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Antioxidant activity and laxative effects of tannin-enriched extract of Ecklonia cava in loperamide-induced constipation of SD rats.
PLoS One. 2021; 16(2):e0246363.Plos

Abstract

To investigate the role of tannin-enriched extracts of Ecklonia cava (TEE) on the regulation of oxidative balance and laxative activity in chronic constipation, we investigated alterations after exposure to TEE, on constipation phenotypes, muscarinic cholinergic regulation, and oxidative stress responses in the transverse colons of SD rats with loperamide (Lop)-induced constipation. This extract contains high levels of total condensed tannin content (326.5 mg/g), and exhibited high inhibitory activity against 2,2-diphenyl-1-picrylhydrazyl (DPPH) radicals. TEE treatment induced significant improvements in reactive oxygen species (ROS) production, superoxide dismutase (SOD) expression and nuclear factor erythroid 2-related factor 2 (Nrf2) phosphorylation in primary smooth muscles of rat intestine cells (pRISMCs) and transverse colon of constipation model. Also, Lop+TEE treated groups showed alleviated outcomes for the following: most stool parameters, gastrointestinal transit, and intestine length were remarkably recovered; a similar recovery pattern was observed in the histopathological structure, mucin secretion, water channel expression and gastrointestinal hormones secretion in the transverse colon; expressions of muscarinic acetylcholine receptors M2/M3 (mAChR M2/M3) and their mediators on muscarinic cholinergic regulation were significantly recovered. Taken together, these results provide the first evidence that TEE stimulates oxidative stress modulation and muscarinic cholinergic regulation when exerting its laxative effects in chronic constipation models.

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Authors+Show Affiliations

Kim JE
Department of Biomaterials Science (BK21 FOUR Program), College of Natural Resources & Life Science/Life and Industry Convergence Research Institute/Laboratory Animal Resources Center, Pusan National University, Miryang, Korea.
Choi YJ
Department of Biomaterials Science (BK21 FOUR Program), College of Natural Resources & Life Science/Life and Industry Convergence Research Institute/Laboratory Animal Resources Center, Pusan National University, Miryang, Korea.
Lee SJ
Department of Biomaterials Science (BK21 FOUR Program), College of Natural Resources & Life Science/Life and Industry Convergence Research Institute/Laboratory Animal Resources Center, Pusan National University, Miryang, Korea.
Gong JE
Department of Biomaterials Science (BK21 FOUR Program), College of Natural Resources & Life Science/Life and Industry Convergence Research Institute/Laboratory Animal Resources Center, Pusan National University, Miryang, Korea.
Lee YJ
Department of Biomaterials Science (BK21 FOUR Program), College of Natural Resources & Life Science/Life and Industry Convergence Research Institute/Laboratory Animal Resources Center, Pusan National University, Miryang, Korea.
Sung JE
Department of Biomaterials Science (BK21 FOUR Program), College of Natural Resources & Life Science/Life and Industry Convergence Research Institute/Laboratory Animal Resources Center, Pusan National University, Miryang, Korea.
Jung YS
College of Pharmacy, Pusan National University, Busan, Korea.
Lee HS
Department of Food Science and Nutrition, College of Human Ecology, Pusan National University, Busan, Korea.
Hong JT
College of Pharmacy, Chungbuk National University, Chungju, Korea.
Hwang DY
Department of Biomaterials Science (BK21 FOUR Program), College of Natural Resources & Life Science/Life and Industry Convergence Research Institute/Laboratory Animal Resources Center, Pusan National University, Miryang, Korea.

MeSH

AnimalsAntioxidantsConstipationGastrointestinal TransitLaxativesLoperamideMalePhaeophytaPlant ExtractsRatsRats, Sprague-DawleyTannins

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

33626068

Citation

Kim, Ji Eun, et al. "Antioxidant Activity and Laxative Effects of Tannin-enriched Extract of Ecklonia Cava in Loperamide-induced Constipation of SD Rats." PloS One, vol. 16, no. 2, 2021, pp. e0246363.
Kim JE, Choi YJ, Lee SJ, et al. Antioxidant activity and laxative effects of tannin-enriched extract of Ecklonia cava in loperamide-induced constipation of SD rats. PLoS One. 2021;16(2):e0246363.
Kim, J. E., Choi, Y. J., Lee, S. J., Gong, J. E., Lee, Y. J., Sung, J. E., Jung, Y. S., Lee, H. S., Hong, J. T., & Hwang, D. Y. (2021). Antioxidant activity and laxative effects of tannin-enriched extract of Ecklonia cava in loperamide-induced constipation of SD rats. PloS One, 16(2), e0246363. https://doi.org/10.1371/journal.pone.0246363
Kim JE, et al. Antioxidant Activity and Laxative Effects of Tannin-enriched Extract of Ecklonia Cava in Loperamide-induced Constipation of SD Rats. PLoS One. 2021;16(2):e0246363. PubMed PMID: 33626068.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Antioxidant activity and laxative effects of tannin-enriched extract of Ecklonia cava in loperamide-induced constipation of SD rats. AU - Kim,Ji Eun, AU - Choi,Yun Ju, AU - Lee,Su Jin, AU - Gong,Jeong Eun, AU - Lee,Young Ju, AU - Sung,Ji Eun, AU - Jung,Young Suk, AU - Lee,Hee Seob, AU - Hong,Jin Tae, AU - Hwang,Dae Youn, Y1 - 2021/02/24/ PY - 2020/08/03/received PY - 2021/01/18/accepted PY - 2021/2/24/entrez PY - 2021/2/25/pubmed PY - 2021/8/4/medline SP - e0246363 EP - e0246363 JF - PloS one JO - PLoS One VL - 16 IS - 2 N2 - To investigate the role of tannin-enriched extracts of Ecklonia cava (TEE) on the regulation of oxidative balance and laxative activity in chronic constipation, we investigated alterations after exposure to TEE, on constipation phenotypes, muscarinic cholinergic regulation, and oxidative stress responses in the transverse colons of SD rats with loperamide (Lop)-induced constipation. This extract contains high levels of total condensed tannin content (326.5 mg/g), and exhibited high inhibitory activity against 2,2-diphenyl-1-picrylhydrazyl (DPPH) radicals. TEE treatment induced significant improvements in reactive oxygen species (ROS) production, superoxide dismutase (SOD) expression and nuclear factor erythroid 2-related factor 2 (Nrf2) phosphorylation in primary smooth muscles of rat intestine cells (pRISMCs) and transverse colon of constipation model. Also, Lop+TEE treated groups showed alleviated outcomes for the following: most stool parameters, gastrointestinal transit, and intestine length were remarkably recovered; a similar recovery pattern was observed in the histopathological structure, mucin secretion, water channel expression and gastrointestinal hormones secretion in the transverse colon; expressions of muscarinic acetylcholine receptors M2/M3 (mAChR M2/M3) and their mediators on muscarinic cholinergic regulation were significantly recovered. Taken together, these results provide the first evidence that TEE stimulates oxidative stress modulation and muscarinic cholinergic regulation when exerting its laxative effects in chronic constipation models. SN - 1932-6203 UR - https://www.unboundmedicine.com/medline/citation/33626068/Antioxidant_activity_and_laxative_effects_of_tannin_enriched_extract_of_Ecklonia_cava_in_loperamide_induced_constipation_of_SD_rats_ DB - PRIME DP - Unbound Medicine ER -
Grapherence [↓7 ↑37]

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