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[Effect of proximal and distal acupoint catgut-embedding on uterus prostaglandin, serum IL-2 and splenic NK cell activity in primary dysmenorrhea rats].
Zhen Ci Yan Jiu. 2021 Mar 25; 46(3):221-5.ZC

Abstract

OBJECTIVE

To observe the effect of proximal and distal acupoint catgut-embedding on the contents of prostaglandin E2 (PGE2) and prostaglandin F2α(PGF2α) in the uterus, serum Interleukin-2 (IL-2) contents and splenic natural killer (NK) cell activity in primary dysmenorrhea (PD) rats,so as to explore the different effects of proximal and distal acupoint catgut-embedding on PD rats.

METHODS

Female Wistar rats were randomly divided into control, model, proximal catgut-embedding and distal catgut-embedding groups, with 8 rats in each group. The PD model was established by subcutaneous injection of estradiol ben-zoate (0.5 mg/rat on the first day and 10th day, and 0.2 mg/rat from the 2nd to the 9th day) and intraperitoneal injection of oxytocin (2 U/rat, on the 11th day). Catgut-embedding was applied at bilateral "Guanyuan"(CV4) and "Ciliao"(BL32) in the proximal catgut-embedding group, and bilateral "Sanyinjiao" (SP6) and "Diji"(SP8) in the distal catgut-embedding group. After the treatment, the body writhing times in 30 min and uterine mass were recorded, PGE2 and PGF2αin uterus and IL-2 in serum were assayed by using ELISA, the activity of NK cell in the spleen was detected using MTT colorimetry.

RESULTS

Following modeling, the body writhing times of the model group was increased than that of the control group (P<0.01); After interventions, the body writhing times were decreased in the proximal and distal catgut-embedding groups than those of the model group (P<0.01). Compared with the control group, the uterine mass and uterus PGF2α content were increased (P<0.01), while uterus PGE2, serum IL-2 and splenic NK cell activity were decreased (P<0.01) in the model group. After interventions, the uterine mass and uterus PGF2α were down-regulated (P<0.01, P<0.05), while the contents of uterus PGE2, serum IL-2 and splenic NK cell activity up-regulated (P<0.01) in the proximal and distal catgut-embedding groups in contrast to the model group. The content of uterus PGF2α was down-regulated (P<0.05) and splenic NK cell activity was up-regulated (P<0.01) in the proximal catgut-embedding group than those of the distal catgut-embedding group.

CONCLUSION

Both proximal and distal acupoint catgut-embeding can increase PGE2 and decrease PGF2α in the uterus, increase the level of serum IL-2 and the activity of NK cells in the spleen in PD model rats, thereby achieving analgesic effect. The effect of proximal catgut-embedding is better than that of distal catgut-embedding.

Authors+Show Affiliations

Graduate School of Guizhou University of Traditional Chinese Medicine, Guiyang 550000, China.College of Acupuncture and Orthopedics, Guizhou University of Traditional Chinese Medicine, Guiyang 550002.College of Acupuncture and Orthopedics, Guizhou University of Traditional Chinese Medicine, Guiyang 550002.College of Acupuncture and Orthopedics, Guizhou University of Traditional Chinese Medicine, Guiyang 550002.Graduate School of Guizhou University of Traditional Chinese Medicine, Guiyang 550000, China.Graduate School of Guizhou University of Traditional Chinese Medicine, Guiyang 550000, China.Graduate School of Guizhou University of Traditional Chinese Medicine, Guiyang 550000, China.

Pub Type(s)

