Tags

Type your tag names separated by a space and hit enter

Human Milk Antibodies Against S1 and S2 Subunits from SARS-CoV-2, HCoV-OC43, and HCoV-229E in Mothers with A Confirmed COVID-19 PCR, Viral SYMPTOMS, and Unexposed Mothers.
Int J Mol Sci. 2021 Feb 09; 22(4)IJ

Abstract

BACKGROUND

Preexisting immunity to SARS-CoV-2 could be related to cross-reactive antibodies to common human-coronaviruses (HCoVs). This study aimed to evaluate whether human milk antibodies against to S1 and S2 subunits SARS-CoV-2 are cross-reactive to S1 and S2 subunits HCoV-OC43 and HCoV-229E in mothers with a confirmed COVID-19 PCR test, in mothers with previous viral symptoms during COVID-19 pandemic, and in unexposed mothers; Methods: The levels of secretory IgA (SIgA)/IgA, secretory IgM (SIgM)/IgM, and IgG specific to S1 and S2 SARS-CoV-2, and reactive to S1 + S2 HCoV-OC43, and HCoV-229E were measured in milk from 7 mothers with a confirmed COVID-19 PCR test, 20 mothers with viral symptoms, and unexposed mothers (6 Ctl1-2018 and 16 Ctl2-2018) using ELISA; Results: The S2 SARS-CoV-2 IgG levels were higher in the COVID-19 PCR (p = 0.014) and viral symptom (p = 0.040) groups than in the Ctl1-2018 group. We detected a higher number of positive correlations between the antigens and secretory antibodies in the COVID-19 PCR group than in the viral symptom and Ctl-2018 groups. S1 + S2 HCoV-OC43-reactive IgG was higher in the COVID-19 group than in the control group (p = 0.002) but did not differ for the other antibodies; Conclusions: Mothers with a confirmed COVID-19 PCR and mothers with previous viral symptoms had preexisting human milk antibodies against S2 subunit SARS-CoV-2. Human milk IgG were more specific to S2 subunit SARS-CoV-2 than other antibodies, whereas SIgA and SIgM were polyreactive and cross-reactive to S1 or S2 subunit SARS-CoV-2.

Authors+Show Affiliations

Department of Neonatal Immunology and Microbiology, Medolac Laboratories A Public Benefit Corporation, Boulder City, NV 89005, USA. Department of Food Science, University of Massachusetts Amherst, Amherst, MA 01003, USA.Department of Neonatal Immunology and Microbiology, Medolac Laboratories A Public Benefit Corporation, Boulder City, NV 89005, USA.Department of Neonatal Immunology and Microbiology, Medolac Laboratories A Public Benefit Corporation, Boulder City, NV 89005, USA.Department of Food Science, University of Massachusetts Amherst, Amherst, MA 01003, USA.Medical Center, Department of Pediatrics, Pediatric Allergy/Immunology, University of Rochester, Rochester, NY 14642, USA.Medical Center, Department of Pediatrics, Pediatric Allergy/Immunology, University of Rochester, Rochester, NY 14642, USA.Department of Neonatal Immunology and Microbiology, Medolac Laboratories A Public Benefit Corporation, Boulder City, NV 89005, USA.Department of Neonatal Immunology and Microbiology, Medolac Laboratories A Public Benefit Corporation, Boulder City, NV 89005, USA.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

