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Identification and expression analysis of the WRKY gene family in Isatis indigotica.
Gene. 2021 May 30; 783:145561.GENE

Abstract

The WRKY proteins, which represent one of the largest families of transcriptional regulators in plants, play pivotal roles in regulating multiple processes of growth and development, particularly in diverse stress responses. Isatis indigotica is widely used in Traditional Chinese Medicine and is famous for its use as a dye for the color indigo. However, reports of the WRKY gene family in I. indigotica are limited. In this study, 64 IiWRKY genes encoding proteins with the complete WRKY domain were identified from genome of I. indigotica. Based on their structure and phylogenetic relationships of this gene family in I. indigotica, the IiWRKY genes were classified into three groups: Group I (n = 13), Group II (n = 35) and Group III (n = 16). Sequence alignment revealed that IiWRKY proteins harbored two variants, WRKYRQK and WRKYGKK, of the highly conserved WRKYGQK motif. The number of exons in IiWRKY genes varied from two to 14, with most of IiWRKY genes containing three exons. Investigation of gene duplication demonstrated that 10 and 14 IiWRKY genes were incorporated in tandem and segmental duplication events, respectively. Finally, the expression profiles derived from transcriptome data and quantitative real-time PCR analysis showed distinct expression patterns of these IiWRKY gene in five different organs or in response to four abiotic stresses. Taken together, our results will contribute to functional analysis of IiWRKY genes, and also provide a basis for further clarification of the molecular mechanism of stress responses in this important herb.

Authors+Show Affiliations

College of Horticulture, Nanjing Agricultural University, Nanjing 210095, China. Electronic address: 2017104126@njau.edu.cn.College of Horticulture, Nanjing Agricultural University, Nanjing 210095, China. Electronic address: 2017104125@njau.edu.cn.College of Horticulture, Nanjing Agricultural University, Nanjing 210095, China. Electronic address: xqtang@njau.edu.cn.College of Horticulture, Nanjing Agricultural University, Nanjing 210095, China. Electronic address: liqiongsun@njau.edu.cn.College of Horticulture, Nanjing Agricultural University, Nanjing 210095, China. Electronic address: 2018104128@njau.edu.cn.College of Horticulture, Nanjing Agricultural University, Nanjing 210095, China. Electronic address: wangkc@njau.edu.cn.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

33705810

Citation

Qu, Renjun, et al. "Identification and Expression Analysis of the WRKY Gene Family in Isatis Indigotica." Gene, vol. 783, 2021, p. 145561.
Qu R, Cao Y, Tang X, et al. Identification and expression analysis of the WRKY gene family in Isatis indigotica. Gene. 2021;783:145561.
Qu, R., Cao, Y., Tang, X., Sun, L., Wei, L., & Wang, K. (2021). Identification and expression analysis of the WRKY gene family in Isatis indigotica. Gene, 783, 145561. https://doi.org/10.1016/j.gene.2021.145561
Qu R, et al. Identification and Expression Analysis of the WRKY Gene Family in Isatis Indigotica. Gene. 2021 May 30;783:145561. PubMed PMID: 33705810.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Identification and expression analysis of the WRKY gene family in Isatis indigotica. AU - Qu,Renjun, AU - Cao,Yiwen, AU - Tang,Xiaoqing, AU - Sun,Liqiong, AU - Wei,Lian, AU - Wang,Kangcai, Y1 - 2021/03/09/ PY - 2020/03/17/received PY - 2021/01/14/revised PY - 2021/03/01/accepted PY - 2021/3/12/pubmed PY - 2021/4/23/medline PY - 2021/3/11/entrez KW - Abiotic stress KW - Genome KW - Isatis indigotica KW - WRKY transcription factor family SP - 145561 EP - 145561 JF - Gene JO - Gene VL - 783 N2 - The WRKY proteins, which represent one of the largest families of transcriptional regulators in plants, play pivotal roles in regulating multiple processes of growth and development, particularly in diverse stress responses. Isatis indigotica is widely used in Traditional Chinese Medicine and is famous for its use as a dye for the color indigo. However, reports of the WRKY gene family in I. indigotica are limited. In this study, 64 IiWRKY genes encoding proteins with the complete WRKY domain were identified from genome of I. indigotica. Based on their structure and phylogenetic relationships of this gene family in I. indigotica, the IiWRKY genes were classified into three groups: Group I (n = 13), Group II (n = 35) and Group III (n = 16). Sequence alignment revealed that IiWRKY proteins harbored two variants, WRKYRQK and WRKYGKK, of the highly conserved WRKYGQK motif. The number of exons in IiWRKY genes varied from two to 14, with most of IiWRKY genes containing three exons. Investigation of gene duplication demonstrated that 10 and 14 IiWRKY genes were incorporated in tandem and segmental duplication events, respectively. Finally, the expression profiles derived from transcriptome data and quantitative real-time PCR analysis showed distinct expression patterns of these IiWRKY gene in five different organs or in response to four abiotic stresses. Taken together, our results will contribute to functional analysis of IiWRKY genes, and also provide a basis for further clarification of the molecular mechanism of stress responses in this important herb. SN - 1879-0038 UR - https://www.unboundmedicine.com/medline/citation/33705810/Identification_and_expression_analysis_of_the_WRKY_gene_family_in_Isatis_indigotica_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0378-1119(21)00155-4 DB - PRIME DP - Unbound Medicine ER -