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Modulatory effect of methanol extract of Annona muricata stem bark on mitochondrial membrane permeability transition pore in normal rat liver and monosodium glutamate-induced uterine hyperplasia.
J Complement Integr Med. 2021 Mar 31; 18(2):355-361.JC

Abstract

OBJECTIVES

Uterine fibroids are benign tumors that develop in many women of reproductive age. Surgery is the main approach to treatment while other options are also associated with adverse effects. Studies have shown that certain bioactive agents present in medicinal plants elicit their anti-tumor activity by induction of mitochondrial permeability transition (mPT) opening. This research therefore aimed at investigating the effect of methanol extract of Annona muricata (MEAM) on mPT pore opening in normal and monosodium glutamate-induced uterine hyperplasia using female Wistar rats.

METHODS

Mitochondria, isolated from rat liver were exposed to different concentrations (20, 60, 100, 140 and 180 μg/mL) of MEAM. The mPT pore opening, cytochrome c release, mitochondrial ATPase (mATPase) activity and the percentage lipid peroxidation were assessed spectrophotometrically. Histological effects of MEAM on the liver, brain and uterus of normal and MSG-treated rats were investigated.

RESULTS

The in vitro results showed a significant induction of mPT pore opening by 2.4, 4.2 and 6.4 folds, release of cytochrome c and enhancement of mATPase activity at 100,140 and 180 μg/mL, respectively. However, oral administration of MEAM did not induce mPT pore opening, neither any significant release of cytochrome c nor enhancement of mATPase activity at all the dosages used. However, histological assay revealed the presence of MSG-induced cellular damage and uterine hyperplasia which was ameliorated by MEAM co-administration.

CONCLUSIONS

These findings suggest that MEAM contains phytochemicals that can ameliorate MSG-induced damage and uterine hyperplasia in rats; however, the mechanism might not be via upregulation of mitochondrial-mediated apoptosis.

Authors+Show Affiliations

Department of Biochemistry, Faculty of Basic Medical Sciences, College of Medicine, University of Ibadan, Ibadan, Nigeria.Department of Biochemistry, Faculty of Basic Medical Sciences, College of Medicine, University of Ibadan, Ibadan, Nigeria.Department of Biochemistry, Faculty of Basic Medical Sciences, College of Medicine, University of Ibadan, Ibadan, Nigeria.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

33787189

Citation

Olowofolahan, Adeola Oluwakemi, et al. "Modulatory Effect of Methanol Extract of Annona Muricata Stem Bark On Mitochondrial Membrane Permeability Transition Pore in Normal Rat Liver and Monosodium Glutamate-induced Uterine Hyperplasia." Journal of Complementary & Integrative Medicine, vol. 18, no. 2, 2021, pp. 355-361.
Olowofolahan AO, Adewoye FO, Olorunsogo OO. Modulatory effect of methanol extract of Annona muricata stem bark on mitochondrial membrane permeability transition pore in normal rat liver and monosodium glutamate-induced uterine hyperplasia. J Complement Integr Med. 2021;18(2):355-361.
Olowofolahan, A. O., Adewoye, F. O., & Olorunsogo, O. O. (2021). Modulatory effect of methanol extract of Annona muricata stem bark on mitochondrial membrane permeability transition pore in normal rat liver and monosodium glutamate-induced uterine hyperplasia. Journal of Complementary & Integrative Medicine, 18(2), 355-361. https://doi.org/10.1515/jcim-2019-0341
Olowofolahan AO, Adewoye FO, Olorunsogo OO. Modulatory Effect of Methanol Extract of Annona Muricata Stem Bark On Mitochondrial Membrane Permeability Transition Pore in Normal Rat Liver and Monosodium Glutamate-induced Uterine Hyperplasia. J Complement Integr Med. 2021 Mar 31;18(2):355-361. PubMed PMID: 33787189.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Modulatory effect of methanol extract of Annona muricata stem bark on mitochondrial membrane permeability transition pore in normal rat liver and monosodium glutamate-induced uterine hyperplasia. AU - Olowofolahan,Adeola Oluwakemi, AU - Adewoye,Funmilayo O, AU - Olorunsogo,Olufunso Olabode, Y1 - 2021/03/31/ PY - 2020/02/12/received PY - 2020/07/09/accepted PY - 2021/4/1/pubmed PY - 2021/4/1/medline PY - 2021/3/31/entrez KW - Annona muricata KW - apoptosis KW - mitochondrial membrane permeability transition pore KW - monosodium glutamate KW - uterine hyperplasia SP - 355 EP - 361 JF - Journal of complementary & integrative medicine JO - J Complement Integr Med VL - 18 IS - 2 N2 - OBJECTIVES: Uterine fibroids are benign tumors that develop in many women of reproductive age. Surgery is the main approach to treatment while other options are also associated with adverse effects. Studies have shown that certain bioactive agents present in medicinal plants elicit their anti-tumor activity by induction of mitochondrial permeability transition (mPT) opening. This research therefore aimed at investigating the effect of methanol extract of Annona muricata (MEAM) on mPT pore opening in normal and monosodium glutamate-induced uterine hyperplasia using female Wistar rats. METHODS: Mitochondria, isolated from rat liver were exposed to different concentrations (20, 60, 100, 140 and 180 μg/mL) of MEAM. The mPT pore opening, cytochrome c release, mitochondrial ATPase (mATPase) activity and the percentage lipid peroxidation were assessed spectrophotometrically. Histological effects of MEAM on the liver, brain and uterus of normal and MSG-treated rats were investigated. RESULTS: The in vitro results showed a significant induction of mPT pore opening by 2.4, 4.2 and 6.4 folds, release of cytochrome c and enhancement of mATPase activity at 100,140 and 180 μg/mL, respectively. However, oral administration of MEAM did not induce mPT pore opening, neither any significant release of cytochrome c nor enhancement of mATPase activity at all the dosages used. However, histological assay revealed the presence of MSG-induced cellular damage and uterine hyperplasia which was ameliorated by MEAM co-administration. CONCLUSIONS: These findings suggest that MEAM contains phytochemicals that can ameliorate MSG-induced damage and uterine hyperplasia in rats; however, the mechanism might not be via upregulation of mitochondrial-mediated apoptosis. SN - 1553-3840 UR - https://www.unboundmedicine.com/medline/citation/33787189/Modulatory_effect_of_methanol_extract_of_Annona_muricata_stem_bark_on_mitochondrial_membrane_permeability_transition_pore_in_normal_rat_liver_and_monosodium_glutamate_induced_uterine_hyperplasia_ L2 - https://www.degruyter.com/document/doi/10.1515/jcim-2019-0341 DB - PRIME DP - Unbound Medicine ER -