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[Effect of electroacupuncture on insulin sensitivity and TLR4/ IκBα/ NF-κB signaling in hypothalamus of obese rats with insulin resistance].
Zhen Ci Yan Jiu. 2021 Jan 25; 46(1):33-8.ZC

Abstract

OBJECTIVE

To observe the effects of electroacupuncture on the hypothalamic Toll-like receptor 4 (TLR4)/ inhibitor nuclear factor kappa-B α(IκBα)/nuclear factor-κB (NF-κB) signaling pathway in obese insulin resistance (OIR) rats,so as to explore the mechanism of EA underlying improving of insulin resistance.

METHODS

Rats were randomly divided into normal, model and EA groups, with 8 rats in each group. The rat model of OIR was established by feeding with high-fat diet for 8 weeks. EA(2 Hz,1 mA)was applied to unilateral"Zusanli"(ST36),"Fenglong"(ST40),"Zhongwan"(CV12)and"Guanyuan"(CV4)for 10 min, 3 times a week for 8 weeks. The body mass, fasting blood glucose(FBG) and postprandial blood glucose (PBG) were measured before and after 2、4、6、8 weeks' intervention. An intraperitoneal injection glucose tolerance test and hyperglycemic clamps were applied to test insulin resistance. The expression of TLR4、p-IκBα、NF-κB p65、TNF-α、IL-1β mRNA and protein in hypothalamus was detected by quantitative real-time PCR and Western blot, separately.

RESULTS

Compared with the normal group, the body mass and PBG of the model group were significantly increased (P<0.01); glucose infusion rate(GIR) was significantly reduced (P<0.01); in the IPGTT test, the increase in blood glucose was significantly greater after 90 and 120 min of glucose injection(P<0.01); the hypothalamus TLR4, NF-κB p65,p-IκBα, TNF-α, IL-1β mRNA and protein expressions were all significantly increased (P<0.01). After EA intervention, the body weight and PBG were significantly down-regulated after 6 weeks and 2 weeks of intervention (P<0.05, P<0.01); GIR were significantly up-regulated after 8 weeks of intervention (P<0.05); In the IPGTT test, the increase in blood glucose 60 min after glucose injection was significantly down-regulated (P<0.05); hypothalamus TLR4, NF-κB p65,p-IκBα, TNF-α, IL -1β mRNA and protein expression were significantly down-regulated (P<0.01).

CONCLUSION

EA can reduce the body weight of OIR rats and improve IR, which may be related to down-regulating the hypothalamic TLR4/IκBα/NF-κB signaling pathway.

Authors+Show Affiliations

Department of Rehabilitation, Wuhan Third Hospital, Wuhan 430065, China.College of Acupuncture-moxibustion and Orthopedics, Hubei University of Chinese Medicine, Wuhan 430061.Department of Rehabilitation, Wuhan Third Hospital, Wuhan 430065, China.College of Acupuncture-moxibustion and Orthopedics, Hubei University of Chinese Medicine, Wuhan 430061.College of Acupuncture-moxibustion and Orthopedics, Hubei University of Chinese Medicine, Wuhan 430061.Department of Children Encephalopathy Rehabilitation, The Third People's Hospital in Hubei Province, Wuhan 430033.

Pub Type(s)

