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Differential proteomic analysis of children infected with respiratory syncytial virus.
Braz J Med Biol Res. 2021; 54(4):e9850.BJ

Abstract

Respiratory syncytial virus (RSV) infection is the main cause of lower respiratory tract infection in children. However, there is no effective treatment for RSV infection. Here, we aimed to identify potential biomarkers to aid in the treatment of RSV infection. Children in the acute and convalescence phases of RSV infection were recruited and proteomic analysis was performed to identify differentially expressed proteins (DEPs). Subsequently, promising candidate proteins were determined by functional enrichment and protein-protein interaction network analysis, and underwent further validation by western blot both in clinical and mouse model samples. Among the 79 DEPs identified in RSV patient samples, 4 proteins (BPGM, TPI1, PRDX2, and CFL1) were confirmed to be significantly upregulated during RSV infection. Functional analysis showed that BPGM and TPI1 were mainly involved in glycolysis, indicating an association between RSV infection and the glycolysis metabolic pathway. Our findings provide insights into the proteomic profile during RSV infection and indicated that BPGM, TPI1, PRDX2, and CFL1 may be potential therapeutic biomarkers or targets for the treatment of RSV infection.

Links

Publisher Full Text

ncbi.nlm.nih.gov

Authors+Show Affiliations

Chronic Airways Diseases Laboratory, Department of Respiratory and Critical Care Medicine, Nanfang Hospital, Southern Medical University, Guangzhou, Guangdong, China. Guangzhou Women and Children's Medical Center, Guangzhou Medical University, Guangzhou, Guangdong, China.

Department of General Practice Medicine, the First Affiliated Hospital, Jinan University, Guangzhou, Guangdong, China.

Pediatric Research Institute, Qilu Children's Hospital of Shandong University, Jinan, Shandong, China.

Guangzhou Women and Children's Medical Center, Guangzhou Medical University, Guangzhou, Guangdong, China.

Guangzhou Women and Children's Medical Center, Guangzhou Medical University, Guangzhou, Guangdong, China.

Guangzhou Women and Children's Medical Center, Guangzhou Medical University, Guangzhou, Guangdong, China.

Guangzhou Women and Children's Medical Center, Guangzhou Medical University, Guangzhou, Guangdong, China.

Guangzhou Women and Children's Medical Center, Guangzhou Medical University, Guangzhou, Guangdong, China.

Guangzhou Women and Children's Medical Center, Guangzhou Medical University, Guangzhou, Guangdong, China.

Guangzhou Women and Children's Medical Center, Guangzhou Medical University, Guangzhou, Guangdong, China.

Guangzhou Women and Children's Medical Center, Guangzhou Medical University, Guangzhou, Guangdong, China.

Guangzhou Women and Children's Medical Center, Guangzhou Medical University, Guangzhou, Guangdong, China.

College of Computer Science, Guangdong Polytechnic Normal University, Guangzhou, Guangdong, China.

Chronic Airways Diseases Laboratory, Department of Respiratory and Critical Care Medicine, Nanfang Hospital, Southern Medical University, Guangzhou, Guangdong, China.

Guangdong Food and Drug Vocational College, Guangzhou, Guangdong, China.

Department of Cardiology, Shenzhen Children's Hospital, Shenzhen, China.

Chronic Airways Diseases Laboratory, Department of Respiratory and Critical Care Medicine, Nanfang Hospital, Southern Medical University, Guangzhou, Guangdong, China.

MeSH

BiomarkersChildHumansProteomicsRespiratory Syncytial Virus InfectionsRespiratory Syncytial Virus, Human

Pub Type(s)

Journal Article

Language

eng

PubMed ID

33656056

Citation

Yin, Gen-Quan, et al. "Differential Proteomic Analysis of Children Infected With Respiratory Syncytial Virus." Brazilian Journal of Medical and Biological Research = Revista Brasileira De Pesquisas Medicas E Biologicas, vol. 54, no. 4, 2021, pp. e9850.

Yin GQ, Zeng HX, Li ZL, et al. Differential proteomic analysis of children infected with respiratory syncytial virus. Braz J Med Biol Res. 2021;54(4):e9850.

Yin, G. Q., Zeng, H. X., Li, Z. L., Chen, C., Zhong, J. Y., Xiao, M. S., Zeng, Q., Jiang, W. H., Wu, P. Q., Zeng, J. M., Hu, X. Y., Chen, H. H., Ruo-Hu, ., Zhao, H. J., Gao, L., Liu, C., & Cai, S. X. (2021). Differential proteomic analysis of children infected with respiratory syncytial virus. Brazilian Journal of Medical and Biological Research = Revista Brasileira De Pesquisas Medicas E Biologicas, 54(4), e9850. https://doi.org/10.1590/1414-431X20209850

Yin GQ, et al. Differential Proteomic Analysis of Children Infected With Respiratory Syncytial Virus. Braz J Med Biol Res. 2021;54(4):e9850. PubMed PMID: 33656056.

* Article titles in AMA citation format should be in sentence-case

TY - JOUR T1 - Differential proteomic analysis of children infected with respiratory syncytial virus. AU - Yin,Gen-Quan, AU - Zeng,Hui-Xuan, AU - Li,Zi-Long, AU - Chen,Chen, AU - Zhong,Jia-Yong, AU - Xiao,Mi-Si, AU - Zeng,Qiang, AU - Jiang,Wen-Hui, AU - Wu,Pei-Qiong, AU - Zeng,Jie-Min, AU - Hu,Xiao-Yin, AU - Chen,Huan-Hui, AU - Ruo-Hu,, AU - Zhao,Hai-Jin, AU - Gao,Lin, AU - Liu,Cong, AU - Cai,Shao-Xi, Y1 - 2021/02/26/ PY - 2020/07/27/received PY - 2020/11/16/accepted PY - 2021/3/3/entrez PY - 2021/3/4/pubmed PY - 2021/3/5/medline SP - e9850 EP - e9850 JF - Brazilian journal of medical and biological research = Revista brasileira de pesquisas medicas e biologicas JO - Braz J Med Biol Res VL - 54 IS - 4 N2 - Respiratory syncytial virus (RSV) infection is the main cause of lower respiratory tract infection in children. However, there is no effective treatment for RSV infection. Here, we aimed to identify potential biomarkers to aid in the treatment of RSV infection. Children in the acute and convalescence phases of RSV infection were recruited and proteomic analysis was performed to identify differentially expressed proteins (DEPs). Subsequently, promising candidate proteins were determined by functional enrichment and protein-protein interaction network analysis, and underwent further validation by western blot both in clinical and mouse model samples. Among the 79 DEPs identified in RSV patient samples, 4 proteins (BPGM, TPI1, PRDX2, and CFL1) were confirmed to be significantly upregulated during RSV infection. Functional analysis showed that BPGM and TPI1 were mainly involved in glycolysis, indicating an association between RSV infection and the glycolysis metabolic pathway. Our findings provide insights into the proteomic profile during RSV infection and indicated that BPGM, TPI1, PRDX2, and CFL1 may be potential therapeutic biomarkers or targets for the treatment of RSV infection. SN - 1414-431X UR - https://www.unboundmedicine.com/medline/citation/33656056/Differential_proteomic_analysis_of_children_infected_with_respiratory_syncytial_virus. L2 - https://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2021000400609&lng=en&nrm=iso&tlng=en DB - PRIME DP - Unbound Medicine ER -