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Chromosomal composition analysis and molecular marker development for the novel Ug99-resistant wheat-Thinopyrum ponticum translocation line WTT34.
Theor Appl Genet. 2021 May; 134(5):1587-1599.TA

Abstract

KEY MESSAGE

A novel Ug99-resistant wheat-Thinopyrum ponticum translocation line was produced, its chromosomal composition was analyzed and specific markers were developed. Stem rust caused by Puccinia graminis f. sp. tritici Eriks. & E. Henn (Pgt) has seriously threatened global wheat production since Ug99 race TTKSK was first detected in Uganda in 1998. Thinopyrum ponticum is near immune to Ug99 races and may be useful for enhancing wheat disease resistance. Therefore, developing new wheat-Th. ponticum translocation lines that are resistant to Ug99 is crucial. In this study, a novel wheat-Th. ponticum translocation line, WTT34, was produced. Seedling and field evaluation revealed that WTT34 is resistant to Ug99 race PTKST. The resistance was derived from the alien parent Th. ponticum. Screening WTT34 with markers linked to Sr24, Sr25, Sr26, Sr43, and SrB resulted in the amplification of different DNA fragments from Th. ponticum, implying WTT34 carries at least one novel stem rust resistance gene. Genomic in situ hybridization (GISH), multicolor fluorescence in situ hybridization (mc-FISH), and multi-color GISH (mc-GISH) analyses indicated that WTT34 carries a T5DS·5DL-Th translocation, which was consistent with wheat660K single-nucleotide polymorphism (SNP) array results. The SNP array also uncovered a deletion event in the terminal region of chromosome 1D. Additionally, the homeology between alien segments and the wheat chromosomes 2A and 5D was confirmed. Furthermore, 51 PCR-based markers derived from the alien segments of WTT34 were developed based on specific-locus amplified fragment sequencing (SLAF-seq). These markers may enable wheat breeders to rapidly trace Th. ponticum chromosomal segments carrying Ug99 resistance gene(s).

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Publisher Full Text (DOI)

Authors+Show Affiliations

Yang G
State Key Laboratory of Plant Cell and Chromosome Engineering, Institute of Genetics and Developmental Biology, The Innovative Academy of Seed Design, Chinese Academy of Sciences, Beijing, 100101, China. University of Chinese Academy of Sciences, Beijing, 100049, China.
Boshoff WHP
Department of Plant Sciences, University of the Free State, Bloemfontein, 9300, South Africa.
Li H
State Key Laboratory of Plant Cell and Chromosome Engineering, Institute of Genetics and Developmental Biology, The Innovative Academy of Seed Design, Chinese Academy of Sciences, Beijing, 100101, China.
Pretorius ZA
Department of Plant Sciences, University of the Free State, Bloemfontein, 9300, South Africa.
Luo Q
State Key Laboratory of Plant Cell and Chromosome Engineering, Institute of Genetics and Developmental Biology, The Innovative Academy of Seed Design, Chinese Academy of Sciences, Beijing, 100101, China.
Li B
State Key Laboratory of Plant Cell and Chromosome Engineering, Institute of Genetics and Developmental Biology, The Innovative Academy of Seed Design, Chinese Academy of Sciences, Beijing, 100101, China.
Li Z
State Key Laboratory of Plant Cell and Chromosome Engineering, Institute of Genetics and Developmental Biology, The Innovative Academy of Seed Design, Chinese Academy of Sciences, Beijing, 100101, China.
Zheng Q
State Key Laboratory of Plant Cell and Chromosome Engineering, Institute of Genetics and Developmental Biology, The Innovative Academy of Seed Design, Chinese Academy of Sciences, Beijing, 100101, China. qzheng@genetics.ac.cn.

