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Olive (Olea europaea L.) Genetic Transformation: Current Status and Future Prospects.
Genes (Basel). 2021 03 09; 12(3)G

Abstract

Olive (Olea europaea L.) is the most characteristic and important oil crop of the Mediterranean region. Traditional olive cultivation is based on few tens cultivars of ancient origin. To improve this crop, novel selections with higher tolerance to biotic and abiotic stress, adaptable to high-density planting systems and resilient to climate change are needed; however, breeding programs are hindered by the long juvenile period of this species and few improved genotypes have been released so far. Genetic transformation could be of great value, in the near future, to develop new varieties or rootstocks in a shorter time; in addition, it has currently become an essential tool for functional genomic studies. The recalcitrance of olive tissues to their in vitro manipulation has been the main bottleneck in the development of genetic transformation procedures in this species; however, some important traits such as fungal resistance, flowering or lipid composition have successfully been manipulated through the genetic transformation of somatic embryos of juvenile or adult origin, providing a proof of the potential role that this technology could have in olive improvement. However, the optimization of these protocols for explants of adult origin is a prerequisite to obtain useful materials for the olive industry. In this review, initially, factors affecting plant regeneration via somatic embryogenesis are discussed. Subsequently, the different transformation approaches explored in olive are reviewed. Finally, transgenic experiments with genes of interest undertaken to manipulate selected traits are discussed.

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Authors+Show Affiliations

Palomo-Ríos E
Instituto de Hortofruticultura Subtropical y Mediterránea "La Mayora", Departamento de Botánica y Fisiología Vegetal, Universidad de Málaga, 29071 Málaga, Spain.
Narváez I
Instituto de Hortofruticultura Subtropical y Mediterránea "La Mayora", Departamento de Botánica y Fisiología Vegetal, Universidad de Málaga, 29071 Málaga, Spain.
Pliego-Alfaro F
Instituto de Hortofruticultura Subtropical y Mediterránea "La Mayora", Departamento de Botánica y Fisiología Vegetal, Universidad de Málaga, 29071 Málaga, Spain.
Mercado JA
Instituto de Hortofruticultura Subtropical y Mediterránea "La Mayora", Departamento de Botánica y Fisiología Vegetal, Universidad de Málaga, 29071 Málaga, Spain.

MeSH

Genetic VariationGenome, PlantGenotypeOleaPhylogenyPlant BreedingRegenerationTransformation, Genetic

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't
Review

Language

eng

PubMed ID

33803172

Citation

Palomo-Ríos, Elena, et al. "Olive (Olea Europaea L.) Genetic Transformation: Current Status and Future Prospects." Genes, vol. 12, no. 3, 2021.
Palomo-Ríos E, Narváez I, Pliego-Alfaro F, et al. Olive (Olea europaea L.) Genetic Transformation: Current Status and Future Prospects. Genes (Basel). 2021;12(3).
Palomo-Ríos, E., Narváez, I., Pliego-Alfaro, F., & Mercado, J. A. (2021). Olive (Olea europaea L.) Genetic Transformation: Current Status and Future Prospects. Genes, 12(3). https://doi.org/10.3390/genes12030386
Palomo-Ríos E, et al. Olive (Olea Europaea L.) Genetic Transformation: Current Status and Future Prospects. Genes (Basel). 2021 03 9;12(3) PubMed PMID: 33803172.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Olive (Olea europaea L.) Genetic Transformation: Current Status and Future Prospects. AU - Palomo-Ríos,Elena, AU - Narváez,Isabel, AU - Pliego-Alfaro,Fernando, AU - Mercado,José A, Y1 - 2021/03/09/ PY - 2021/01/25/received PY - 2021/02/16/revised PY - 2021/03/03/accepted PY - 2021/4/3/entrez PY - 2021/4/4/pubmed PY - 2021/8/6/medline KW - Agrobacterium rhizogenes KW - Agrobacterium tumefaciens KW - biolistic KW - olive KW - somatic embryogenesis KW - transgenic plant JF - Genes JO - Genes (Basel) VL - 12 IS - 3 N2 - Olive (Olea europaea L.) is the most characteristic and important oil crop of the Mediterranean region. Traditional olive cultivation is based on few tens cultivars of ancient origin. To improve this crop, novel selections with higher tolerance to biotic and abiotic stress, adaptable to high-density planting systems and resilient to climate change are needed; however, breeding programs are hindered by the long juvenile period of this species and few improved genotypes have been released so far. Genetic transformation could be of great value, in the near future, to develop new varieties or rootstocks in a shorter time; in addition, it has currently become an essential tool for functional genomic studies. The recalcitrance of olive tissues to their in vitro manipulation has been the main bottleneck in the development of genetic transformation procedures in this species; however, some important traits such as fungal resistance, flowering or lipid composition have successfully been manipulated through the genetic transformation of somatic embryos of juvenile or adult origin, providing a proof of the potential role that this technology could have in olive improvement. However, the optimization of these protocols for explants of adult origin is a prerequisite to obtain useful materials for the olive industry. In this review, initially, factors affecting plant regeneration via somatic embryogenesis are discussed. Subsequently, the different transformation approaches explored in olive are reviewed. Finally, transgenic experiments with genes of interest undertaken to manipulate selected traits are discussed. SN - 2073-4425 UR - https://www.unboundmedicine.com/medline/citation/33803172/Olive__Olea_europaea_L___Genetic_Transformation:_Current_Status_and_Future_Prospects_ DB - PRIME DP - Unbound Medicine ER -