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Development of Novel Wheat-Aegilops longissima 3S1 Translocations Conferring Powdery Mildew Resistance and Specific Molecular Markers for Chromosome 3S1.
Plant Dis. 2021 Oct; 105(10):2938-2945.PD

Abstract

Powdery mildew of wheat, caused by Blumeria graminis f. sp. tritici, is a destructive disease of common wheat. Cultivation of resistant varieties is the most cost-effective disease management strategy. Previous studies reported that chromosome 3Sl#2 present in Chinese Spring (CS)-Aegilops longissima 3Sl#2(3B) disomic substitution line TA3575 conferred resistance to powdery mildew. In this study, we further located the powdery mildew resistance gene(s) to the short arm of chromosome 3Sl#2 (3Sl#2S) by evaluating for B. graminis f. sp. tritici resistance of newly developed CS-Ae. longissima 3Sl#2 translocation lines. Meanwhile, TA7545, a previously designated CS-Ae. longissima 3Sl#3 disomic addition line, was reidentified as an isochromosome 3Sl#3S addition line and evaluated to confer resistance to powdery mildew, thus locating the resistance gene(s) to the short arm of chromosome 3Sl#3 (3Sl#3S). Based on transcriptome sequences of TA3575, 10 novel chromosome 3SlS-specific markers were developed, of which 5 could be used to distinguish between 3Sl#2S and 3Sl#3S derived from Ae. longissima accessions TL20 and TA1910 (TAM4) and the remaining 5 could identify both 3Sl#2S and 3Sl#3S. Also, CL897, one of five markers specific to both 3Sl#2S and 3Sl#3S, could be used to detect Pm13 located at chromosome 3Sl#1S from Ae. longissima accession TL01 in diverse wheat genetic backgrounds. The powdery mildew resistance genes on chromosomes 3Sl#2S and 3Sl#3S, the CS-Ae. longissima 3Sl#2 translocation lines, and the 3SlS-specific markers developed in this study will facilitate the transfer of B. graminis f. sp. tritici resistance genes into common wheat and provide new germplasm resources for powdery mildew resistance breeding.

Authors+Show Affiliations

College of Life Sciences, Henan Agricultural University, Zhengzhou 450002, China.College of Life Sciences, Henan Agricultural University, Zhengzhou 450002, China.College of Life Sciences, Henan Agricultural University, Zhengzhou 450002, China.College of Life Sciences, Henan Agricultural University, Zhengzhou 450002, China.College of Life Sciences, Henan Agricultural University, Zhengzhou 450002, China.Department of Agronomy, Horticulture and Plant Science, South Dakota State University, Brookings, SD 57007 U.S.A.College of Life Sciences, Henan Agricultural University, Zhengzhou 450002, China.College of Life Sciences, Henan Agricultural University, Zhengzhou 450002, China.College of Life Sciences, Henan Agricultural University, Zhengzhou 450002, China.College of Life Sciences, Henan Agricultural University, Zhengzhou 450002, China.Henan Academy of Agricultural Sciences, Zhengzhou 450002, China.Wheat Genetic and Genomic Resources Center, Department of Plant Pathology, Throckmorton Plant Sciences Center, Kansas State University, Manhattan, KS 66506 U.S.A.College of Life Sciences, Henan Agricultural University, Zhengzhou 450002, China.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

