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Extraction, purification, physicochemical properties and antioxidant activity of a new polysaccharide from Ocimum album L. seed.
Int J Biol Macromol. 2021 Jun 01; 180:643-653.IJ

Abstract

In this study, a novel polysaccharide fraction from Ocimum album seed was extracted and then purified by Cellulose DEAE-52 and Sephadex G-200 anion exchange chromatography. The structural, physicochemical and antioxidant properties of the main polysaccharide fraction (OAP-1A) were evaluated. The purified polysaccharide contained 94.3% carbohydrate, 3.56% moisture and 2.14% ash and result of gel permeation chromatography (GPC) showed average molecular weight of 593 kDa. The results of high-performance liquid chromatography (HPLC) showed that OAP-1A was a neutral hetero-polysaccharide composed of mannose (35.7%), glucose (33.32%), galactose (19.6%) and rhamnose (11.38%). In addition, GC-MS data, nuclear magnetic resonance (NMR) spectrum and Fourier transform infrared (FT-IR) analysis revealed that the backbone of OAP-1A consists of →3)-β-D-Manp-(1→, →3,4)-β-D-Manp-(1→, →3,6)-β-D-Manp-(1→, →3)-α-D-Glcp-(1→, →6)-β-D-Galp-(1→, →4)-α-L-Rhap-(1→ and α-D-Glcp-(1→. X-ray diffraction (XRD) analysis showed semi-crystalline structure in OAP-1A. Differential scanning colorimeter (DSC) and thermo-gravimetry analysis (TGA) indicated that OAP-1A had relatively high thermal stability. Moreover, OAP-1A showed strong scavenging activity against 2,2-diphenyl-1-picrylhydrazyl (DPPH) radicals.

Authors+Show Affiliations

Department of Food Science and Technology, Faculty of Agriculture, University of Tabriz, P.O. Box 51666-16471, Tabriz, Iran.Department of Food Science and Technology, Faculty of Agriculture, University of Tabriz, P.O. Box 51666-16471, Tabriz, Iran; Department of Food Engineering, Faculty of Engineering, Near East University, P. O. Box 99138, Nicosia, Cyprus, Mersin 10, Turkey. Electronic address: Ghanbarzadeh@tabrizu.ac.ir.Department of Food Science and Technology, Faculty of Agriculture, University of Tabriz, P.O. Box 51666-16471, Tabriz, Iran.Department of Food Science and Technology, Faculty of Agriculture Engineering, Shahrood University of Technology, P.O. Box 361999-5161, Shahrood, Iran.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

33744248

Citation

Arab, Khaled, et al. "Extraction, Purification, Physicochemical Properties and Antioxidant Activity of a New Polysaccharide From Ocimum Album L. Seed." International Journal of Biological Macromolecules, vol. 180, 2021, pp. 643-653.
Arab K, Ghanbarzadeh B, Ayaseh A, et al. Extraction, purification, physicochemical properties and antioxidant activity of a new polysaccharide from Ocimum album L. seed. Int J Biol Macromol. 2021;180:643-653.
Arab, K., Ghanbarzadeh, B., Ayaseh, A., & Jahanbin, K. (2021). Extraction, purification, physicochemical properties and antioxidant activity of a new polysaccharide from Ocimum album L. seed. International Journal of Biological Macromolecules, 180, 643-653. https://doi.org/10.1016/j.ijbiomac.2021.03.088
Arab K, et al. Extraction, Purification, Physicochemical Properties and Antioxidant Activity of a New Polysaccharide From Ocimum Album L. Seed. Int J Biol Macromol. 2021 Jun 1;180:643-653. PubMed PMID: 33744248.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Extraction, purification, physicochemical properties and antioxidant activity of a new polysaccharide from Ocimum album L. seed. AU - Arab,Khaled, AU - Ghanbarzadeh,Babak, AU - Ayaseh,Ali, AU - Jahanbin,Kambiz, Y1 - 2021/03/18/ PY - 2021/01/09/received PY - 2021/03/10/revised PY - 2021/03/15/accepted PY - 2021/3/22/pubmed PY - 2021/7/27/medline PY - 2021/3/21/entrez KW - (1)H and (13)C NMR KW - Ocimum album seed KW - Polysaccharide KW - Purification SP - 643 EP - 653 JF - International journal of biological macromolecules JO - Int J Biol Macromol VL - 180 N2 - In this study, a novel polysaccharide fraction from Ocimum album seed was extracted and then purified by Cellulose DEAE-52 and Sephadex G-200 anion exchange chromatography. The structural, physicochemical and antioxidant properties of the main polysaccharide fraction (OAP-1A) were evaluated. The purified polysaccharide contained 94.3% carbohydrate, 3.56% moisture and 2.14% ash and result of gel permeation chromatography (GPC) showed average molecular weight of 593 kDa. The results of high-performance liquid chromatography (HPLC) showed that OAP-1A was a neutral hetero-polysaccharide composed of mannose (35.7%), glucose (33.32%), galactose (19.6%) and rhamnose (11.38%). In addition, GC-MS data, nuclear magnetic resonance (NMR) spectrum and Fourier transform infrared (FT-IR) analysis revealed that the backbone of OAP-1A consists of →3)-β-D-Manp-(1→, →3,4)-β-D-Manp-(1→, →3,6)-β-D-Manp-(1→, →3)-α-D-Glcp-(1→, →6)-β-D-Galp-(1→, →4)-α-L-Rhap-(1→ and α-D-Glcp-(1→. X-ray diffraction (XRD) analysis showed semi-crystalline structure in OAP-1A. Differential scanning colorimeter (DSC) and thermo-gravimetry analysis (TGA) indicated that OAP-1A had relatively high thermal stability. Moreover, OAP-1A showed strong scavenging activity against 2,2-diphenyl-1-picrylhydrazyl (DPPH) radicals. SN - 1879-0003 UR - https://www.unboundmedicine.com/medline/citation/33744248/Extraction_purification_physicochemical_properties_and_antioxidant_activity_of_a_new_polysaccharide_from_Ocimum_album_L__seed_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0141-8130(21)00606-1 DB - PRIME DP - Unbound Medicine ER -