MeSH

BasidiomycotaChromosome MappingChromosomes, PlantDisease ResistanceGenetic MarkersGenome, PlantPlant BreedingPlant DiseasesPolymorphism, Single NucleotideQuantitative Trait LociTranslocation, GeneticTriticum

Pub Type(s)

Journal Article

Language

eng

PubMed ID

33677639

Citation

Yang, Guotang, et al. "Chromosomal Composition Analysis and Molecular Marker Development for the Novel Ug99-resistant wheat-Thinopyrum Ponticum Translocation Line WTT34." TAG. Theoretical and Applied Genetics. Theoretische Und Angewandte Genetik, vol. 134, no. 5, 2021, pp. 1587-1599.
Yang G, Boshoff WHP, Li H, et al. Chromosomal composition analysis and molecular marker development for the novel Ug99-resistant wheat-Thinopyrum ponticum translocation line WTT34. Theor Appl Genet. 2021;134(5):1587-1599.
Yang, G., Boshoff, W. H. P., Li, H., Pretorius, Z. A., Luo, Q., Li, B., Li, Z., & Zheng, Q. (2021). Chromosomal composition analysis and molecular marker development for the novel Ug99-resistant wheat-Thinopyrum ponticum translocation line WTT34. TAG. Theoretical and Applied Genetics. Theoretische Und Angewandte Genetik, 134(5), 1587-1599. https://doi.org/10.1007/s00122-021-03796-0
Yang G, et al. Chromosomal Composition Analysis and Molecular Marker Development for the Novel Ug99-resistant wheat-Thinopyrum Ponticum Translocation Line WTT34. Theor Appl Genet. 2021;134(5):1587-1599. PubMed PMID: 33677639.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Chromosomal composition analysis and molecular marker development for the novel Ug99-resistant wheat-Thinopyrum ponticum translocation line WTT34. AU - Yang,Guotang, AU - Boshoff,Willem H P, AU - Li,Hongwei, AU - Pretorius,Zacharias A, AU - Luo,Qiaoling, AU - Li,Bin, AU - Li,Zhensheng, AU - Zheng,Qi, Y1 - 2021/03/06/ PY - 2020/07/12/received PY - 2021/02/16/accepted PY - 2021/3/8/pubmed PY - 2021/8/25/medline PY - 2021/3/7/entrez SP - 1587 EP - 1599 JF - TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik JO - Theor Appl Genet VL - 134 IS - 5 N2 - KEY MESSAGE: A novel Ug99-resistant wheat-Thinopyrum ponticum translocation line was produced, its chromosomal composition was analyzed and specific markers were developed. Stem rust caused by Puccinia graminis f. sp. tritici Eriks. & E. Henn (Pgt) has seriously threatened global wheat production since Ug99 race TTKSK was first detected in Uganda in 1998. Thinopyrum ponticum is near immune to Ug99 races and may be useful for enhancing wheat disease resistance. Therefore, developing new wheat-Th. ponticum translocation lines that are resistant to Ug99 is crucial. In this study, a novel wheat-Th. ponticum translocation line, WTT34, was produced. Seedling and field evaluation revealed that WTT34 is resistant to Ug99 race PTKST. The resistance was derived from the alien parent Th. ponticum. Screening WTT34 with markers linked to Sr24, Sr25, Sr26, Sr43, and SrB resulted in the amplification of different DNA fragments from Th. ponticum, implying WTT34 carries at least one novel stem rust resistance gene. Genomic in situ hybridization (GISH), multicolor fluorescence in situ hybridization (mc-FISH), and multi-color GISH (mc-GISH) analyses indicated that WTT34 carries a T5DS·5DL-Th translocation, which was consistent with wheat660K single-nucleotide polymorphism (SNP) array results. The SNP array also uncovered a deletion event in the terminal region of chromosome 1D. Additionally, the homeology between alien segments and the wheat chromosomes 2A and 5D was confirmed. Furthermore, 51 PCR-based markers derived from the alien segments of WTT34 were developed based on specific-locus amplified fragment sequencing (SLAF-seq). These markers may enable wheat breeders to rapidly trace Th. ponticum chromosomal segments carrying Ug99 resistance gene(s). SN - 1432-2242 UR - https://www.unboundmedicine.com/medline/citation/33677639/Chromosomal_composition_analysis_and_molecular_marker_development_for_the_novel_Ug99_resistant_wheat_Thinopyrum_ponticum_translocation_line_WTT34_ DB - PRIME DP - Unbound Medicine ER -