Journal Article

Language

chi

PubMed ID

33559423

Citation

Wu, Huan, et al. "[Effect of Electroacupuncture On Insulin Sensitivity and TLR4/ IκBα/ NF-κB Signaling in Hypothalamus of Obese Rats With Insulin Resistance]." Zhen Ci Yan Jiu = Acupuncture Research, vol. 46, no. 1, 2021, pp. 33-8.
Wu H, Chen L, Zhang ZQ, et al. [Effect of electroacupuncture on insulin sensitivity and TLR4/ IκBα/ NF-κB signaling in hypothalamus of obese rats with insulin resistance]. Zhen Ci Yan Jiu. 2021;46(1):33-8.
Wu, H., Chen, L., Zhang, Z. Q., Chen, B. G., Liang, F. X., & Lin, W. (2021). [Effect of electroacupuncture on insulin sensitivity and TLR4/ IκBα/ NF-κB signaling in hypothalamus of obese rats with insulin resistance]. Zhen Ci Yan Jiu = Acupuncture Research, 46(1), 33-8. https://doi.org/10.13702/j.1000-0607.200324
Wu H, et al. [Effect of Electroacupuncture On Insulin Sensitivity and TLR4/ IκBα/ NF-κB Signaling in Hypothalamus of Obese Rats With Insulin Resistance]. Zhen Ci Yan Jiu. 2021 Jan 25;46(1):33-8. PubMed PMID: 33559423.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Effect of electroacupuncture on insulin sensitivity and TLR4/ IκBα/ NF-κB signaling in hypothalamus of obese rats with insulin resistance]. AU - Wu,Huan, AU - Chen,Li, AU - Zhang,Zhao-Qing, AU - Chen,Bang-Guo, AU - Liang,Feng-Xia, AU - Lin,Wei, PY - 2021/2/9/entrez PY - 2021/2/10/pubmed PY - 2021/2/11/medline KW - Electroacupuncture KW - Inhibitor nuclear factor kappa-Bα KW - Insulin resistance KW - Nuclear factor-κB KW - Obesity KW - Toll-like receptor 4 SP - 33 EP - 8 JF - Zhen ci yan jiu = Acupuncture research JO - Zhen Ci Yan Jiu VL - 46 IS - 1 N2 - OBJECTIVE: To observe the effects of electroacupuncture on the hypothalamic Toll-like receptor 4 (TLR4)/ inhibitor nuclear factor kappa-B α(IκBα)/nuclear factor-κB (NF-κB) signaling pathway in obese insulin resistance (OIR) rats,so as to explore the mechanism of EA underlying improving of insulin resistance. METHODS: Rats were randomly divided into normal, model and EA groups, with 8 rats in each group. The rat model of OIR was established by feeding with high-fat diet for 8 weeks. EA(2 Hz,1 mA)was applied to unilateral"Zusanli"(ST36),"Fenglong"(ST40),"Zhongwan"(CV12)and"Guanyuan"(CV4)for 10 min, 3 times a week for 8 weeks. The body mass, fasting blood glucose(FBG) and postprandial blood glucose (PBG) were measured before and after 2、4、6、8 weeks' intervention. An intraperitoneal injection glucose tolerance test and hyperglycemic clamps were applied to test insulin resistance. The expression of TLR4、p-IκBα、NF-κB p65、TNF-α、IL-1β mRNA and protein in hypothalamus was detected by quantitative real-time PCR and Western blot, separately. RESULTS: Compared with the normal group, the body mass and PBG of the model group were significantly increased (P<0.01); glucose infusion rate(GIR) was significantly reduced (P<0.01); in the IPGTT test, the increase in blood glucose was significantly greater after 90 and 120 min of glucose injection(P<0.01); the hypothalamus TLR4, NF-κB p65,p-IκBα, TNF-α, IL-1β mRNA and protein expressions were all significantly increased (P<0.01). After EA intervention, the body weight and PBG were significantly down-regulated after 6 weeks and 2 weeks of intervention (P<0.05, P<0.01); GIR were significantly up-regulated after 8 weeks of intervention (P<0.05); In the IPGTT test, the increase in blood glucose 60 min after glucose injection was significantly down-regulated (P<0.05); hypothalamus TLR4, NF-κB p65,p-IκBα, TNF-α, IL -1β mRNA and protein expression were significantly down-regulated (P<0.01). CONCLUSION: EA can reduce the body weight of OIR rats and improve IR, which may be related to down-regulating the hypothalamic TLR4/IκBα/NF-κB signaling pathway. SN - 1000-0607 UR - https://www.unboundmedicine.com/medline/citation/33559423/[Effect_of_electroacupuncture_on_insulin_sensitivity_and_TLR4/_IκBα/_NF_κB_signaling_in_hypothalamus_of_obese_rats_with_insulin_resistance]_ DB - PRIME DP - Unbound Medicine ER -