Tags

Type your tag names separated by a space and hit enter

Structure and binding properties of Pangolin-CoV spike glycoprotein inform the evolution of SARS-CoV-2.
Nat Commun. 2021 02 05; 12(1):837.NC

Abstract

Coronaviruses of bats and pangolins have been implicated in the origin and evolution of the pandemic SARS-CoV-2. We show that spikes from Guangdong Pangolin-CoVs, closely related to SARS-CoV-2, bind strongly to human and pangolin ACE2 receptors. We also report the cryo-EM structure of a Pangolin-CoV spike protein and show it adopts a fully-closed conformation and that, aside from the Receptor-Binding Domain, it resembles the spike of a bat coronavirus RaTG13 more than that of SARS-CoV-2.

Authors+Show Affiliations

Structural Biology of Disease Processes Laboratory, Francis Crick Institute, NW1 1AT, London, UK. antoni.wrobel@crick.ac.uk.Structural Biology of Disease Processes Laboratory, Francis Crick Institute, NW1 1AT, London, UK. donald.benton@crick.ac.uk.Structural Biology of Disease Processes Laboratory, Francis Crick Institute, NW1 1AT, London, UK. Precision Medicine Center, The Seventh Affiliated Hospital, Sun Yat-sen University, Shenzhen, Guangdong, China.Structural Biology of Cells and Viruses Laboratory, Francis Crick Institute, NW1 1AT, London, UK.Structural Biology Science Technology Platform, Francis Crick Institute, NW1 1AT, London, UK.Structural Biology Science Technology Platform, Francis Crick Institute, NW1 1AT, London, UK.Structural Biology of Disease Processes Laboratory, Francis Crick Institute, NW1 1AT, London, UK.Structural Biology of Cells and Viruses Laboratory, Francis Crick Institute, NW1 1AT, London, UK.Structural Biology of Disease Processes Laboratory, Francis Crick Institute, NW1 1AT, London, UK.Structural Biology of Disease Processes Laboratory, Francis Crick Institute, NW1 1AT, London, UK. steve.gamblin@crick.ac.uk.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

33547281

Citation

Wrobel, Antoni G., et al. "Structure and Binding Properties of Pangolin-CoV Spike Glycoprotein Inform the Evolution of SARS-CoV-2." Nature Communications, vol. 12, no. 1, 2021, p. 837.
Wrobel AG, Benton DJ, Xu P, et al. Structure and binding properties of Pangolin-CoV spike glycoprotein inform the evolution of SARS-CoV-2. Nat Commun. 2021;12(1):837.
Wrobel, A. G., Benton, D. J., Xu, P., Calder, L. J., Borg, A., Roustan, C., Martin, S. R., Rosenthal, P. B., Skehel, J. J., & Gamblin, S. J. (2021). Structure and binding properties of Pangolin-CoV spike glycoprotein inform the evolution of SARS-CoV-2. Nature Communications, 12(1), 837. https://doi.org/10.1038/s41467-021-21006-9
Wrobel AG, et al. Structure and Binding Properties of Pangolin-CoV Spike Glycoprotein Inform the Evolution of SARS-CoV-2. Nat Commun. 2021 02 5;12(1):837. PubMed PMID: 33547281.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Structure and binding properties of Pangolin-CoV spike glycoprotein inform the evolution of SARS-CoV-2. AU - Wrobel,Antoni G, AU - Benton,Donald J, AU - Xu,Pengqi, AU - Calder,Lesley J, AU - Borg,Annabel, AU - Roustan,Chloë, AU - Martin,Stephen R, AU - Rosenthal,Peter B, AU - Skehel,John J, AU - Gamblin,Steven J, Y1 - 2021/02/05/ PY - 2020/08/11/received PY - 2021/01/04/accepted PY - 2021/2/6/entrez PY - 2021/2/7/pubmed PY - 2021/2/23/medline SP - 837 EP - 837 JF - Nature communications JO - Nat Commun VL - 12 IS - 1 N2 - Coronaviruses of bats and pangolins have been implicated in the origin and evolution of the pandemic SARS-CoV-2. We show that spikes from Guangdong Pangolin-CoVs, closely related to SARS-CoV-2, bind strongly to human and pangolin ACE2 receptors. We also report the cryo-EM structure of a Pangolin-CoV spike protein and show it adopts a fully-closed conformation and that, aside from the Receptor-Binding Domain, it resembles the spike of a bat coronavirus RaTG13 more than that of SARS-CoV-2. SN - 2041-1723 UR - https://www.unboundmedicine.com/medline/citation/33547281/Structure_and_binding_properties_of_Pangolin_CoV_spike_glycoprotein_inform_the_evolution_of_SARS_CoV_2_ L2 - https://doi.org/10.1038/s41467-021-21006-9 DB - PRIME DP - Unbound Medicine ER -