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Transcriptional profiling of two contrasting genotypes uncovers molecular mechanisms underlying salt tolerance in alfalfa.
Sci Rep. 2021 03 04; 11(1):5210.SR

Abstract

Alfalfa is an important forage crop that is moderately tolerant to salinity; however, little is known about its salt-tolerance mechanisms. We studied root and leaf transcriptomes of a salt-tolerant (G03) and a salt-sensitive (G09) genotype, irrigated with waters of low and high salinities. RNA sequencing led to 1.73 billion high-quality reads that were assembled into 418,480 unigenes; 35% of which were assigned to 57 Gene Ontology annotations. The unigenes were assigned to pathway databases for understanding high-level functions. The comparison of two genotypes suggested that the low salt tolerance index for transpiration rate and stomatal conductance of G03 compared to G09 may be due to its reduced salt uptake under salinity. The differences in shoot biomass between the salt-tolerant and salt-sensitive lines were explained by their differential expressions of genes regulating shoot number. Differentially expressed genes involved in hormone-, calcium-, and redox-signaling, showed treatment- and genotype-specific differences and led to the identification of various candidate genes involved in salinity stress, which can be investigated further to improve salinity tolerance in alfalfa. Validation of RNA-seq results using qRT-PCR displayed a high level of consistency between the two experiments. This study provides valuable insight into the molecular mechanisms regulating salt tolerance in alfalfa.

Authors+Show Affiliations

Department of Plants, Soils and Climate/Center for Integrated BioSystems, College of Agriculture and Applied Sciences, Utah State University, Logan, UT, 84322, USA. rkaundal@usu.edu.Department of Plants, Soils and Climate/Center for Integrated BioSystems, College of Agriculture and Applied Sciences, Utah State University, Logan, UT, 84322, USA.US Salinity Lab (USDA-ARS), 450 W Big Springs Road, Riverside, CA, 92507, USA. College of Natural and Agricultural Sciences, University of California Riverside, Riverside, CA, 92507, USA.US Salinity Lab (USDA-ARS), 450 W Big Springs Road, Riverside, CA, 92507, USA.US Salinity Lab (USDA-ARS), 450 W Big Springs Road, Riverside, CA, 92507, USA.US Salinity Lab (USDA-ARS), 450 W Big Springs Road, Riverside, CA, 92507, USA.US Salinity Lab (USDA-ARS), 450 W Big Springs Road, Riverside, CA, 92507, USA. devinder.sandhu@ars.usda.gov.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.

Language

eng

PubMed ID

33664362

Citation

Kaundal, Rakesh, et al. "Transcriptional Profiling of Two Contrasting Genotypes Uncovers Molecular Mechanisms Underlying Salt Tolerance in Alfalfa." Scientific Reports, vol. 11, no. 1, 2021, p. 5210.
Kaundal R, Duhan N, Acharya BR, et al. Transcriptional profiling of two contrasting genotypes uncovers molecular mechanisms underlying salt tolerance in alfalfa. Sci Rep. 2021;11(1):5210.
Kaundal, R., Duhan, N., Acharya, B. R., Pudussery, M. V., Ferreira, J. F. S., Suarez, D. L., & Sandhu, D. (2021). Transcriptional profiling of two contrasting genotypes uncovers molecular mechanisms underlying salt tolerance in alfalfa. Scientific Reports, 11(1), 5210. https://doi.org/10.1038/s41598-021-84461-w
Kaundal R, et al. Transcriptional Profiling of Two Contrasting Genotypes Uncovers Molecular Mechanisms Underlying Salt Tolerance in Alfalfa. Sci Rep. 2021 03 4;11(1):5210. PubMed PMID: 33664362.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Transcriptional profiling of two contrasting genotypes uncovers molecular mechanisms underlying salt tolerance in alfalfa. AU - Kaundal,Rakesh, AU - Duhan,Naveen, AU - Acharya,Biswa R, AU - Pudussery,Manju V, AU - Ferreira,Jorge F S, AU - Suarez,Donald L, AU - Sandhu,Devinder, Y1 - 2021/03/04/ PY - 2020/01/10/received PY - 2021/02/12/accepted PY - 2021/3/5/entrez PY - 2021/3/6/pubmed PY - 2021/12/18/medline SP - 5210 EP - 5210 JF - Scientific reports JO - Sci Rep VL - 11 IS - 1 N2 - Alfalfa is an important forage crop that is moderately tolerant to salinity; however, little is known about its salt-tolerance mechanisms. We studied root and leaf transcriptomes of a salt-tolerant (G03) and a salt-sensitive (G09) genotype, irrigated with waters of low and high salinities. RNA sequencing led to 1.73 billion high-quality reads that were assembled into 418,480 unigenes; 35% of which were assigned to 57 Gene Ontology annotations. The unigenes were assigned to pathway databases for understanding high-level functions. The comparison of two genotypes suggested that the low salt tolerance index for transpiration rate and stomatal conductance of G03 compared to G09 may be due to its reduced salt uptake under salinity. The differences in shoot biomass between the salt-tolerant and salt-sensitive lines were explained by their differential expressions of genes regulating shoot number. Differentially expressed genes involved in hormone-, calcium-, and redox-signaling, showed treatment- and genotype-specific differences and led to the identification of various candidate genes involved in salinity stress, which can be investigated further to improve salinity tolerance in alfalfa. Validation of RNA-seq results using qRT-PCR displayed a high level of consistency between the two experiments. This study provides valuable insight into the molecular mechanisms regulating salt tolerance in alfalfa. SN - 2045-2322 UR - https://www.unboundmedicine.com/medline/citation/33664362/Transcriptional_profiling_of_two_contrasting_genotypes_uncovers_molecular_mechanisms_underlying_salt_tolerance_in_alfalfa_ DB - PRIME DP - Unbound Medicine ER -