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The carriers of native vitamin B12 in normal human serum.
Clin Sci Mol Med. 1977 Nov; 53(5):453-7.CS

Abstract

1. After fractionation of the vitamin B12-binding proteins of ten normal sera the components containing transcobalamin II and R-type binders of vitamin B12 respectively were studied for endogenous vitamin B12 content by two distinct systems of vitamin B12 assay. 2. The measurements of total serum vitamin B12 by either bioassay with Euglena gracilis or a radioisotope dilution assay agreed closely. 3. The native vitamin B12 carried by transcobalamin II was higher as measured by bioassay than by isotope dilution assay. 4. The presence of the transcobalamin II fraction of human serum altered the key reaction between the binding reagent of the isotope dilution assay, so that this assay failed to measure vitamin B12 quantitatively. 5. Probably, the mean fraction of plasma vitamin B12 carried by transcobalamin II is in the range 20--30%.

Authors

No affiliation info available

Pub Type(s)

Journal Article
Research Support, U.S. Gov't, Non-P.H.S.

Language

eng

PubMed ID

338237

Citation

Hall, C A.. "The Carriers of Native Vitamin B12 in Normal Human Serum." Clinical Science and Molecular Medicine, vol. 53, no. 5, 1977, pp. 453-7.
Hall CA. The carriers of native vitamin B12 in normal human serum. Clin Sci Mol Med. 1977;53(5):453-7.
Hall, C. A. (1977). The carriers of native vitamin B12 in normal human serum. Clinical Science and Molecular Medicine, 53(5), 453-7.
Hall CA. The Carriers of Native Vitamin B12 in Normal Human Serum. Clin Sci Mol Med. 1977;53(5):453-7. PubMed PMID: 338237.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The carriers of native vitamin B12 in normal human serum. A1 - Hall,C A, PY - 1977/11/1/pubmed PY - 1977/11/1/medline PY - 1977/11/1/entrez SP - 453 EP - 7 JF - Clinical science and molecular medicine JO - Clin Sci Mol Med VL - 53 IS - 5 N2 - 1. After fractionation of the vitamin B12-binding proteins of ten normal sera the components containing transcobalamin II and R-type binders of vitamin B12 respectively were studied for endogenous vitamin B12 content by two distinct systems of vitamin B12 assay. 2. The measurements of total serum vitamin B12 by either bioassay with Euglena gracilis or a radioisotope dilution assay agreed closely. 3. The native vitamin B12 carried by transcobalamin II was higher as measured by bioassay than by isotope dilution assay. 4. The presence of the transcobalamin II fraction of human serum altered the key reaction between the binding reagent of the isotope dilution assay, so that this assay failed to measure vitamin B12 quantitatively. 5. Probably, the mean fraction of plasma vitamin B12 carried by transcobalamin II is in the range 20--30%. SN - 0301-0538 UR - https://www.unboundmedicine.com/medline/citation/338237/The_carriers_of_native_vitamin_B12_in_normal_human_serum_ L2 - https://portlandpress.com/clinsci/article-lookup/doi/10.1042/cs0530453 DB - PRIME DP - Unbound Medicine ER -