Tags

Type your tag names separated by a space and hit enter

Metagenomic profiling of rhizosphere microbial community structure and diversity associated with maize plant as affected by cropping systems.
Int Microbiol. 2021 Aug; 24(3):325-335.IM

Abstract

Soil microbial diversity is believed to be vital in maintaining soil quality and health. Limited knowledge exists on the impact of cropping systems (mono-cropping and crop rotation) on the diversity of the whole soil microbiome. In this study, we investigated the effects of two cropping systems, namely crop rotation and mono-cropping, on the community structure and diversity of rhizosphere microbiome in the rhizosphere and bulk soil associated with maize plant using shotgun metagenomics. Whole DNA was extracted from bulk, and rhizosphere soils associated with maize plant from the mono-cropping (LT and LTc) and crop rotation (VD and VDc) sites, respectively, and sequenced employing shotgun metagenomics. The results obtained via the Subsystem database showed 23 bacteria, 2 fungi, and 3 archaea most abundant phyla. The major bacterial phyla are Firmicutes, Bacteroidetes, Actinobacteria, Proteobacteria, Gemmatimonadetes, Acidobacteria, Cyanobacteria, Spirochaetes, Aquificae, Verrucomicrobia, Chloroflexi, Planctomycetes, and Chlorobi. The major fungi phyla observed were Ascomycota and Basidiomycota, while the dominant archaea phyla are Euryarchaeota, Thaumarchaeota, and Crenarchaeota. Our diversity assessment showed that the rhizosphere microbial community was more abundant in the samples from the rotational crop site following VD>VDc>LT>LTc. Alpha diversity showed that there was no significant difference (P>0.05) in the soil microbial communities (P>0.05), while better diversity indicated that a significant difference (P = 0.01) occurred. Taken together, crop rotational practice was found to positively influence the rhizosphere microbial community associated with the maize plant.

Links

Publisher Full Text (DOI)

Authors+Show Affiliations

Fadiji AE
Food Security and Safety Niche, Faculty of Natural and Agricultural Sciences, North-West University, Private Mail Bag X2046, Mmabatho, South Africa.
Kanu JO
Food Security and Safety Niche, Faculty of Natural and Agricultural Sciences, North-West University, Private Mail Bag X2046, Mmabatho, South Africa.
Babalola OO
Food Security and Safety Niche, Faculty of Natural and Agricultural Sciences, North-West University, Private Mail Bag X2046, Mmabatho, South Africa. olubukola.babalola@nwu.ac.za.

MeSH

AgricultureArchaeaBacteriaBiodiversityFungiMetagenomicsMicrobiotaPlant RootsRNA, Ribosomal, 16SRhizosphereSoil MicrobiologyZea mays

Pub Type(s)

Journal Article

Language

eng

PubMed ID

33666787

Citation

Fadiji, Ayomide Emmanuel, et al. "Metagenomic Profiling of Rhizosphere Microbial Community Structure and Diversity Associated With Maize Plant as Affected By Cropping Systems." International Microbiology : the Official Journal of the Spanish Society for Microbiology, vol. 24, no. 3, 2021, pp. 325-335.
Fadiji AE, Kanu JO, Babalola OO. Metagenomic profiling of rhizosphere microbial community structure and diversity associated with maize plant as affected by cropping systems. Int Microbiol. 2021;24(3):325-335.
Fadiji, A. E., Kanu, J. O., & Babalola, O. O. (2021). Metagenomic profiling of rhizosphere microbial community structure and diversity associated with maize plant as affected by cropping systems. International Microbiology : the Official Journal of the Spanish Society for Microbiology, 24(3), 325-335. https://doi.org/10.1007/s10123-021-00169-x
Fadiji AE, Kanu JO, Babalola OO. Metagenomic Profiling of Rhizosphere Microbial Community Structure and Diversity Associated With Maize Plant as Affected By Cropping Systems. Int Microbiol. 2021;24(3):325-335. PubMed PMID: 33666787.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Metagenomic profiling of rhizosphere microbial community structure and diversity associated with maize plant as affected by cropping systems. AU - Fadiji,Ayomide Emmanuel, AU - Kanu,Jerry Onyemaechi, AU - Babalola,Olubukola Oluranti, Y1 - 2021/03/05/ PY - 2021/01/13/received PY - 2021/02/19/accepted PY - 2021/02/15/revised PY - 2021/3/6/pubmed PY - 2021/11/20/medline PY - 2021/3/5/entrez KW - Crop rotation KW - Rhizosphere soil KW - Shotgun metagenomics KW - Sustainable agriculture SP - 325 EP - 335 JF - International microbiology : the official journal of the Spanish Society for Microbiology JO - Int Microbiol VL - 24 IS - 3 N2 - Soil microbial diversity is believed to be vital in maintaining soil quality and health. Limited knowledge exists on the impact of cropping systems (mono-cropping and crop rotation) on the diversity of the whole soil microbiome. In this study, we investigated the effects of two cropping systems, namely crop rotation and mono-cropping, on the community structure and diversity of rhizosphere microbiome in the rhizosphere and bulk soil associated with maize plant using shotgun metagenomics. Whole DNA was extracted from bulk, and rhizosphere soils associated with maize plant from the mono-cropping (LT and LTc) and crop rotation (VD and VDc) sites, respectively, and sequenced employing shotgun metagenomics. The results obtained via the Subsystem database showed 23 bacteria, 2 fungi, and 3 archaea most abundant phyla. The major bacterial phyla are Firmicutes, Bacteroidetes, Actinobacteria, Proteobacteria, Gemmatimonadetes, Acidobacteria, Cyanobacteria, Spirochaetes, Aquificae, Verrucomicrobia, Chloroflexi, Planctomycetes, and Chlorobi. The major fungi phyla observed were Ascomycota and Basidiomycota, while the dominant archaea phyla are Euryarchaeota, Thaumarchaeota, and Crenarchaeota. Our diversity assessment showed that the rhizosphere microbial community was more abundant in the samples from the rotational crop site following VD>VDc>LT>LTc. Alpha diversity showed that there was no significant difference (P>0.05) in the soil microbial communities (P>0.05), while better diversity indicated that a significant difference (P = 0.01) occurred. Taken together, crop rotational practice was found to positively influence the rhizosphere microbial community associated with the maize plant. SN - 1618-1905 UR - https://www.unboundmedicine.com/medline/citation/33666787/Metagenomic_profiling_of_rhizosphere_microbial_community_structure_and_diversity_associated_with_maize_plant_as_affected_by_cropping_systems_ DB - PRIME DP - Unbound Medicine ER -