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A Stable Quantitative Trait Locus on Chromosome 5BL Combined with Yr18 Conferring High-Level Adult Plant Resistance to Stripe Rust in Chinese Wheat Landrace Anyuehong.
Phytopathology. 2021 Sep; 111(9):1594-1601.P

Abstract

Chinese wheat landrace Anyuehong (AYH) has displayed high levels of stable adult plant resistance (APR) to stripe rust for >15 years. To identify quantitative trait loci (QTLs) for stripe rust resistance in AYH, a set of 110 recombinant inbred lines (RILs) was developed from a cross between AYH and susceptible cultivar Taichung 29. The parents and RILs were evaluated for final disease severity (FDS) in six field tests with a mixture of predominant Puccinia striiformis f. sp. tritici races at the adult plant stage and genotyped via the wheat 55K single-nucleotide polymorphism (SNP) array to construct a genetic map with 1,143 SNP markers. Three QTLs, designated as QYr.AYH-1AS, QYr.AYH-5BL, and QYr.AYH-7DS, were mapped on chromosome 1AS, 5BL, and 7DS, respectively. RILs combining three QTLs showed significantly lower FDS compared with the lines in other combinations. Of them, QYr.AYH-5BL and QYr.AYH-7DS were stably detected in all environments, explaining 13.6 to 21.4% and 17.6 to 33.6% of phenotypic variation, respectively. Compared with previous studies, QYr.AYH-5BL may be a new QTL, whereas QYr.AYH-7DS may be Yr18. Haplotype analysis revealed that QYr.AYH-5BL is probably present in 6.2% of the 323 surveyed Chinese wheat landraces. The kompetitive allele specific PCR (KASP) markers for QYr.AYH-5BL were developed by the linked SNP markers to successfully confirm the effects of the QTL in a validation population derived from a residual heterozygous line and were further assessed in 38 Chinese wheat landraces and 92 cultivars. Our results indicated that QYr.AYH-5BL with linked KASP markers has potential value for marker-assisted selection to improve stripe rust resistance in breeding programs.

