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High-viability lyophilized Bacille Calmette-Guérin vaccine produced by deep-culture technique.
Appl Environ Microbiol 1977; 34(5):495-9AE

Abstract

Evidence suggests that Bacille Calmette-Guérin (BCG) vaccine for use in cancer immunotherapy should have the following characteristics: high viability which is maintained on storage; high ratio of live to dead cells; high proportion of single cells; and low content of soluble antigen. The production of a vaccine with these characteristics was accomplished by use of a deep-culture technique. The medium was modified Proskauer and Beck medium containing Tween 80 and glucose. The mass culture was grown in a Wheaton double-side-arm bottle (6 liters of medium in an 8-liter container), aerated by means of an aquarium aerator and mixed by a magnetic stirrer. The culture was incubated 7 to 9 days at 37 degrees C, concentrated 11 to 15 times by ultrafiltration, diluted with equal parts of 25% lactose, and then lyophilized. The lyophilized ampoules, stored at -70 degrees C, were cultured at intervals ranging from 3 days to 450 days, and no loss in viability was observed. The mean number of viable BCG per ml of reconstituted vaccine was 8.75 log10. The viable count was 90% of the total bacterial count. Moreover, 85% of the cells were present as single bacilli.

Authors

No affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

337895

Citation

Kim, T H.. "High-viability Lyophilized Bacille Calmette-Guérin Vaccine Produced By Deep-culture Technique." Applied and Environmental Microbiology, vol. 34, no. 5, 1977, pp. 495-9.
Kim TH. High-viability lyophilized Bacille Calmette-Guérin vaccine produced by deep-culture technique. Appl Environ Microbiol. 1977;34(5):495-9.
Kim, T. H. (1977). High-viability lyophilized Bacille Calmette-Guérin vaccine produced by deep-culture technique. Applied and Environmental Microbiology, 34(5), pp. 495-9.
Kim TH. High-viability Lyophilized Bacille Calmette-Guérin Vaccine Produced By Deep-culture Technique. Appl Environ Microbiol. 1977;34(5):495-9. PubMed PMID: 337895.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - High-viability lyophilized Bacille Calmette-Guérin vaccine produced by deep-culture technique. A1 - Kim,T H, PY - 1977/11/1/pubmed PY - 1977/11/1/medline PY - 1977/11/1/entrez SP - 495 EP - 9 JF - Applied and environmental microbiology JO - Appl. Environ. Microbiol. VL - 34 IS - 5 N2 - Evidence suggests that Bacille Calmette-Guérin (BCG) vaccine for use in cancer immunotherapy should have the following characteristics: high viability which is maintained on storage; high ratio of live to dead cells; high proportion of single cells; and low content of soluble antigen. The production of a vaccine with these characteristics was accomplished by use of a deep-culture technique. The medium was modified Proskauer and Beck medium containing Tween 80 and glucose. The mass culture was grown in a Wheaton double-side-arm bottle (6 liters of medium in an 8-liter container), aerated by means of an aquarium aerator and mixed by a magnetic stirrer. The culture was incubated 7 to 9 days at 37 degrees C, concentrated 11 to 15 times by ultrafiltration, diluted with equal parts of 25% lactose, and then lyophilized. The lyophilized ampoules, stored at -70 degrees C, were cultured at intervals ranging from 3 days to 450 days, and no loss in viability was observed. The mean number of viable BCG per ml of reconstituted vaccine was 8.75 log10. The viable count was 90% of the total bacterial count. Moreover, 85% of the cells were present as single bacilli. SN - 0099-2240 UR - https://www.unboundmedicine.com/medline/citation/337895/High_viability_lyophilized_Bacille_Calmette_Guérin_vaccine_produced_by_deep_culture_technique_ L2 - http://aem.asm.org/cgi/pmidlookup?view=long&pmid=337895 DB - PRIME DP - Unbound Medicine ER -