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Tegumental glucose permeability in male and female Schistosoma mansoni.
J Parasitol 1988; 74(1):116-28JP

Abstract

Tegumental hexose transporters have been kinetically characterized in mated and separated male and female Schistosoma mansoni 8-12 wk postinfection. Significant gender-specific differences in Km and Vmax were observed. In mated males, the estimated constants (mean +/- SE) were: Km = 0.63 +/- 0.31 mM, Vmax = 0.93 +/- 0.44 nmol/mg worm water/min, and the Kd = 0.25 +/- 0.09 microliter/mg worm water/min. In mated females the kinetics were: Km = 0.99 +/- 0.40 mM, Vmax = 1.22 +/- 0.42 nmol/mg worm water/min, and Kd = 0.60 +/- 0.14 microliter/mg worm water/min. The influx of 2-deoxy-D-glucose and 3-O-methylglucose has been similarly characterized; these analogs share the same glucose transporter in male and female schistosomes. 2-Deoxy-D-glucose has a higher affinity, and 3-O-methylglucose a lower affinity, than does glucose. Because mated male schistosomes supply glucose to female partners, similarities between the free glucose concentration of the male and the affinity of the transporter determined for mated female schistosomes suggest that male-to-female transfer may be a potentially rate-limiting step in glucose utilization by the female. Permeability x surface are (PS) products and Vmax/Km ratios were significantly elevated in mated schistosomes, suggesting that the transporter is primarily localized to the dorsal surface of the male. Gender- and mating-specific analyses of PS products indicate that tegumental permeability to glucose is significantly increased in mated schistosomes, and compares very favorably to that of the host liver.

Authors+Show Affiliations

Comprehensive Epilepsy Program, V. A. Wadsworth Medical Center, Los Angeles, California 90073.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, U.S. Gov't, Non-P.H.S.
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

3357096

Citation

Cornford, E M., et al. "Tegumental Glucose Permeability in Male and Female Schistosoma Mansoni." The Journal of Parasitology, vol. 74, no. 1, 1988, pp. 116-28.
Cornford EM, Fitzpatrick AM, Quirk TL, et al. Tegumental glucose permeability in male and female Schistosoma mansoni. J Parasitol. 1988;74(1):116-28.
Cornford, E. M., Fitzpatrick, A. M., Quirk, T. L., Diep, C. P., & Landaw, E. M. (1988). Tegumental glucose permeability in male and female Schistosoma mansoni. The Journal of Parasitology, 74(1), pp. 116-28.
Cornford EM, et al. Tegumental Glucose Permeability in Male and Female Schistosoma Mansoni. J Parasitol. 1988;74(1):116-28. PubMed PMID: 3357096.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Tegumental glucose permeability in male and female Schistosoma mansoni. AU - Cornford,E M, AU - Fitzpatrick,A M, AU - Quirk,T L, AU - Diep,C P, AU - Landaw,E M, PY - 1988/2/1/pubmed PY - 1988/2/1/medline PY - 1988/2/1/entrez SP - 116 EP - 28 JF - The Journal of parasitology JO - J. Parasitol. VL - 74 IS - 1 N2 - Tegumental hexose transporters have been kinetically characterized in mated and separated male and female Schistosoma mansoni 8-12 wk postinfection. Significant gender-specific differences in Km and Vmax were observed. In mated males, the estimated constants (mean +/- SE) were: Km = 0.63 +/- 0.31 mM, Vmax = 0.93 +/- 0.44 nmol/mg worm water/min, and the Kd = 0.25 +/- 0.09 microliter/mg worm water/min. In mated females the kinetics were: Km = 0.99 +/- 0.40 mM, Vmax = 1.22 +/- 0.42 nmol/mg worm water/min, and Kd = 0.60 +/- 0.14 microliter/mg worm water/min. The influx of 2-deoxy-D-glucose and 3-O-methylglucose has been similarly characterized; these analogs share the same glucose transporter in male and female schistosomes. 2-Deoxy-D-glucose has a higher affinity, and 3-O-methylglucose a lower affinity, than does glucose. Because mated male schistosomes supply glucose to female partners, similarities between the free glucose concentration of the male and the affinity of the transporter determined for mated female schistosomes suggest that male-to-female transfer may be a potentially rate-limiting step in glucose utilization by the female. Permeability x surface are (PS) products and Vmax/Km ratios were significantly elevated in mated schistosomes, suggesting that the transporter is primarily localized to the dorsal surface of the male. Gender- and mating-specific analyses of PS products indicate that tegumental permeability to glucose is significantly increased in mated schistosomes, and compares very favorably to that of the host liver. SN - 0022-3395 UR - https://www.unboundmedicine.com/medline/citation/3357096/Tegumental_glucose_permeability_in_male_and_female_Schistosoma_mansoni_ DB - PRIME DP - Unbound Medicine ER -