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Efficacy of Euphorbia helioscopia in context to a possible connection between antioxidant and antidiabetic activities: a comparative study of different extracts.
BMC Complement Med Ther. 2021 Feb 12; 21(1):62.BC

Abstract

BACKGROUND

Euphorbia helioscopia, conventionally known as sun spurge, has been used as a traditional medicine to treat different diseases owing to its reported antitumor, antiviral and antioxidant activities.

METHODS

The current research was formulated to assess the in-vitro antioxidant and antidiabetic ability of Euphorbia helioscopia subsequent to the phytochemical analysis of its various extracts. For this purpose, methanol, ethanol and aqueous extracts were prepared using the whole dried plant. Phytochemical analysis of the extracts was done to evaluate the total flavonoid components (TFC) and total phenolic components (TPC) in the extracts. A total of seven phenolic and three flavonoid contents were documented and quantified using HPLC. Antioxidant values were found by DPPH● assay, FRAP and ABTS assays. The antidiabetic potential of the extracts was evaluated by measuring the inhibition ability of the activity of enzymes α amylase and α glucosidase.

RESULTS

After analyzing statistically, the results showed that methanolic extract possesses the highest TFC and TPC values while aqueous extract encompassed the lowest level of these contents. Invitro results showed that methanolic extract of the Euphorbia helioscopia has the maximum antioxidant capability since it showed the highest scavenging ability towards the DPPH● (IC50 value = 0.06 ± 0.02 mg/ml), FRAP (758.9 ± 25.1 μMFe+ 2/g), and ABTS (689 ± 25.94 μMTEq/g) due to the presence of high TPC (24.77 ± 0.35 mgGAEq/g) and TFC (17.95 ± 0.32 mgQEq/g) values. Antidiabetic activity in terms of inhibition potential of α amylase and α glucosidase activity was also observed maximum in methanolic extract having lowest IC50 value (0.4 ± 0.01 mg/ml and 0.45 ± 0.01 mg/ml respectively) and minimum in the aqueous extract (IC50 value = 0.57 ± 0.02 mg/ml and 0.76 ± 0.1 mg/ml respectively).

CONCLUSION

The experiment outcomes have shown that Euphorbia helioscopia extracts used in the current study contain antioxidant and antidiabetic activities; however, it is highest in its methanolic extract. The presence of the same trend towards the highest antidiabetic activity of the methanolic extract in terms of maximum inhibiting activity of α amylase and α glucosidase enzymes suggests a close association of TFC and TPC in minimizing diabetes.

Authors+Show Affiliations

Department of Physiology, Faculty of Life Sciences, Government College University, Faisalabad, Pakistan.Institute of Physiology and Pharmacology, University of Agriculture, Faisalabad, Pakistan.Department of Physiology, Faculty of Life Sciences, Government College University, Faisalabad, Pakistan.Department of Physiology, Faculty of Life Sciences, Government College University, Faisalabad, Pakistan.Department of Food Science, Faculty of Life Sciences, Government College University, Faisalabad, Pakistan.Department of Zoology, Wildlife and Fisheries, University of Agriculture, Faisalabad, Pakistan.Biomedical Research Centre, Qatar University, Doha, Qatar.Department of Physiology, Faculty of Life Sciences, Government College University, Faisalabad, Pakistan.Department of Physiology, Faculty of Life Sciences, Government College University, Faisalabad, Pakistan.Department of Physiology, Faculty of Life Sciences, Government College University, Faisalabad, Pakistan. drhaseebanwar@gcuf.edu.pk.

Pub Type(s)

