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An in vitro study of the structural properties of deep-frozen versus freeze-dried, ethylene oxide-sterilized canine anterior cruciate ligament bone-ligament-bone preparations.
Clin Orthop Relat Res 1988; (230):251-6CO

Abstract

The effect of two preparation and storage methods on the structural properties of canine anterior cruciate ligament (ACL) bone-ligament-bone preparations was determined. In Group 1, one knee joint from each dog was tested after storage at -70 degrees for eight to 12 weeks. In Group 2, one knee joint from each dog was sterilized using ethylene oxide, freeze-dried, and then stored at room temperature for four to five weeks prior to mechanical testing. In both groups, the contralateral joint was tested fresh and used as a control. The bone-ligament-bone preparations were loaded in tension until failure and the load at failure, stiffness, and maximum elongation were measured. No preparation method caused a significant decline in breaking strength. In both groups, however, there was a significant increase in elongation at failure noted in the test specimens. This was accompanied, in Group 1 (deep-frozen), by a significant decrease in stiffness in the test joints. A similar trend toward decreased stiffness was observed in the test joints from Group 2 (freeze-dried).

Authors+Show Affiliations

Department of Surgery, School of Veterinary Medicine, University of California, Davis 95616.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article

Language

eng

PubMed ID

3365899

Citation

Turner, W D., et al. "An in Vitro Study of the Structural Properties of Deep-frozen Versus Freeze-dried, Ethylene Oxide-sterilized Canine Anterior Cruciate Ligament Bone-ligament-bone Preparations." Clinical Orthopaedics and Related Research, 1988, pp. 251-6.
Turner WD, Vasseur P, Gorek JE, et al. An in vitro study of the structural properties of deep-frozen versus freeze-dried, ethylene oxide-sterilized canine anterior cruciate ligament bone-ligament-bone preparations. Clin Orthop Relat Res. 1988.
Turner, W. D., Vasseur, P., Gorek, J. E., Rodrigo, J. J., & Wedell, J. R. (1988). An in vitro study of the structural properties of deep-frozen versus freeze-dried, ethylene oxide-sterilized canine anterior cruciate ligament bone-ligament-bone preparations. Clinical Orthopaedics and Related Research, (230), pp. 251-6.
Turner WD, et al. An in Vitro Study of the Structural Properties of Deep-frozen Versus Freeze-dried, Ethylene Oxide-sterilized Canine Anterior Cruciate Ligament Bone-ligament-bone Preparations. Clin Orthop Relat Res. 1988;(230)251-6. PubMed PMID: 3365899.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - An in vitro study of the structural properties of deep-frozen versus freeze-dried, ethylene oxide-sterilized canine anterior cruciate ligament bone-ligament-bone preparations. AU - Turner,W D, AU - Vasseur,P, AU - Gorek,J E, AU - Rodrigo,J J, AU - Wedell,J R, PY - 1988/5/1/pubmed PY - 1988/5/1/medline PY - 1988/5/1/entrez SP - 251 EP - 6 JF - Clinical orthopaedics and related research JO - Clin. Orthop. Relat. Res. IS - 230 N2 - The effect of two preparation and storage methods on the structural properties of canine anterior cruciate ligament (ACL) bone-ligament-bone preparations was determined. In Group 1, one knee joint from each dog was tested after storage at -70 degrees for eight to 12 weeks. In Group 2, one knee joint from each dog was sterilized using ethylene oxide, freeze-dried, and then stored at room temperature for four to five weeks prior to mechanical testing. In both groups, the contralateral joint was tested fresh and used as a control. The bone-ligament-bone preparations were loaded in tension until failure and the load at failure, stiffness, and maximum elongation were measured. No preparation method caused a significant decline in breaking strength. In both groups, however, there was a significant increase in elongation at failure noted in the test specimens. This was accompanied, in Group 1 (deep-frozen), by a significant decrease in stiffness in the test joints. A similar trend toward decreased stiffness was observed in the test joints from Group 2 (freeze-dried). SN - 0009-921X UR - https://www.unboundmedicine.com/medline/citation/3365899/An_in_vitro_study_of_the_structural_properties_of_deep_frozen_versus_freeze_dried_ethylene_oxide_sterilized_canine_anterior_cruciate_ligament_bone_ligament_bone_preparations_ L2 - http://ovidsp.ovid.com/ovidweb.cgi?T=JS&PAGE=linkout&SEARCH=3365899.ui DB - PRIME DP - Unbound Medicine ER -