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Fast and cost-effective screening for SARS-CoV-2 variants in a routine diagnostic setting.
Dent Mater. 2021 03; 37(3):e95-e97.DM

Abstract

OBJECTIVE

The aim is to recommend a fast and cost-effective screening procedure for UK/SA SARS-CoV-2 variants in a routing diagnostic setting.

METHODS

A rapid procedure using qPCR is described to provide clinicians with information about the two currently most prevalent variants (B1.1.7 and B1.351) that harbour receptor binding domain mutation N501Y. The N501Y specific assay only delivers an amplification signal if the Y501 variant is present.

RESULTS

436 samples initially screened positive for SARS-CoV-2 were randomly selected. Only one of these samples showed a fluorescence signal increase indicative for the Y501 variant. The remaining 435 samples had a melting peak at 54 °C indicating the N501 wildtype.

SIGNIFICANCE

The screening of a broad population base can still be performed with the established test system. In case of a positive test for SARS-CoV-2 and corresponding clinical and anamnestic indications, a second qPCR for the mutation N501Y can follow and deliver the result to public health authorities and to the treating physician within a few hours.

Authors+Show Affiliations

Laboratory Becker & Colleagues, Führichstr. 70, 81671 Munich, Germany; Department of Operative/Restorative Dentistry, Periodontology and Pedodontics, Ludwig-Maximilians-Universität München, Goethestr. 70, 80336 Munich, Germany. Electronic address: juergen.durner@med.uni-muenchen.de.Laboratory Becker & Colleagues, Führichstr. 70, 81671 Munich, Germany.Laboratory Becker & Colleagues, Führichstr. 70, 81671 Munich, Germany.Laboratory Becker & Colleagues, Führichstr. 70, 81671 Munich, Germany.School of Medical Sciences and Photon Science Institute, University of Manchester, UK.Laboratory Becker & Colleagues, Führichstr. 70, 81671 Munich, Germany; Department of Operative/Restorative Dentistry, Periodontology and Pedodontics, Ludwig-Maximilians-Universität München, Goethestr. 70, 80336 Munich, Germany.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

33551188

Citation

Durner, Jürgen, et al. "Fast and Cost-effective Screening for SARS-CoV-2 Variants in a Routine Diagnostic Setting." Dental Materials : Official Publication of the Academy of Dental Materials, vol. 37, no. 3, 2021, pp. e95-e97.
Durner J, Burggraf S, Czibere L, et al. Fast and cost-effective screening for SARS-CoV-2 variants in a routine diagnostic setting. Dent Mater. 2021;37(3):e95-e97.
Durner, J., Burggraf, S., Czibere, L., Tehrani, A., Watts, D. C., & Becker, M. (2021). Fast and cost-effective screening for SARS-CoV-2 variants in a routine diagnostic setting. Dental Materials : Official Publication of the Academy of Dental Materials, 37(3), e95-e97. https://doi.org/10.1016/j.dental.2021.01.015
Durner J, et al. Fast and Cost-effective Screening for SARS-CoV-2 Variants in a Routine Diagnostic Setting. Dent Mater. 2021;37(3):e95-e97. PubMed PMID: 33551188.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Fast and cost-effective screening for SARS-CoV-2 variants in a routine diagnostic setting. AU - Durner,Jürgen, AU - Burggraf,Siegfried, AU - Czibere,Ludwig, AU - Tehrani,Arman, AU - Watts,David C, AU - Becker,Marc, Y1 - 2021/01/29/ PY - 2021/01/18/received PY - 2021/01/19/accepted PY - 2021/2/9/pubmed PY - 2021/3/4/medline PY - 2021/2/8/entrez KW - N501Y KW - PCR KW - SARS-CoV-2 mutations KW - South Africa KW - United Kingdom SP - e95 EP - e97 JF - Dental materials : official publication of the Academy of Dental Materials JO - Dent Mater VL - 37 IS - 3 N2 - OBJECTIVE: The aim is to recommend a fast and cost-effective screening procedure for UK/SA SARS-CoV-2 variants in a routing diagnostic setting. METHODS: A rapid procedure using qPCR is described to provide clinicians with information about the two currently most prevalent variants (B1.1.7 and B1.351) that harbour receptor binding domain mutation N501Y. The N501Y specific assay only delivers an amplification signal if the Y501 variant is present. RESULTS: 436 samples initially screened positive for SARS-CoV-2 were randomly selected. Only one of these samples showed a fluorescence signal increase indicative for the Y501 variant. The remaining 435 samples had a melting peak at 54 °C indicating the N501 wildtype. SIGNIFICANCE: The screening of a broad population base can still be performed with the established test system. In case of a positive test for SARS-CoV-2 and corresponding clinical and anamnestic indications, a second qPCR for the mutation N501Y can follow and deliver the result to public health authorities and to the treating physician within a few hours. SN - 1879-0097 UR - https://www.unboundmedicine.com/medline/citation/33551188/Fast_and_cost_effective_screening_for_SARS_CoV_2_variants_in_a_routine_diagnostic_setting_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0109-5641(21)00035-X DB - PRIME DP - Unbound Medicine ER -