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[Effect of electroacupuncture pretreatment on transient receptor potential vanilloid 1(TRPV1)/calcitonin gene-related peptide(CGRP) signal and NF-κB p65 protein expression in rats with acute myocardial ischemia].
Zhen Ci Yan Jiu. 2021 Jan 25; 46(1):58-63.ZC

Abstract

OBJECTIVE

To observe the effect of electroacupuncture(EA)pretreatment on transient receptor potential vanilloid 1(TRPV1)/calcitonin gene-related peptide(CGRP)signal and nuclear factor-κB p65 (NF-κB p65) protein expression in myocardial tissue of acute myocardial ischemic injury (AMI) rats, and to investigate the possible mechanism of electroacupuncture pretreatment against AMI.

METHODS

A total of 60 adult male SD rats were randomly divided into blank control, sham operation, model and EA pretreatment groups, 15 rats in each group. The acute myocardial ischemia model was established by ligating the left anterior descending (LAD)branch of the coronary artery in the model group and EA pretreatment group, while threading but no ligating at left anterior descending branch of the coronary artery was applied in the sham operation group. In the EA pretreatment group, bilateral "Neiguan"(PC6) acupoints were selected, with intensity of 2 mA and frequency of 2 Hz/100 Hz, for 20 min, once daily for 7 days before modeling. Electrocardiogram (ECG) was recorded by physiological signal acquisition system, and the ST segment potential offset values of standard Ⅱ lead were analyzed before surgery,30 min and 24 h after operation. The TTC staining was used to observe the percentage of myocardial infarction area. The HE staining was used to observe the pathological changes of myocardial tissue and the degree of inflammatory cell infiltration. And Western blot was used to detect TRPV1/CGRP signal and NF-κB p65 protein expression levels in myocardial tissue.

RESULTS

Compared with the sham operation group, the ECG-J point potential in the model group was significantly increased at 30 min and decreased at 24 h after operation (P<0.05), myocardial infarction area increased significantly (P<0.05), the myocardial fibers were obviously disordered, inflammatory cell infiltration was obvious, and the expressions of TRPV1,CGRP and NF-κB p65 proteins were all increased (P<0.05). Compared with the model group, the EA pretreatment group was decreased in the ECG-J point potential at 30 min after operation(P<0.05), significantly reduced in myocardial infarction area (P<0.05), improved in the morphology of myocardial fibers, reduced ininflammatory cell infiltration, and increased in the protein expressions of TRPV1 and CGRP in myocardium (P<0.05), significantly decreased in the protein expression of NF-κB p65 (P<0.05).

CONCLUSION

EA pretreatment may enhance TRPV1/CGRP signaling, down-regulate NF-κB p65 protein expression, reduce myocardial inflammatory response status, improve AMI injury, and reduce myocardial infarction area.

Authors+Show Affiliations

College of Acupuncture and Massage, Health and Rehabilitation College, Nanjing University of Chinese Medicine, Nanjing 210023, China.College of Acupuncture and Massage, Health and Rehabilitation College, Nanjing University of Chinese Medicine, Nanjing 210023, China.College of Acupuncture and Massage, Health and Rehabilitation College, Nanjing University of Chinese Medicine, Nanjing 210023, China; Key Laboratory for Researching Combination of Acupuncture and Drugs of Ministry of Education, Nanjing University of Chinese Medicine, Nanjing 210023, China.College of Acupuncture and Massage, Health and Rehabilitation College, Nanjing University of Chinese Medicine, Nanjing 210023, China.College of Acupuncture and Massage, Health and Rehabilitation College, Nanjing University of Chinese Medicine, Nanjing 210023, China.College of Acupuncture and Massage, Health and Rehabilitation College, Nanjing University of Chinese Medicine, Nanjing 210023, China.College of Acupuncture and Massage, Health and Rehabilitation College, Nanjing University of Chinese Medicine, Nanjing 210023, China.College of Acupuncture and Massage, Health and Rehabilitation College, Nanjing University of Chinese Medicine, Nanjing 210023, China.College of Acupuncture and Massage, Health and Rehabilitation College, Nanjing University of Chinese Medicine, Nanjing 210023, China.

Pub Type(s)