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Authors+Show Affiliations

Long L
Triticeae Research Institute, Sichuan Agricultural University, Wenjiang, Chengdu, Sichuan, 611130, P.R. China. State Key Laboratory of Crop Gene Exploitation and Utilization in Southwest China, Wenjiang, Chengdu, Sichuan, 611130, P.R. China.
Yao F
Triticeae Research Institute, Sichuan Agricultural University, Wenjiang, Chengdu, Sichuan, 611130, P.R. China. State Key Laboratory of Crop Gene Exploitation and Utilization in Southwest China, Wenjiang, Chengdu, Sichuan, 611130, P.R. China.
Guan F
Triticeae Research Institute, Sichuan Agricultural University, Wenjiang, Chengdu, Sichuan, 611130, P.R. China. State Key Laboratory of Crop Gene Exploitation and Utilization in Southwest China, Wenjiang, Chengdu, Sichuan, 611130, P.R. China.
Cheng Y
Triticeae Research Institute, Sichuan Agricultural University, Wenjiang, Chengdu, Sichuan, 611130, P.R. China. State Key Laboratory of Crop Gene Exploitation and Utilization in Southwest China, Wenjiang, Chengdu, Sichuan, 611130, P.R. China.
Duan L
Triticeae Research Institute, Sichuan Agricultural University, Wenjiang, Chengdu, Sichuan, 611130, P.R. China. State Key Laboratory of Crop Gene Exploitation and Utilization in Southwest China, Wenjiang, Chengdu, Sichuan, 611130, P.R. China.
Zhao X
Triticeae Research Institute, Sichuan Agricultural University, Wenjiang, Chengdu, Sichuan, 611130, P.R. China. State Key Laboratory of Crop Gene Exploitation and Utilization in Southwest China, Wenjiang, Chengdu, Sichuan, 611130, P.R. China.
Li H
Triticeae Research Institute, Sichuan Agricultural University, Wenjiang, Chengdu, Sichuan, 611130, P.R. China.
Pu Z
College of Agronomy, Sichuan Agricultural University, Wenjiang, Chengdu, Sichuan, 611130, P.R. China.
Li W
College of Agronomy, Sichuan Agricultural University, Wenjiang, Chengdu, Sichuan, 611130, P.R. China.
Jiang Q
Triticeae Research Institute, Sichuan Agricultural University, Wenjiang, Chengdu, Sichuan, 611130, P.R. China. State Key Laboratory of Crop Gene Exploitation and Utilization in Southwest China, Wenjiang, Chengdu, Sichuan, 611130, P.R. China.
Wei Y
Triticeae Research Institute, Sichuan Agricultural University, Wenjiang, Chengdu, Sichuan, 611130, P.R. China. State Key Laboratory of Crop Gene Exploitation and Utilization in Southwest China, Wenjiang, Chengdu, Sichuan, 611130, P.R. China.
Ma J
Triticeae Research Institute, Sichuan Agricultural University, Wenjiang, Chengdu, Sichuan, 611130, P.R. China.
Kang H
Triticeae Research Institute, Sichuan Agricultural University, Wenjiang, Chengdu, Sichuan, 611130, P.R. China. State Key Laboratory of Crop Gene Exploitation and Utilization in Southwest China, Wenjiang, Chengdu, Sichuan, 611130, P.R. China.
Dai S
Triticeae Research Institute, Sichuan Agricultural University, Wenjiang, Chengdu, Sichuan, 611130, P.R. China.
Qi P
Triticeae Research Institute, Sichuan Agricultural University, Wenjiang, Chengdu, Sichuan, 611130, P.R. China.
Xu Q
Triticeae Research Institute, Sichuan Agricultural University, Wenjiang, Chengdu, Sichuan, 611130, P.R. China. State Key Laboratory of Crop Gene Exploitation and Utilization in Southwest China, Wenjiang, Chengdu, Sichuan, 611130, P.R. China.
Deng M
Triticeae Research Institute, Sichuan Agricultural University, Wenjiang, Chengdu, Sichuan, 611130, P.R. China.
Zheng Y
Triticeae Research Institute, Sichuan Agricultural University, Wenjiang, Chengdu, Sichuan, 611130, P.R. China. State Key Laboratory of Crop Gene Exploitation and Utilization in Southwest China, Wenjiang, Chengdu, Sichuan, 611130, P.R. China.
Jiang Y
Triticeae Research Institute, Sichuan Agricultural University, Wenjiang, Chengdu, Sichuan, 611130, P.R. China.
Chen G
Triticeae Research Institute, Sichuan Agricultural University, Wenjiang, Chengdu, Sichuan, 611130, P.R. China. State Key Laboratory of Crop Gene Exploitation and Utilization in Southwest China, Wenjiang, Chengdu, Sichuan, 611130, P.R. China.

MeSH

ChinaChromosomesPlant DiseasesQuantitative Trait LociTriticum

Pub Type(s)