33787309

Citation

Li, Huanhuan, et al. "Development of Novel Wheat-Aegilops Longissima 3S1 Translocations Conferring Powdery Mildew Resistance and Specific Molecular Markers for Chromosome 3S1." Plant Disease, vol. 105, no. 10, 2021, pp. 2938-2945.
Li H, Tian X, Pei S, et al. Development of Novel Wheat-Aegilops longissima 3S1 Translocations Conferring Powdery Mildew Resistance and Specific Molecular Markers for Chromosome 3S1. Plant Dis. 2021;105(10):2938-2945.
Li, H., Tian, X., Pei, S., Men, W., Ma, C., Sehgal, S. K., Zhao, Y., Chen, Q., Wang, B., Dong, Z., Xiang, Z., Friebe, B., & Liu, W. (2021). Development of Novel Wheat-Aegilops longissima 3S1 Translocations Conferring Powdery Mildew Resistance and Specific Molecular Markers for Chromosome 3S1. Plant Disease, 105(10), 2938-2945. https://doi.org/10.1094/PDIS-12-20-2691-RE
Li H, et al. Development of Novel Wheat-Aegilops Longissima 3S1 Translocations Conferring Powdery Mildew Resistance and Specific Molecular Markers for Chromosome 3S1. Plant Dis. 2021;105(10):2938-2945. PubMed PMID: 33787309.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Development of Novel Wheat-Aegilops longissima 3S1 Translocations Conferring Powdery Mildew Resistance and Specific Molecular Markers for Chromosome 3S1. AU - Li,Huanhuan, AU - Tian,Xiubin, AU - Pei,Shaolong, AU - Men,Wenqiang, AU - Ma,Chao, AU - Sehgal,Sunish K, AU - Zhao,Yue, AU - Chen,Qifan, AU - Wang,Beilin, AU - Dong,Zhenjie, AU - Xiang,Zhiguo, AU - Friebe,Bernd, AU - Liu,Wenxuan, Y1 - 2021/10/26/ PY - 2021/4/1/pubmed PY - 2021/12/31/medline PY - 2021/3/31/entrez KW - 3S1 KW - Aegilops longissima KW - common wheat KW - molecular markers KW - powdery mildew resistance SP - 2938 EP - 2945 JF - Plant disease JO - Plant Dis VL - 105 IS - 10 N2 - Powdery mildew of wheat, caused by Blumeria graminis f. sp. tritici, is a destructive disease of common wheat. Cultivation of resistant varieties is the most cost-effective disease management strategy. Previous studies reported that chromosome 3Sl#2 present in Chinese Spring (CS)-Aegilops longissima 3Sl#2(3B) disomic substitution line TA3575 conferred resistance to powdery mildew. In this study, we further located the powdery mildew resistance gene(s) to the short arm of chromosome 3Sl#2 (3Sl#2S) by evaluating for B. graminis f. sp. tritici resistance of newly developed CS-Ae. longissima 3Sl#2 translocation lines. Meanwhile, TA7545, a previously designated CS-Ae. longissima 3Sl#3 disomic addition line, was reidentified as an isochromosome 3Sl#3S addition line and evaluated to confer resistance to powdery mildew, thus locating the resistance gene(s) to the short arm of chromosome 3Sl#3 (3Sl#3S). Based on transcriptome sequences of TA3575, 10 novel chromosome 3SlS-specific markers were developed, of which 5 could be used to distinguish between 3Sl#2S and 3Sl#3S derived from Ae. longissima accessions TL20 and TA1910 (TAM4) and the remaining 5 could identify both 3Sl#2S and 3Sl#3S. Also, CL897, one of five markers specific to both 3Sl#2S and 3Sl#3S, could be used to detect Pm13 located at chromosome 3Sl#1S from Ae. longissima accession TL01 in diverse wheat genetic backgrounds. The powdery mildew resistance genes on chromosomes 3Sl#2S and 3Sl#3S, the CS-Ae. longissima 3Sl#2 translocation lines, and the 3SlS-specific markers developed in this study will facilitate the transfer of B. graminis f. sp. tritici resistance genes into common wheat and provide new germplasm resources for powdery mildew resistance breeding. SN - 0191-2917 UR - https://www.unboundmedicine.com/medline/citation/33787309/Development_of_Novel_Wheat_Aegilops_longissima_3S1_Translocations_Conferring_Powdery_Mildew_Resistance_and_Specific_Molecular_Markers_for_Chromosome_3S1_ DB - PRIME DP - Unbound Medicine ER -