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Rapid SARS-CoV-2 antigen detection potentiates early diagnosis of COVID-19 disease.
Biosci Trends. 2021 May 11; 15(2):93-99.BT

Abstract

As the COVID-19 epidemic is still ongoing, a more rapid detection of SARS-CoV-2 infection such as viral antigen-detection needs to be evaluated for early diagnosis of COVID-19 disease. Here, we report the dynamic changes of SARS-CoV-2 viral antigens in nasopharyngeal swabs of COVID-19 patients and its association with the viral nucleic acid clearance and clinical outcomes. Eighty-five COVID-19 patients were enrolled for detection of SARS-CoV-2 viral antigens, including 57 anti-SARS-CoV-2 antibody negative cases and 28 antibody positive cases. The viral antigen could be detected in 52.63% (30/57) patients with SARS-CoV-2 antibody negative at the early stage of SARS-CoV-2 infection, especially in the first 5 days after disease onset (p = 0.0018) and disappeared in about 8 days after disease onset. Viral antigens were highly detectable in patients with low Ct value (less than 30) of SARS-CoV-2 nucleic acid RT-PCT assay, suggesting the expression of viral antigen was associated with high viral load. Furthermore, positive antigen detection indicated disease progression, nine cases with positive antigen (9/30, 30.0%), in contrast to two cases (2/27, 7.40%) (p = 0.0444) with negative antigen, which progressed into severe disease. Thus, the viral antigens were persistent in early stages of infection when virus was in highly replicating status, and viral antigen detection promises to rapidly screen positive patients in the early stage of SARS-CoV-2 infection.

Authors+Show Affiliations

Department of Integrative Medicine, Shanghai Public Health Clinical Center, Fudan University, Shanghai, China.Department of Drug Clinical Trial, Shanghai Public Health Clinical Center, Fudan University, Shanghai, China.Department of Surgery, Shanghai Public Health Clinical Center, Fudan University, Shanghai, China.Department of Pediatrics, Shanghai Public Health Clinical Center, Fudan University, Shanghai, China.Department of Nursing, Shanghai Public Health Clinical Center, Fudan University, Shanghai, China.Department of Pain Rehabilitation, Shanghai Public Health Clinical Center, Fudan University, Shanghai, China.Scientific Department, Shanghai Public Health Clinical Center, Fudan University, Shanghai, China.Scientific Department, Shanghai Public Health Clinical Center, Fudan University, Shanghai, China.Scientific Department, Shanghai Public Health Clinical Center, Fudan University, Shanghai, China.Department of Infectious Disease, Shanghai Public Health Clinical Center, Shanghai, China.Department of Infectious Disease, Shanghai Public Health Clinical Center, Shanghai, China.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

33776018

Citation

Lv, Ying, et al. "Rapid SARS-CoV-2 Antigen Detection Potentiates Early Diagnosis of COVID-19 Disease." Bioscience Trends, vol. 15, no. 2, 2021, pp. 93-99.
Lv Y, Ma Y, Si Y, et al. Rapid SARS-CoV-2 antigen detection potentiates early diagnosis of COVID-19 disease. Biosci Trends. 2021;15(2):93-99.
Lv, Y., Ma, Y., Si, Y., Zhu, X., Zhang, L., Feng, H., Tian, D., Liao, Y., Liu, T., Lu, H., & Ling, Y. (2021). Rapid SARS-CoV-2 antigen detection potentiates early diagnosis of COVID-19 disease. Bioscience Trends, 15(2), 93-99. https://doi.org/10.5582/bst.2021.01090
Lv Y, et al. Rapid SARS-CoV-2 Antigen Detection Potentiates Early Diagnosis of COVID-19 Disease. Biosci Trends. 2021 May 11;15(2):93-99. PubMed PMID: 33776018.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Rapid SARS-CoV-2 antigen detection potentiates early diagnosis of COVID-19 disease. AU - Lv,Ying, AU - Ma,Yuanyuan, AU - Si,Yanhui, AU - Zhu,Xiaoyi, AU - Zhang,Lin, AU - Feng,Haiyan, AU - Tian,Di, AU - Liao,Yixin, AU - Liu,Tiefu, AU - Lu,Hongzhou, AU - Ling,Yun, Y1 - 2021/03/26/ PY - 2021/3/30/pubmed PY - 2021/5/22/medline PY - 2021/3/29/entrez KW - COVID-19 KW - RT-qPCR Ct value KW - antigen-detection KW - nasopharyngeal swab virus nucleic acid KW - radiographic progression SP - 93 EP - 99 JF - Bioscience trends JO - Biosci Trends VL - 15 IS - 2 N2 - As the COVID-19 epidemic is still ongoing, a more rapid detection of SARS-CoV-2 infection such as viral antigen-detection needs to be evaluated for early diagnosis of COVID-19 disease. Here, we report the dynamic changes of SARS-CoV-2 viral antigens in nasopharyngeal swabs of COVID-19 patients and its association with the viral nucleic acid clearance and clinical outcomes. Eighty-five COVID-19 patients were enrolled for detection of SARS-CoV-2 viral antigens, including 57 anti-SARS-CoV-2 antibody negative cases and 28 antibody positive cases. The viral antigen could be detected in 52.63% (30/57) patients with SARS-CoV-2 antibody negative at the early stage of SARS-CoV-2 infection, especially in the first 5 days after disease onset (p = 0.0018) and disappeared in about 8 days after disease onset. Viral antigens were highly detectable in patients with low Ct value (less than 30) of SARS-CoV-2 nucleic acid RT-PCT assay, suggesting the expression of viral antigen was associated with high viral load. Furthermore, positive antigen detection indicated disease progression, nine cases with positive antigen (9/30, 30.0%), in contrast to two cases (2/27, 7.40%) (p = 0.0444) with negative antigen, which progressed into severe disease. Thus, the viral antigens were persistent in early stages of infection when virus was in highly replicating status, and viral antigen detection promises to rapidly screen positive patients in the early stage of SARS-CoV-2 infection. SN - 1881-7823 UR - https://www.unboundmedicine.com/medline/citation/33776018/Rapid_SARS_CoV_2_antigen_detection_potentiates_early_diagnosis_of_COVID_19_disease_ L2 - https://dx.doi.org/10.5582/bst.2021.01090 DB - PRIME DP - Unbound Medicine ER -