortín AB
Department of Food Science and Technology, Faculty of Veterinary Science, University of Murcia, Campus de Espinardo, 30100 Murcia, Spain.

MeSH

Cell WallEnzymesHydrolysisSeedsSolubilityTanninsVitisWine

Pub Type(s)

Journal Article

Language

eng

PubMed ID

33540867

Citation

Osete-Alcaraz, Andrea, et al. "The Influence of Hydrolytic Enzymes On Tannin Adsorption-Desorption Onto Grape Cell Walls in a Wine-Like Matrix." Molecules (Basel, Switzerland), vol. 26, no. 3, 2021.
Osete-Alcaraz A, Gómez-Plaza E, Martínez-Pérez P, et al. The Influence of Hydrolytic Enzymes on Tannin Adsorption-Desorption onto Grape Cell Walls in a Wine-Like Matrix. Molecules. 2021;26(3).
Osete-Alcaraz, A., Gómez-Plaza, E., Martínez-Pérez, P., Weiller, F., Schückel, J., Willatsd, W. G. T., Moore, J. P., Ros-García, J. M., & Bautista-Ortín, A. B. (2021). The Influence of Hydrolytic Enzymes on Tannin Adsorption-Desorption onto Grape Cell Walls in a Wine-Like Matrix. Molecules (Basel, Switzerland), 26(3). https://doi.org/10.3390/molecules26030770
Osete-Alcaraz A, et al. The Influence of Hydrolytic Enzymes On Tannin Adsorption-Desorption Onto Grape Cell Walls in a Wine-Like Matrix. Molecules. 2021 Feb 2;26(3) PubMed PMID: 33540867.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The Influence of Hydrolytic Enzymes on Tannin Adsorption-Desorption onto Grape Cell Walls in a Wine-Like Matrix. AU - Osete-Alcaraz,Andrea, AU - Gómez-Plaza,Encarna, AU - Martínez-Pérez,Pilar, AU - Weiller,Florent, AU - Schückel,Julia, AU - Willatsd,William G T, AU - Moore,John P, AU - Ros-García,José M, AU - Bautista-Ortín,Ana B, Y1 - 2021/02/02/ PY - 2021/01/05/received PY - 2021/01/25/revised PY - 2021/01/29/accepted PY - 2021/2/5/entrez PY - 2021/2/6/pubmed PY - 2021/5/11/medline KW - cell-wall KW - grape KW - hydrolytic enzymes KW - polysaccharide KW - tannin JF - Molecules (Basel, Switzerland) JO - Molecules VL - 26 IS - 3 N2 - This study evaluates the capacity of four hydrolytic enzymes to limit the interactions between grape cell-walls and tannins and/or to favor tannin desorption. Adsorption and desorption tests were conducted by mixing a commercial seed tannin with purified skin cell-walls from Syrah grapes, in the presence or absence of hydrolytic enzymes, in a model-wine solution. The effects of the enzymes were evaluated by measuring the tannins in solution by High Performance Liquid Chromatography (HPLC) and the changes in the cell wall polysaccharide network by Comprehensive Microarray Polymer Profiling (COMPP) while the polysaccharides liberated from cell walls were analyzed by Size Exclusion Chromatography (SEC). The results showed that the enzymes limited the interaction between tannins and cell walls, especially cellulase, pectinase and xylanase, an effect associated with the cell wall structural modifications caused by the enzymes, which reduced their capacity to bind tannins. With regards to the tannin desorption process, enzymes did not play a significant role in liberating bound tannins. Those enzymes that showed the highest effect in limiting the adsorption of tannins and in disorganizing the cell wall structure, cellulase and pectinase, did not lead to a desorption of bound tannins, although they still showed a capacity of affecting cell wall structure. The results indicate that enzymes are not able to access those polysaccharides where tannins are bound, thus, they are not a useful tool for desorbing tannins from cell walls. The practical importance implications of these findings are discussed in the manuscript. SN - 1420-3049 UR - https://www.unboundmedicine.com/medline/citation/33540867/The_Influence_of_Hydrolytic_Enzymes_on_Tannin_Adsorption_Desorption_onto_Grape_Cell_Walls_in_a_Wine_Like_Matrix_ DB - PRIME DP - Unbound Medicine ER -