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Probiotic Lactobacillus sp. Strains Inhibit Growth, Adhesion, Biofilm Formation, and Gene Expression of Bacterial Vaginosis-Inducing Gardnerella vaginalis.
Microorganisms. 2021 Mar 31; 9(4)M

Abstract

Gardnerella vaginalis contributes significantly to bacterial vaginosis, which causes an ecological imbalance in vaginal microbiota and presents with the depletion of Lactobacillus sp. Lactobacillus supplementation was reported to be an approach to treat bacterial vaginosis. We investigated the applicability of three Lactobacillus sp. strains (Lactobacillus delbrueckii DM8909, Lactiplantibacillus plantarum ATCC14917, and Lactiplantibacillus plantarum ZX27) based on their probiotic abilities in vitro. The three candidate Lactobacillus sp. strains for bacterial vaginosis therapy showed distinct properties in auto-aggregation ability, hydrophobicity, adhesion to cervical epithelial cells, and survivability in 0.01% hydrogen peroxide. Lpb. plantarum ZX27 showed a higher yield in producing short-chain fatty acids and lactic acid among the three candidate strains, and all three Lactobacillus sp. strains inhibited the growth and adhesion of G. vaginalis. Furthermore, we discovered that the culture supernatant of Lactobacillus sp. exhibited anti-biofilm activity against G. vaginalis. In particular, the Lpb. plantarum ZX27 supernatant treatment decreased the expression of genes related to virulence factors, adhesion, biofilm formation, metabolism, and antimicrobial resistance in biofilm-forming cells and suspended cells. Moreover, Lactobacillus sp. decreased the upregulated expression of interleukin-8 in HeLa cells induced by G. vaginalis or hydrogen peroxide. These results demonstrate the efficacy of Lactobacillus sp. application for treating bacterial vaginosis by limiting the growth, adhesion, biofilm formation, and virulence properties of G. vaginalis.

Authors+Show Affiliations

College of Pharmacy, Shanghai Jiaotong University, Shanghai 200240, China. State Key Laboratory of Microbial Metabolism, Shanghai Jiao Tong University, Shanghai 200240, China. Shanghai Institute of Pharmaceutical Industry, China State Institute of Pharmaceutical Industry, Shanghai 201203, China.College of Pharmacy, Shanghai Jiaotong University, Shanghai 200240, China.College of Pharmacy, Shanghai Jiaotong University, Shanghai 200240, China.College of Pharmacy, Shanghai Jiaotong University, Shanghai 200240, China.College of Pharmacy, Shanghai Jiaotong University, Shanghai 200240, China.College of Pharmacy, Shanghai Jiaotong University, Shanghai 200240, China.College of Pharmacy, Shanghai Jiaotong University, Shanghai 200240, China.College of Pharmacy, Shanghai Jiaotong University, Shanghai 200240, China.College of Pharmacy, Shanghai Jiaotong University, Shanghai 200240, China. State Key Laboratory of Microbial Metabolism, Shanghai Jiao Tong University, Shanghai 200240, China. Shanghai Institute of Pharmaceutical Industry, China State Institute of Pharmaceutical Industry, Shanghai 201203, China.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

33807455

Citation

Qian, Zhixiang, et al. "Probiotic Lactobacillus Sp. Strains Inhibit Growth, Adhesion, Biofilm Formation, and Gene Expression of Bacterial Vaginosis-Inducing Gardnerella Vaginalis." Microorganisms, vol. 9, no. 4, 2021.
Qian Z, Zhu H, Zhao D, et al. Probiotic Lactobacillus sp. Strains Inhibit Growth, Adhesion, Biofilm Formation, and Gene Expression of Bacterial Vaginosis-Inducing Gardnerella vaginalis. Microorganisms. 2021;9(4).
Qian, Z., Zhu, H., Zhao, D., Yang, P., Gao, F., Lu, C., Yin, Y., Kan, S., & Chen, D. (2021). Probiotic Lactobacillus sp. Strains Inhibit Growth, Adhesion, Biofilm Formation, and Gene Expression of Bacterial Vaginosis-Inducing Gardnerella vaginalis. Microorganisms, 9(4). https://doi.org/10.3390/microorganisms9040728
Qian Z, et al. Probiotic Lactobacillus Sp. Strains Inhibit Growth, Adhesion, Biofilm Formation, and Gene Expression of Bacterial Vaginosis-Inducing Gardnerella Vaginalis. Microorganisms. 2021 Mar 31;9(4) PubMed PMID: 33807455.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Probiotic Lactobacillus sp. Strains Inhibit Growth, Adhesion, Biofilm Formation, and Gene Expression of Bacterial Vaginosis-Inducing Gardnerella vaginalis. AU - Qian,Zhixiang, AU - Zhu,Hui, AU - Zhao,Dan, AU - Yang,Ping, AU - Gao,Fei, AU - Lu,Chunyi, AU - Yin,Yu, AU - Kan,Shidong, AU - Chen,Daijie, Y1 - 2021/03/31/ PY - 2021/02/19/received PY - 2021/03/26/revised PY - 2021/03/28/accepted PY - 2021/4/3/entrez PY - 2021/4/4/pubmed PY - 2021/4/4/medline KW - Gardnerella vaginalis KW - Lactobacillus KW - anti-inflammatory KW - biofilm KW - gene expression JF - Microorganisms JO - Microorganisms VL - 9 IS - 4 N2 - Gardnerella vaginalis contributes significantly to bacterial vaginosis, which causes an ecological imbalance in vaginal microbiota and presents with the depletion of Lactobacillus sp. Lactobacillus supplementation was reported to be an approach to treat bacterial vaginosis. We investigated the applicability of three Lactobacillus sp. strains (Lactobacillus delbrueckii DM8909, Lactiplantibacillus plantarum ATCC14917, and Lactiplantibacillus plantarum ZX27) based on their probiotic abilities in vitro. The three candidate Lactobacillus sp. strains for bacterial vaginosis therapy showed distinct properties in auto-aggregation ability, hydrophobicity, adhesion to cervical epithelial cells, and survivability in 0.01% hydrogen peroxide. Lpb. plantarum ZX27 showed a higher yield in producing short-chain fatty acids and lactic acid among the three candidate strains, and all three Lactobacillus sp. strains inhibited the growth and adhesion of G. vaginalis. Furthermore, we discovered that the culture supernatant of Lactobacillus sp. exhibited anti-biofilm activity against G. vaginalis. In particular, the Lpb. plantarum ZX27 supernatant treatment decreased the expression of genes related to virulence factors, adhesion, biofilm formation, metabolism, and antimicrobial resistance in biofilm-forming cells and suspended cells. Moreover, Lactobacillus sp. decreased the upregulated expression of interleukin-8 in HeLa cells induced by G. vaginalis or hydrogen peroxide. These results demonstrate the efficacy of Lactobacillus sp. application for treating bacterial vaginosis by limiting the growth, adhesion, biofilm formation, and virulence properties of G. vaginalis. SN - 2076-2607 UR - https://www.unboundmedicine.com/medline/citation/33807455/Probiotic_Lactobacillus_sp__Strains_Inhibit_Growth_Adhesion_Biofilm_Formation_and_Gene_Expression_of_Bacterial_Vaginosis_Inducing_Gardnerella_vaginalis_ L2 - https://www.mdpi.com/resolver?pii=microorganisms9040728 DB - PRIME DP - Unbound Medicine ER -
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