33572480

Citation

Demers-Mathieu, Veronique, et al. "Human Milk Antibodies Against S1 and S2 Subunits From SARS-CoV-2, HCoV-OC43, and HCoV-229E in Mothers With a Confirmed COVID-19 PCR, Viral SYMPTOMS, and Unexposed Mothers." International Journal of Molecular Sciences, vol. 22, no. 4, 2021.
Demers-Mathieu V, DaPra C, Mathijssen G, et al. Human Milk Antibodies Against S1 and S2 Subunits from SARS-CoV-2, HCoV-OC43, and HCoV-229E in Mothers with A Confirmed COVID-19 PCR, Viral SYMPTOMS, and Unexposed Mothers. Int J Mol Sci. 2021;22(4).
Demers-Mathieu, V., DaPra, C., Mathijssen, G., A Sela, D., M Jarvinen, K., Seppo, A., Fels, S., & Medo, E. (2021). Human Milk Antibodies Against S1 and S2 Subunits from SARS-CoV-2, HCoV-OC43, and HCoV-229E in Mothers with A Confirmed COVID-19 PCR, Viral SYMPTOMS, and Unexposed Mothers. International Journal of Molecular Sciences, 22(4). https://doi.org/10.3390/ijms22041749
Demers-Mathieu V, et al. Human Milk Antibodies Against S1 and S2 Subunits From SARS-CoV-2, HCoV-OC43, and HCoV-229E in Mothers With a Confirmed COVID-19 PCR, Viral SYMPTOMS, and Unexposed Mothers. Int J Mol Sci. 2021 Feb 9;22(4) PubMed PMID: 33572480.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Human Milk Antibodies Against S1 and S2 Subunits from SARS-CoV-2, HCoV-OC43, and HCoV-229E in Mothers with A Confirmed COVID-19 PCR, Viral SYMPTOMS, and Unexposed Mothers. AU - Demers-Mathieu,Veronique, AU - DaPra,Ciera, AU - Mathijssen,Gabrielle, AU - A Sela,David, AU - M Jarvinen,Kirsi, AU - Seppo,Antti, AU - Fels,Shawn, AU - Medo,Elena, Y1 - 2021/02/09/ PY - 2021/01/22/received PY - 2021/02/05/revised PY - 2021/02/07/accepted PY - 2021/2/12/entrez PY - 2021/2/13/pubmed PY - 2021/2/20/medline KW - breast milk KW - breastfeeding KW - immunoglobulins KW - infectious diseases KW - neonates KW - passive immunity KW - polyreactive antibody KW - α-coronaviruses KW - β-coronaviruses JF - International journal of molecular sciences JO - Int J Mol Sci VL - 22 IS - 4 N2 - BACKGROUND: Preexisting immunity to SARS-CoV-2 could be related to cross-reactive antibodies to common human-coronaviruses (HCoVs). This study aimed to evaluate whether human milk antibodies against to S1 and S2 subunits SARS-CoV-2 are cross-reactive to S1 and S2 subunits HCoV-OC43 and HCoV-229E in mothers with a confirmed COVID-19 PCR test, in mothers with previous viral symptoms during COVID-19 pandemic, and in unexposed mothers; Methods: The levels of secretory IgA (SIgA)/IgA, secretory IgM (SIgM)/IgM, and IgG specific to S1 and S2 SARS-CoV-2, and reactive to S1 + S2 HCoV-OC43, and HCoV-229E were measured in milk from 7 mothers with a confirmed COVID-19 PCR test, 20 mothers with viral symptoms, and unexposed mothers (6 Ctl1-2018 and 16 Ctl2-2018) using ELISA; Results: The S2 SARS-CoV-2 IgG levels were higher in the COVID-19 PCR (p = 0.014) and viral symptom (p = 0.040) groups than in the Ctl1-2018 group. We detected a higher number of positive correlations between the antigens and secretory antibodies in the COVID-19 PCR group than in the viral symptom and Ctl-2018 groups. S1 + S2 HCoV-OC43-reactive IgG was higher in the COVID-19 group than in the control group (p = 0.002) but did not differ for the other antibodies; Conclusions: Mothers with a confirmed COVID-19 PCR and mothers with previous viral symptoms had preexisting human milk antibodies against S2 subunit SARS-CoV-2. Human milk IgG were more specific to S2 subunit SARS-CoV-2 than other antibodies, whereas SIgA and SIgM were polyreactive and cross-reactive to S1 or S2 subunit SARS-CoV-2. SN - 1422-0067 UR - https://www.unboundmedicine.com/medline/citation/33572480/Human_Milk_Antibodies_Against_S1_and_S2_Subunits_from_SARS_CoV_2_HCoV_OC43_and_HCoV_229E_in_Mothers_with_A_Confirmed_COVID_19_PCR_Viral_SYMPTOMS_and_Unexposed_Mothers_ L2 - https://www.mdpi.com/resolver?pii=ijms22041749 DB - PRIME DP - Unbound Medicine ER -