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Chemical Composition, Antioxidant and Cytoprotective Potentials of Carica papaya Leaf Extracts: A Comparison of Supercritical Fluid and Conventional Extraction Methods.
Molecules. 2021 Mar 09; 26(5)M

Abstract

The leaves of Carica papaya (CP) are rich in natural antioxidants. Carica papaya has traditionally been used to treat various ailments, including skin diseases. This study aims to decipher the antioxidant effects and phytochemical content of different CP leaf extracts (CPEs) obtained using supercritical carbon dioxide (scCO2) and conventional extraction methods. The antioxidant activities of CPEs were evaluated by cell-free (1,1-diphenyl-2-picryl-hydrazyl (DPPH) and ferric-reduced antioxidative power (FRAP)) and cell-based (H2O2) assay. Both C. papaya leaf scCO2 extract with 5% ethanol (CPSCE) and C. papaya leaf scCO2 extract (CPSC) exhibited stronger DPPH radical scavenging activity than conventional extracts. In the FRAP assay, two hydrophilic extracts (C. papaya leaf ethanol extract (CPEE) and C. papaya freeze-dried leaf juice (CPFD)) showed relatively stronger reducing power compared to lipophilic extracts. Cell-based assays showed that CPFD significantly protected skin fibroblasts from H2O2-induced oxidative stress in both pre-and post-treatment. CPEE protected skin fibroblasts from oxidative stress in a dose-dependent manner while CPSCE significantly triggered the fibroblast recovery after treatment with H2O2. GC-MS analysis indicated that CPSCE had the highest α-tocopherol and squalene contents. By contrast, both CP hydrophilic extracts (CPEE and CPFD) had a higher total phenolic content (TPC) and rutin content than the lipophilic extracts. Overall, CPEs extracted using green and conventional extraction methods showed antioxidative potential in both cell-based and cell-free assays due to their lipophilic and hydrophilic antioxidants, respectively.

Authors+Show Affiliations

School of Pharmaceutical Sciences, Universiti Sains Malaysia, Minden 11800, Penang, Malaysia.Centre for Drug Research, Universiti Sains Malaysia, Minden 11800, Penang, Malaysia.Centre for Drug Research, Universiti Sains Malaysia, Minden 11800, Penang, Malaysia.School of Pharmacy, Monash University Malaysia, Bandar Sunway 47500, Selangor Darul Ehsan, Malaysia.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

33803330

Citation

Khor, Boon-Keat, et al. "Chemical Composition, Antioxidant and Cytoprotective Potentials of Carica Papaya Leaf Extracts: a Comparison of Supercritical Fluid and Conventional Extraction Methods." Molecules (Basel, Switzerland), vol. 26, no. 5, 2021.
Khor BK, Chear NJ, Azizi J, et al. Chemical Composition, Antioxidant and Cytoprotective Potentials of Carica papaya Leaf Extracts: A Comparison of Supercritical Fluid and Conventional Extraction Methods. Molecules. 2021;26(5).
Khor, B. K., Chear, N. J., Azizi, J., & Khaw, K. Y. (2021). Chemical Composition, Antioxidant and Cytoprotective Potentials of Carica papaya Leaf Extracts: A Comparison of Supercritical Fluid and Conventional Extraction Methods. Molecules (Basel, Switzerland), 26(5). https://doi.org/10.3390/molecules26051489
Khor BK, et al. Chemical Composition, Antioxidant and Cytoprotective Potentials of Carica Papaya Leaf Extracts: a Comparison of Supercritical Fluid and Conventional Extraction Methods. Molecules. 2021 Mar 9;26(5) PubMed PMID: 33803330.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Chemical Composition, Antioxidant and Cytoprotective Potentials of Carica papaya Leaf Extracts: A Comparison of Supercritical Fluid and Conventional Extraction Methods. AU - Khor,Boon-Keat, AU - Chear,Nelson Jeng-Yeou, AU - Azizi,Juzaili, AU - Khaw,Kooi-Yeong, Y1 - 2021/03/09/ PY - 2021/01/15/received PY - 2021/02/21/revised PY - 2021/02/25/accepted PY - 2021/4/3/entrez PY - 2021/4/4/pubmed PY - 2021/5/14/medline KW - Carica papaya KW - antioxidant activities KW - co-solvent KW - hydrogen peroxide KW - rutin KW - skin fibroblast KW - squalene KW - supercritical carbon dioxide KW - α-tocopherol JF - Molecules (Basel, Switzerland) JO - Molecules VL - 26 IS - 5 N2 - The leaves of Carica papaya (CP) are rich in natural antioxidants. Carica papaya has traditionally been used to treat various ailments, including skin diseases. This study aims to decipher the antioxidant effects and phytochemical content of different CP leaf extracts (CPEs) obtained using supercritical carbon dioxide (scCO2) and conventional extraction methods. The antioxidant activities of CPEs were evaluated by cell-free (1,1-diphenyl-2-picryl-hydrazyl (DPPH) and ferric-reduced antioxidative power (FRAP)) and cell-based (H2O2) assay. Both C. papaya leaf scCO2 extract with 5% ethanol (CPSCE) and C. papaya leaf scCO2 extract (CPSC) exhibited stronger DPPH radical scavenging activity than conventional extracts. In the FRAP assay, two hydrophilic extracts (C. papaya leaf ethanol extract (CPEE) and C. papaya freeze-dried leaf juice (CPFD)) showed relatively stronger reducing power compared to lipophilic extracts. Cell-based assays showed that CPFD significantly protected skin fibroblasts from H2O2-induced oxidative stress in both pre-and post-treatment. CPEE protected skin fibroblasts from oxidative stress in a dose-dependent manner while CPSCE significantly triggered the fibroblast recovery after treatment with H2O2. GC-MS analysis indicated that CPSCE had the highest α-tocopherol and squalene contents. By contrast, both CP hydrophilic extracts (CPEE and CPFD) had a higher total phenolic content (TPC) and rutin content than the lipophilic extracts. Overall, CPEs extracted using green and conventional extraction methods showed antioxidative potential in both cell-based and cell-free assays due to their lipophilic and hydrophilic antioxidants, respectively. SN - 1420-3049 UR - https://www.unboundmedicine.com/medline/citation/33803330/Chemical_Composition_Antioxidant_and_Cytoprotective_Potentials_of_Carica_papaya_Leaf_Extracts:_A_Comparison_of_Supercritical_Fluid_and_Conventional_Extraction_Methods_ L2 - https://www.mdpi.com/resolver?pii=molecules26051489 DB - PRIME DP - Unbound Medicine ER -