Journal Article

Language

chi

PubMed ID

33559427

Citation

Wu, Jia-Hong, et al. "[Effect of Electroacupuncture Pretreatment On Transient Receptor Potential Vanilloid 1(TRPV1)/calcitonin Gene-related peptide(CGRP) Signal and NF-κB P65 Protein Expression in Rats With Acute Myocardial Ischemia]." Zhen Ci Yan Jiu = Acupuncture Research, vol. 46, no. 1, 2021, pp. 58-63.
Wu JH, Sun K, Lu SF, et al. [Effect of electroacupuncture pretreatment on transient receptor potential vanilloid 1(TRPV1)/calcitonin gene-related peptide(CGRP) signal and NF-κB p65 protein expression in rats with acute myocardial ischemia]. Zhen Ci Yan Jiu. 2021;46(1):58-63.
Wu, J. H., Sun, K., Lu, S. F., Bai, H., Xu, S. L., Xia, X. F., Liu, Y. C., Gu, Y. H., & Zhang, H. R. (2021). [Effect of electroacupuncture pretreatment on transient receptor potential vanilloid 1(TRPV1)/calcitonin gene-related peptide(CGRP) signal and NF-κB p65 protein expression in rats with acute myocardial ischemia]. Zhen Ci Yan Jiu = Acupuncture Research, 46(1), 58-63. https://doi.org/10.13702/j.1000-0607.200905
Wu JH, et al. [Effect of Electroacupuncture Pretreatment On Transient Receptor Potential Vanilloid 1(TRPV1)/calcitonin Gene-related peptide(CGRP) Signal and NF-κB P65 Protein Expression in Rats With Acute Myocardial Ischemia]. Zhen Ci Yan Jiu. 2021 Jan 25;46(1):58-63. PubMed PMID: 33559427.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Effect of electroacupuncture pretreatment on transient receptor potential vanilloid 1(TRPV1)/calcitonin gene-related peptide(CGRP) signal and NF-κB p65 protein expression in rats with acute myocardial ischemia]. AU - Wu,Jia-Hong, AU - Sun,Ke, AU - Lu,Sheng-Feng, AU - Bai,Hua, AU - Xu,Sen-Lei, AU - Xia,Xue-Feng, AU - Liu,Yu-Chen, AU - Gu,Yi-Huang, AU - Zhang,Hong-Ru, PY - 2021/2/9/entrez PY - 2021/2/10/pubmed PY - 2021/2/11/medline KW - Acute myocardial ischemia;Electroacupuncture pretreatment;Transient receptor potential vanilloid 1(TRPV1)/calcitonin gene-related peptide(CGRP) KW - Nuclear factor-κB p65 (NF-κB p65) SP - 58 EP - 63 JF - Zhen ci yan jiu = Acupuncture research JO - Zhen Ci Yan Jiu VL - 46 IS - 1 N2 - OBJECTIVE: To observe the effect of electroacupuncture(EA)pretreatment on transient receptor potential vanilloid 1(TRPV1)/calcitonin gene-related peptide(CGRP)signal and nuclear factor-κB p65 (NF-κB p65) protein expression in myocardial tissue of acute myocardial ischemic injury (AMI) rats, and to investigate the possible mechanism of electroacupuncture pretreatment against AMI. METHODS: A total of 60 adult male SD rats were randomly divided into blank control, sham operation, model and EA pretreatment groups, 15 rats in each group. The acute myocardial ischemia model was established by ligating the left anterior descending (LAD)branch of the coronary artery in the model group and EA pretreatment group, while threading but no ligating at left anterior descending branch of the coronary artery was applied in the sham operation group. In the EA pretreatment group, bilateral "Neiguan"(PC6) acupoints were selected, with intensity of 2 mA and frequency of 2 Hz/100 Hz, for 20 min, once daily for 7 days before modeling. Electrocardiogram (ECG) was recorded by physiological signal acquisition system, and the ST segment potential offset values of standard Ⅱ lead were analyzed before surgery,30 min and 24 h after operation. The TTC staining was used to observe the percentage of myocardial infarction area. The HE staining was used to observe the pathological changes of myocardial tissue and the degree of inflammatory cell infiltration. And Western blot was used to detect TRPV1/CGRP signal and NF-κB p65 protein expression levels in myocardial tissue. RESULTS: Compared with the sham operation group, the ECG-J point potential in the model group was significantly increased at 30 min and decreased at 24 h after operation (P<0.05), myocardial infarction area increased significantly (P<0.05), the myocardial fibers were obviously disordered, inflammatory cell infiltration was obvious, and the expressions of TRPV1,CGRP and NF-κB p65 proteins were all increased (P<0.05). Compared with the model group, the EA pretreatment group was decreased in the ECG-J point potential at 30 min after operation(P<0.05), significantly reduced in myocardial infarction area (P<0.05), improved in the morphology of myocardial fibers, reduced ininflammatory cell infiltration, and increased in the protein expressions of TRPV1 and CGRP in myocardium (P<0.05), significantly decreased in the protein expression of NF-κB p65 (P<0.05). CONCLUSION: EA pretreatment may enhance TRPV1/CGRP signaling, down-regulate NF-κB p65 protein expression, reduce myocardial inflammatory response status, improve AMI injury, and reduce myocardial infarction area. SN - 1000-0607 UR - https://www.unboundmedicine.com/medline/citation/33559427/[Effect_of_electroacupuncture_pretreatment_on_transient_receptor_potential_vanilloid_1_TRPV1_/calcitonin_gene_related_peptide_CGRP__signal_and_NF_κB_p65_protein_expression_in_rats_with_acute_myocardial_ischemia]_ L2 - https://antibodies.cancer.gov/detail/CPTC-S100A12-1 DB - PRIME DP - Unbound Medicine ER -