Comparative Study
Journal Article

Language

eng

PubMed ID

33579270

Citation

Mustafa, Imtiaz, et al. "Efficacy of Euphorbia Helioscopia in Context to a Possible Connection Between Antioxidant and Antidiabetic Activities: a Comparative Study of Different Extracts." BMC Complementary Medicine and Therapies, vol. 21, no. 1, 2021, p. 62.
Mustafa I, Faisal MN, Hussain G, et al. Efficacy of Euphorbia helioscopia in context to a possible connection between antioxidant and antidiabetic activities: a comparative study of different extracts. BMC Complement Med Ther. 2021;21(1):62.
Mustafa, I., Faisal, M. N., Hussain, G., Muzaffar, H., Imran, M., Ijaz, M. U., Sohail, M. U., Iftikhar, A., Shaukat, A., & Anwar, H. (2021). Efficacy of Euphorbia helioscopia in context to a possible connection between antioxidant and antidiabetic activities: a comparative study of different extracts. BMC Complementary Medicine and Therapies, 21(1), 62. https://doi.org/10.1186/s12906-021-03237-x
Mustafa I, et al. Efficacy of Euphorbia Helioscopia in Context to a Possible Connection Between Antioxidant and Antidiabetic Activities: a Comparative Study of Different Extracts. BMC Complement Med Ther. 2021 Feb 12;21(1):62. PubMed PMID: 33579270.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Efficacy of Euphorbia helioscopia in context to a possible connection between antioxidant and antidiabetic activities: a comparative study of different extracts. AU - Mustafa,Imtiaz, AU - Faisal,Muhammad Naeem, AU - Hussain,Ghulam, AU - Muzaffar,Humaira, AU - Imran,Muhammad, AU - Ijaz,Muhammad Umar, AU - Sohail,Muhammad Umar, AU - Iftikhar,Arslan, AU - Shaukat,Arslan, AU - Anwar,Haseeb, Y1 - 2021/02/12/ PY - 2020/05/09/received PY - 2021/02/02/accepted PY - 2021/2/13/entrez PY - 2021/2/14/pubmed PY - 2021/9/8/medline KW - Antidiabetic KW - Antioxidant KW - Euphorbia helioscopia KW - Methanolic extract SP - 62 EP - 62 JF - BMC complementary medicine and therapies JO - BMC Complement Med Ther VL - 21 IS - 1 N2 - BACKGROUND: Euphorbia helioscopia, conventionally known as sun spurge, has been used as a traditional medicine to treat different diseases owing to its reported antitumor, antiviral and antioxidant activities. METHODS: The current research was formulated to assess the in-vitro antioxidant and antidiabetic ability of Euphorbia helioscopia subsequent to the phytochemical analysis of its various extracts. For this purpose, methanol, ethanol and aqueous extracts were prepared using the whole dried plant. Phytochemical analysis of the extracts was done to evaluate the total flavonoid components (TFC) and total phenolic components (TPC) in the extracts. A total of seven phenolic and three flavonoid contents were documented and quantified using HPLC. Antioxidant values were found by DPPH● assay, FRAP and ABTS assays. The antidiabetic potential of the extracts was evaluated by measuring the inhibition ability of the activity of enzymes α amylase and α glucosidase. RESULTS: After analyzing statistically, the results showed that methanolic extract possesses the highest TFC and TPC values while aqueous extract encompassed the lowest level of these contents. Invitro results showed that methanolic extract of the Euphorbia helioscopia has the maximum antioxidant capability since it showed the highest scavenging ability towards the DPPH● (IC50 value = 0.06 ± 0.02 mg/ml), FRAP (758.9 ± 25.1 μMFe+ 2/g), and ABTS (689 ± 25.94 μMTEq/g) due to the presence of high TPC (24.77 ± 0.35 mgGAEq/g) and TFC (17.95 ± 0.32 mgQEq/g) values. Antidiabetic activity in terms of inhibition potential of α amylase and α glucosidase activity was also observed maximum in methanolic extract having lowest IC50 value (0.4 ± 0.01 mg/ml and 0.45 ± 0.01 mg/ml respectively) and minimum in the aqueous extract (IC50 value = 0.57 ± 0.02 mg/ml and 0.76 ± 0.1 mg/ml respectively). CONCLUSION: The experiment outcomes have shown that Euphorbia helioscopia extracts used in the current study contain antioxidant and antidiabetic activities; however, it is highest in its methanolic extract. The presence of the same trend towards the highest antidiabetic activity of the methanolic extract in terms of maximum inhibiting activity of α amylase and α glucosidase enzymes suggests a close association of TFC and TPC in minimizing diabetes. SN - 2662-7671 UR - https://www.unboundmedicine.com/medline/citation/33579270/Efficacy_of_Euphorbia_helioscopia_in_context_to_a_possible_connection_between_antioxidant_and_antidiabetic_activities:_a_comparative_study_of_different_extracts_ L2 - https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/33579270/ DB - PRIME DP - Unbound Medicine ER -