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Co-expression of the SARS-CoV-2 entry receptors ACE2 and TMPRSS2 in healthy human conjunctiva.
Exp Eye Res. 2021 04; 205:108527.EE

Abstract

The purpose of this study was to evaluate the expression of the SARS-CoV-2 receptors ACE2 and TMPRSS2 in an immortalized human conjunctival epithelial cell line and in healthy human conjunctiva excised during ocular surgery, using Western blot, confocal microscopy and immunohistochemistry. The Western blot showed that ACE2 and TMPRSS2 proteins were expressed in human immortalized conjunctival cells, and this was confirmed by confocal microscopy images, that demonstrated a marked cellular expression of the viral receptors and their co-localization on the cell membranes. Healthy conjunctival samples from 11 adult patients were excised during retinal detachment surgery. We found the expression of ACE2 and TMPRSS2 in all the conjunctival surgical specimens analyzed and their co-localization in the superficial conjunctival epithelium. The ACE2 Western blot levels and immunofluorescence staining for ACE2 were variable among specimens. These results suggest the susceptibility of the conjunctival epithelium to SARS-CoV-2 infection, even though with a possible interindividual variability.

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Authors+Show Affiliations

Mencucci R
Eye Clinic, Department of Neurosciences, Psychology, Pharmacology and Child Health (NEUROFARBA), University of Florence, Largo Brambilla 3, 50134, Florence, Italy. Electronic address: rita.mencucci@unifi.it.
Favuzza E
Eye Clinic, Department of Neurosciences, Psychology, Pharmacology and Child Health (NEUROFARBA), University of Florence, Largo Brambilla 3, 50134, Florence, Italy. Electronic address: elefavuzza@gmail.com.
Becatti M
Department of Experimental and Clinical Biomedical Sciences "Mario Serio", University of Florence, Viale Morgagni 50, 50134, Florence, Italy. Electronic address: matteo.becatti@unifi.it.
Tani A
Department of Experimental and Clinical Medicine, Section of Anatomy and Histology, University of Florence, Largo Brambilla 3, 50134, Florence, Italy. Electronic address: alessia.tani@unifi.it.
Mazzantini C
Department of Health Sciences, Section of Clinical Pharmacology and Oncology, University of Florence, Viale Pieraccini 6, 50139, Florence, Italy. Electronic address: costanza.mazzantini@unifi.it.
Vignapiano R
Eye Clinic, Department of Neurosciences, Psychology, Pharmacology and Child Health (NEUROFARBA), University of Florence, Largo Brambilla 3, 50134, Florence, Italy. Electronic address: roberto.vignapiano@gmail.com.
Fiorillo C
Department of Experimental and Clinical Biomedical Sciences "Mario Serio", University of Florence, Viale Morgagni 50, 50134, Florence, Italy. Electronic address: claudia.fiorillo@unifi.it.
Pellegrini-Giampietro D
Department of Health Sciences, Section of Clinical Pharmacology and Oncology, University of Florence, Viale Pieraccini 6, 50139, Florence, Italy. Electronic address: domenico.pellegrini@unifi.it.
Manetti M
Department of Experimental and Clinical Medicine, Section of Anatomy and Histology, University of Florence, Largo Brambilla 3, 50134, Florence, Italy. Electronic address: mirko.manetti@unifi.it.
Marini M
Department of Experimental and Clinical Medicine, Section of Anatomy and Histology, University of Florence, Largo Brambilla 3, 50134, Florence, Italy. Electronic address: mirca.marini@unifi.it.
Landucci E
Department of Health Sciences, Section of Clinical Pharmacology and Oncology, University of Florence, Viale Pieraccini 6, 50139, Florence, Italy. Electronic address: elisa.landucci@unifi.it.

MeSH

COVID-19ConjunctivaEpithelial CellsGene Expression RegulationHumansImmunohistochemistryPeptidyl-Dipeptidase ARNASARS-CoV-2Serine Endopeptidases

Pub Type(s)

Journal Article

Language

eng

PubMed ID

33667466

Citation

Mencucci, Rita, et al. "Co-expression of the SARS-CoV-2 Entry Receptors ACE2 and TMPRSS2 in Healthy Human Conjunctiva." Experimental Eye Research, vol. 205, 2021, p. 108527.
Mencucci R, Favuzza E, Becatti M, et al. Co-expression of the SARS-CoV-2 entry receptors ACE2 and TMPRSS2 in healthy human conjunctiva. Exp Eye Res. 2021;205:108527.
Mencucci, R., Favuzza, E., Becatti, M., Tani, A., Mazzantini, C., Vignapiano, R., Fiorillo, C., Pellegrini-Giampietro, D., Manetti, M., Marini, M., & Landucci, E. (2021). Co-expression of the SARS-CoV-2 entry receptors ACE2 and TMPRSS2 in healthy human conjunctiva. Experimental Eye Research, 205, 108527. https://doi.org/10.1016/j.exer.2021.108527
Mencucci R, et al. Co-expression of the SARS-CoV-2 Entry Receptors ACE2 and TMPRSS2 in Healthy Human Conjunctiva. Exp Eye Res. 2021;205:108527. PubMed PMID: 33667466.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Co-expression of the SARS-CoV-2 entry receptors ACE2 and TMPRSS2 in healthy human conjunctiva. AU - Mencucci,Rita, AU - Favuzza,Eleonora, AU - Becatti,Matteo, AU - Tani,Alessia, AU - Mazzantini,Costanza, AU - Vignapiano,Roberto, AU - Fiorillo,Claudia, AU - Pellegrini-Giampietro,DomenicoE, AU - Manetti,Mirko, AU - Marini,Mirca, AU - Landucci,Elisa, Y1 - 2021/03/02/ PY - 2020/12/15/received PY - 2021/02/10/revised PY - 2021/02/22/accepted PY - 2021/3/6/pubmed PY - 2021/4/21/medline PY - 2021/3/5/entrez KW - ACE2 KW - COVID-19 KW - Conjunctival epithelial cells KW - Human conjunctiva KW - Immunohistochemistry KW - SARS-CoV-2 KW - TMPRSS2 KW - Western blot SP - 108527 EP - 108527 JF - Experimental eye research JO - Exp Eye Res VL - 205 N2 - The purpose of this study was to evaluate the expression of the SARS-CoV-2 receptors ACE2 and TMPRSS2 in an immortalized human conjunctival epithelial cell line and in healthy human conjunctiva excised during ocular surgery, using Western blot, confocal microscopy and immunohistochemistry. The Western blot showed that ACE2 and TMPRSS2 proteins were expressed in human immortalized conjunctival cells, and this was confirmed by confocal microscopy images, that demonstrated a marked cellular expression of the viral receptors and their co-localization on the cell membranes. Healthy conjunctival samples from 11 adult patients were excised during retinal detachment surgery. We found the expression of ACE2 and TMPRSS2 in all the conjunctival surgical specimens analyzed and their co-localization in the superficial conjunctival epithelium. The ACE2 Western blot levels and immunofluorescence staining for ACE2 were variable among specimens. These results suggest the susceptibility of the conjunctival epithelium to SARS-CoV-2 infection, even though with a possible interindividual variability. SN - 1096-0007 UR - https://www.unboundmedicine.com/medline/citation/33667466/Co_expression_of_the_SARS_CoV_2_entry_receptors_ACE2_and_TMPRSS2_in_healthy_human_conjunctiva_ DB - PRIME DP - Unbound Medicine ER -