Journal Article

Language

chi

PubMed ID

33798295

Citation

Yang, Wen-Wen, et al. "[Effect of Proximal and Distal Acupoint Catgut-embedding On Uterus Prostaglandin, Serum IL-2 and Splenic NK Cell Activity in Primary Dysmenorrhea Rats]." Zhen Ci Yan Jiu = Acupuncture Research, vol. 46, no. 3, 2021, pp. 221-5.
Yang WW, Chen PB, Jin LM, et al. [Effect of proximal and distal acupoint catgut-embedding on uterus prostaglandin, serum IL-2 and splenic NK cell activity in primary dysmenorrhea rats]. Zhen Ci Yan Jiu. 2021;46(3):221-5.
Yang, W. W., Chen, P. B., Jin, L. M., Tang, X. Y., Qin, Z. Y., Zhou, Y. J., & Chen, Y. (2021). [Effect of proximal and distal acupoint catgut-embedding on uterus prostaglandin, serum IL-2 and splenic NK cell activity in primary dysmenorrhea rats]. Zhen Ci Yan Jiu = Acupuncture Research, 46(3), 221-5. https://doi.org/10.13702/j.1000-0607.200618
Yang WW, et al. [Effect of Proximal and Distal Acupoint Catgut-embedding On Uterus Prostaglandin, Serum IL-2 and Splenic NK Cell Activity in Primary Dysmenorrhea Rats]. Zhen Ci Yan Jiu. 2021 Mar 25;46(3):221-5. PubMed PMID: 33798295.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Effect of proximal and distal acupoint catgut-embedding on uterus prostaglandin, serum IL-2 and splenic NK cell activity in primary dysmenorrhea rats]. AU - Yang,Wen-Wen, AU - Chen,Pan-Bi, AU - Jin,Ling-Min, AU - Tang,Xu-Yun, AU - Qin,Zhong-Yin, AU - Zhou,Yang-Jiaqi, AU - Chen,Yi, PY - 2021/4/2/entrez PY - 2021/4/3/pubmed PY - 2021/4/7/medline KW - Distal catgut-embedding KW - Primary dysmenorrhea KW - Prostaglandin E2 KW - Prostaglandin F2α KW - Proximal catgut-embedding KW - Splenic NK cell activity SP - 221 EP - 5 JF - Zhen ci yan jiu = Acupuncture research JO - Zhen Ci Yan Jiu VL - 46 IS - 3 N2 - OBJECTIVE: To observe the effect of proximal and distal acupoint catgut-embedding on the contents of prostaglandin E2 (PGE2) and prostaglandin F2α(PGF2α) in the uterus, serum Interleukin-2 (IL-2) contents and splenic natural killer (NK) cell activity in primary dysmenorrhea (PD) rats,so as to explore the different effects of proximal and distal acupoint catgut-embedding on PD rats. METHODS: Female Wistar rats were randomly divided into control, model, proximal catgut-embedding and distal catgut-embedding groups, with 8 rats in each group. The PD model was established by subcutaneous injection of estradiol ben-zoate (0.5 mg/rat on the first day and 10th day, and 0.2 mg/rat from the 2nd to the 9th day) and intraperitoneal injection of oxytocin (2 U/rat, on the 11th day). Catgut-embedding was applied at bilateral "Guanyuan"(CV4) and "Ciliao"(BL32) in the proximal catgut-embedding group, and bilateral "Sanyinjiao" (SP6) and "Diji"(SP8) in the distal catgut-embedding group. After the treatment, the body writhing times in 30 min and uterine mass were recorded, PGE2 and PGF2αin uterus and IL-2 in serum were assayed by using ELISA, the activity of NK cell in the spleen was detected using MTT colorimetry. RESULTS: Following modeling, the body writhing times of the model group was increased than that of the control group (P<0.01); After interventions, the body writhing times were decreased in the proximal and distal catgut-embedding groups than those of the model group (P<0.01). Compared with the control group, the uterine mass and uterus PGF2α content were increased (P<0.01), while uterus PGE2, serum IL-2 and splenic NK cell activity were decreased (P<0.01) in the model group. After interventions, the uterine mass and uterus PGF2α were down-regulated (P<0.01, P<0.05), while the contents of uterus PGE2, serum IL-2 and splenic NK cell activity up-regulated (P<0.01) in the proximal and distal catgut-embedding groups in contrast to the model group. The content of uterus PGF2α was down-regulated (P<0.05) and splenic NK cell activity was up-regulated (P<0.01) in the proximal catgut-embedding group than those of the distal catgut-embedding group. CONCLUSION: Both proximal and distal acupoint catgut-embeding can increase PGE2 and decrease PGF2α in the uterus, increase the level of serum IL-2 and the activity of NK cells in the spleen in PD model rats, thereby achieving analgesic effect. The effect of proximal catgut-embedding is better than that of distal catgut-embedding. SN - 1000-0607 UR - https://www.unboundmedicine.com/medline/citation/33798295/[Effect_of_proximal_and_distal_acupoint_catgut_embedding_on_uterus_prostaglandin_serum_IL_2_and_splenic_NK_cell_activity_in_primary_dysmenorrhea_rats]_ DB - PRIME DP - Unbound Medicine ER -