Tags

Type your tag names separated by a space and hit enter

Age-related norepinephrine induced lipolytic response of isolated rat adipocytes.
Int J Biochem 1988; 20(4):393-5IJ

Abstract

1. Groups of male Long Evans rats were sacrificed at the ages of 10, 16, 20, 24, 28, 32, 38 and 45 weeks. 2. The two epididymal fat pads from each rat in each group (4-5 rats) were excised for the preparation of adipocytes. 3. Cell suspensions were incubated in triplicate with each of seven norepinephrine concentrations ranging from 0.5694 to 569,400 nM. 4. Lipolytic responses are expressed as nmol glycerol released/microgram DNA/90 min. 5. The animals reached a peak response between the ages of 20 and 32 weeks. 6. Aging resulted in a gradual increase in the apparent affinity (Km) of the response yielding system for norepinephrine. 7. Initially an increase in the lipolytic capacity of the cells in response to norepinephrine, is observed, as reflected by the Vmax values up to an age of 20 weeks. 8. Vmax then stays relatively constant at elevated levels up to an age of 32 weeks, followed by an abrupt decrease with further aging.

Authors+Show Affiliations

Department of Biochemistry, University of Zululand, South Africa.No affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

3366297

Citation

Terblanche, S E., and W Oelofsen. "Age-related Norepinephrine Induced Lipolytic Response of Isolated Rat Adipocytes." The International Journal of Biochemistry, vol. 20, no. 4, 1988, pp. 393-5.
Terblanche SE, Oelofsen W. Age-related norepinephrine induced lipolytic response of isolated rat adipocytes. Int J Biochem. 1988;20(4):393-5.
Terblanche, S. E., & Oelofsen, W. (1988). Age-related norepinephrine induced lipolytic response of isolated rat adipocytes. The International Journal of Biochemistry, 20(4), pp. 393-5.
Terblanche SE, Oelofsen W. Age-related Norepinephrine Induced Lipolytic Response of Isolated Rat Adipocytes. Int J Biochem. 1988;20(4):393-5. PubMed PMID: 3366297.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Age-related norepinephrine induced lipolytic response of isolated rat adipocytes. AU - Terblanche,S E, AU - Oelofsen,W, PY - 1988/1/1/pubmed PY - 1988/1/1/medline PY - 1988/1/1/entrez SP - 393 EP - 5 JF - The International journal of biochemistry JO - Int. J. Biochem. VL - 20 IS - 4 N2 - 1. Groups of male Long Evans rats were sacrificed at the ages of 10, 16, 20, 24, 28, 32, 38 and 45 weeks. 2. The two epididymal fat pads from each rat in each group (4-5 rats) were excised for the preparation of adipocytes. 3. Cell suspensions were incubated in triplicate with each of seven norepinephrine concentrations ranging from 0.5694 to 569,400 nM. 4. Lipolytic responses are expressed as nmol glycerol released/microgram DNA/90 min. 5. The animals reached a peak response between the ages of 20 and 32 weeks. 6. Aging resulted in a gradual increase in the apparent affinity (Km) of the response yielding system for norepinephrine. 7. Initially an increase in the lipolytic capacity of the cells in response to norepinephrine, is observed, as reflected by the Vmax values up to an age of 20 weeks. 8. Vmax then stays relatively constant at elevated levels up to an age of 32 weeks, followed by an abrupt decrease with further aging. SN - 0020-711X UR - https://www.unboundmedicine.com/medline/citation/3366297/Age_related_norepinephrine_induced_lipolytic_response_of_isolated_rat_adipocytes_ L2 - https://medlineplus.gov/olderadulthealth.html DB - PRIME DP - Unbound Medicine ER -