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Clinical performance of a point-of-care Coccidioides antibody test in dogs.
J Vet Intern Med. 2021 Mar; 35(2):965-969.JV

Abstract

BACKGROUND

Point-of-care (POC) Coccidioides antibody assays may provide veterinarians with rapid and accurate diagnostic information.

OBJECTIVES

To determine the agreement of a POC lateral flow assay (LFA), sona Coccidioides (IMMY, Norman, Oklahoma) with the current diagnostic standard, the immunodiffusion assay (agar gel immunodiffusion [AGID]; Coccidioidomycosis Serology Laboratory, University of California, Davis, California).

ANIMALS

Forty-eight sera specimens from 48 dogs.

METHODS

Sera specimens were collected from client-owned dogs that had a clinical suspicion for coccidioidomycosis. Animals were classified as Coccidioides antibody-positive (n = 36) based on a positive AGID or Coccidioides antibody-negative (n = 12) based on a negative AGID. The performance of the LFA assay was determined by comparing results to AGID results.

RESULTS

The LFA assay demonstrated agreement in 32 of 36 Coccidioides antibody-positive specimens and 12 of 12 Coccidioides antibody-negative specimens, resulting in a positive percentage agreement of 88.9% (95% confidence interval [CI], 74.7-95.6%) and negative percentage agreement of 100% (95% CI, 75.8-100%) as compared to AGID. A receiver operator characteristic curve was constructed, and the area under the curve was 0.944 (CI, 0.880-1.000).

CONCLUSION AND CLINICAL IMPORTANCE

This LFA is a rapid alternative to the traditional AGID. The LFA provides excellent predictive value for positive results. Positive agreement was lower in dogs with low AGID titers; therefore, confirmatory testing is recommended if a high index of suspicion exists.

Authors+Show Affiliations

Department of Medicine and Epidemiology, University of California-Davis, Davis, California, USA.Department of Medical Microbiology and Immunology, University of California-Davis, Davis, California, USA.Department of Internal Medicine, Division of Infectious Diseases, University of California Davis Medical Center, Sacramento, California, USA.Department of Medicine and Epidemiology, University of California-Davis, Davis, California, USA.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

33675146

Citation

Reagan, Krystle L., et al. "Clinical Performance of a Point-of-care Coccidioides Antibody Test in Dogs." Journal of Veterinary Internal Medicine, vol. 35, no. 2, 2021, pp. 965-969.
Reagan KL, McHardy I, Thompson GR, et al. Clinical performance of a point-of-care Coccidioides antibody test in dogs. J Vet Intern Med. 2021;35(2):965-969.
Reagan, K. L., McHardy, I., Thompson, G. R., & Sykes, J. E. (2021). Clinical performance of a point-of-care Coccidioides antibody test in dogs. Journal of Veterinary Internal Medicine, 35(2), 965-969. https://doi.org/10.1111/jvim.16087
Reagan KL, et al. Clinical Performance of a Point-of-care Coccidioides Antibody Test in Dogs. J Vet Intern Med. 2021;35(2):965-969. PubMed PMID: 33675146.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Clinical performance of a point-of-care Coccidioides antibody test in dogs. AU - Reagan,Krystle L, AU - McHardy,Ian, AU - Thompson,George R,3rd AU - Sykes,Jane E, Y1 - 2021/03/06/ PY - 2021/02/09/revised PY - 2020/11/30/received PY - 2021/02/19/accepted PY - 2021/3/7/pubmed PY - 2021/3/7/medline PY - 2021/3/6/entrez KW - diagnostic test KW - fungal KW - lateral flow KW - veterinary SP - 965 EP - 969 JF - Journal of veterinary internal medicine JO - J Vet Intern Med VL - 35 IS - 2 N2 - BACKGROUND: Point-of-care (POC) Coccidioides antibody assays may provide veterinarians with rapid and accurate diagnostic information. OBJECTIVES: To determine the agreement of a POC lateral flow assay (LFA), sona Coccidioides (IMMY, Norman, Oklahoma) with the current diagnostic standard, the immunodiffusion assay (agar gel immunodiffusion [AGID]; Coccidioidomycosis Serology Laboratory, University of California, Davis, California). ANIMALS: Forty-eight sera specimens from 48 dogs. METHODS: Sera specimens were collected from client-owned dogs that had a clinical suspicion for coccidioidomycosis. Animals were classified as Coccidioides antibody-positive (n = 36) based on a positive AGID or Coccidioides antibody-negative (n = 12) based on a negative AGID. The performance of the LFA assay was determined by comparing results to AGID results. RESULTS: The LFA assay demonstrated agreement in 32 of 36 Coccidioides antibody-positive specimens and 12 of 12 Coccidioides antibody-negative specimens, resulting in a positive percentage agreement of 88.9% (95% confidence interval [CI], 74.7-95.6%) and negative percentage agreement of 100% (95% CI, 75.8-100%) as compared to AGID. A receiver operator characteristic curve was constructed, and the area under the curve was 0.944 (CI, 0.880-1.000). CONCLUSION AND CLINICAL IMPORTANCE: This LFA is a rapid alternative to the traditional AGID. The LFA provides excellent predictive value for positive results. Positive agreement was lower in dogs with low AGID titers; therefore, confirmatory testing is recommended if a high index of suspicion exists. SN - 1939-1676 UR - https://www.unboundmedicine.com/medline/citation/33675146/Clinical_performance_of_a_point_of_care_Coccidioides_antibody_test_in_dogs_ L2 - https://doi.org/10.1111/jvim.16087 DB - PRIME DP - Unbound Medicine ER -