Journal Article

Language

eng

PubMed ID

33599530

Citation

Long, Li, et al. "A Stable Quantitative Trait Locus On Chromosome 5BL Combined With Yr18 Conferring High-Level Adult Plant Resistance to Stripe Rust in Chinese Wheat Landrace Anyuehong." Phytopathology, vol. 111, no. 9, 2021, pp. 1594-1601.
Long L, Yao F, Guan F, et al. A Stable Quantitative Trait Locus on Chromosome 5BL Combined with Yr18 Conferring High-Level Adult Plant Resistance to Stripe Rust in Chinese Wheat Landrace Anyuehong. Phytopathology. 2021;111(9):1594-1601.
Long, L., Yao, F., Guan, F., Cheng, Y., Duan, L., Zhao, X., Li, H., Pu, Z., Li, W., Jiang, Q., Wei, Y., Ma, J., Kang, H., Dai, S., Qi, P., Xu, Q., Deng, M., Zheng, Y., Jiang, Y., & Chen, G. (2021). A Stable Quantitative Trait Locus on Chromosome 5BL Combined with Yr18 Conferring High-Level Adult Plant Resistance to Stripe Rust in Chinese Wheat Landrace Anyuehong. Phytopathology, 111(9), 1594-1601. https://doi.org/10.1094/PHYTO-10-20-0465-R
Long L, et al. A Stable Quantitative Trait Locus On Chromosome 5BL Combined With Yr18 Conferring High-Level Adult Plant Resistance to Stripe Rust in Chinese Wheat Landrace Anyuehong. Phytopathology. 2021;111(9):1594-1601. PubMed PMID: 33599530.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - A Stable Quantitative Trait Locus on Chromosome 5BL Combined with Yr18 Conferring High-Level Adult Plant Resistance to Stripe Rust in Chinese Wheat Landrace Anyuehong. AU - Long,Li, AU - Yao,Fangjie, AU - Guan,Fangnian, AU - Cheng,Yukun, AU - Duan,Luyao, AU - Zhao,Xuyang, AU - Li,Hao, AU - Pu,Zhien, AU - Li,Wei, AU - Jiang,Qiantao, AU - Wei,Yuming, AU - Ma,Jian, AU - Kang,Houyang, AU - Dai,Shoufen, AU - Qi,Pengfei, AU - Xu,Qiang, AU - Deng,Mei, AU - Zheng,Youliang, AU - Jiang,Yunfeng, AU - Chen,Guoyue, Y1 - 2021/10/14/ PY - 2021/2/19/pubmed PY - 2021/12/22/medline PY - 2021/2/18/entrez KW - Chinese wheat landrace KW - QTL mapping KW - disease control and pest management KW - disease resistance KW - durable resistance KW - genetics KW - kompetitive allele specific PCR (KASP) KW - yellow rust SP - 1594 EP - 1601 JF - Phytopathology JO - Phytopathology VL - 111 IS - 9 N2 - Chinese wheat landrace Anyuehong (AYH) has displayed high levels of stable adult plant resistance (APR) to stripe rust for >15 years. To identify quantitative trait loci (QTLs) for stripe rust resistance in AYH, a set of 110 recombinant inbred lines (RILs) was developed from a cross between AYH and susceptible cultivar Taichung 29. The parents and RILs were evaluated for final disease severity (FDS) in six field tests with a mixture of predominant Puccinia striiformis f. sp. tritici races at the adult plant stage and genotyped via the wheat 55K single-nucleotide polymorphism (SNP) array to construct a genetic map with 1,143 SNP markers. Three QTLs, designated as QYr.AYH-1AS, QYr.AYH-5BL, and QYr.AYH-7DS, were mapped on chromosome 1AS, 5BL, and 7DS, respectively. RILs combining three QTLs showed significantly lower FDS compared with the lines in other combinations. Of them, QYr.AYH-5BL and QYr.AYH-7DS were stably detected in all environments, explaining 13.6 to 21.4% and 17.6 to 33.6% of phenotypic variation, respectively. Compared with previous studies, QYr.AYH-5BL may be a new QTL, whereas QYr.AYH-7DS may be Yr18. Haplotype analysis revealed that QYr.AYH-5BL is probably present in 6.2% of the 323 surveyed Chinese wheat landraces. The kompetitive allele specific PCR (KASP) markers for QYr.AYH-5BL were developed by the linked SNP markers to successfully confirm the effects of the QTL in a validation population derived from a residual heterozygous line and were further assessed in 38 Chinese wheat landraces and 92 cultivars. Our results indicated that QYr.AYH-5BL with linked KASP markers has potential value for marker-assisted selection to improve stripe rust resistance in breeding programs. SN - 0031-949X UR - https://www.unboundmedicine.com/medline/citation/33599530/A_Stable_Quantitative_Trait_Locus_on_Chromosome_5BL_Combined_with_Yr18_Conferring_High_Level_Adult_Plant_Resistance_to_Stripe_Rust_in_Chinese_Wheat_Landrace_Anyuehong_ DB - PRIME DP - Unbound Medicine ER -