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FISH landmarks reflecting meiotic recombination and structural alterations of chromosomes in wheat (Triticum aestivum L.).
BMC Plant Biol. 2021 Apr 06; 21(1):167.BP

Abstract

BACKGROUND

DNA sequence composition affects meiotic recombination. However, the correlation between tandem repeat composition and meiotic recombination in common wheat (Triticum aestivum L.) is unclear.

RESULTS

Non-denaturing fluorescent in situ hybridization (ND-FISH) with oligonucleotide (oligo) probes derived from tandem repeats and single-copy FISH were used to investigate recombination in three kinds of the long arm of wheat 5A chromosome (5AL). 5AL535-18/275 arm carries the tandem repeats pTa-535, Oligo-18, and pTa-275, 5AL119.2-18/275 arm carries the tandem repeats pSc119.2, Oligo-18 and pTa-275, and 5AL119.2 arm carries the tandem repeats pSc119.2. In the progeny of 5AL535-18/275 × 5AL119.2, double recombination occurred between pSc119.2 and pTa-535 clusters (119-535 interval), and between pTa-535 and Oligo-18/pTa-275 clusters (535-18 interval). The recombination rate in the 119-535 interval in the progeny of 5AL535-18/275 × 5AL119.2-18/275 was higher than that in the progeny of 5AL535-18/275 × 5AL119.2. Recombination in the 119-535 interval produced 5AL119 + 535 segments with pTa-535 and pSc119.2 tandem repeats and 5ALNo segments without these repeats. The 5AL119 + 535 and 5ALNo segments were localized between the signal sites of the single-copy probes SC5A-479 and SC5A-527. The segment between SC5A-479 and SC5A-527 in the metaphase 5ALNo was significantly longer than that in the metaphase 5AL119 + 535.

CONCLUSION

The structural variations caused by tandem repeats might be one of the factors affecting meiotic recombination in wheat. Meiotic recombination aggregated two kinds of tandemly repeated clusters into the same chromosome, making the metaphase chromosome more condensed. To conclude, our study provides a robust tool to measure meiotic recombination and select parents for wheat breeding programs.

Links

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Authors+Show Affiliations

Zou Y
College of Agronomy, Sichuan Agricultural University, Wenjiang, 611130, Sichuan, China. Institute of Ecological Agriculture, Sichuan Agricultural University, Wenjiang, 611130, Sichuan, China. Provincial Key Laboratory for Plant Genetics and Breeding, Wenjiang, 611130, Sichuan, China.
Wan L
College of Agronomy, Sichuan Agricultural University, Wenjiang, 611130, Sichuan, China. Provincial Key Laboratory for Plant Genetics and Breeding, Wenjiang, 611130, Sichuan, China.
Luo J
College of Agronomy, Sichuan Agricultural University, Wenjiang, 611130, Sichuan, China. Institute of Ecological Agriculture, Sichuan Agricultural University, Wenjiang, 611130, Sichuan, China. Provincial Key Laboratory for Plant Genetics and Breeding, Wenjiang, 611130, Sichuan, China.
Tang Z
College of Agronomy, Sichuan Agricultural University, Wenjiang, 611130, Sichuan, China. zxtang@sicau.edu.cn. Institute of Ecological Agriculture, Sichuan Agricultural University, Wenjiang, 611130, Sichuan, China. zxtang@sicau.edu.cn. Provincial Key Laboratory for Plant Genetics and Breeding, Wenjiang, 611130, Sichuan, China. zxtang@sicau.edu.cn.
Fu S
College of Agronomy, Sichuan Agricultural University, Wenjiang, 611130, Sichuan, China. fushulan@sicau.edu.cn. Provincial Key Laboratory for Plant Genetics and Breeding, Wenjiang, 611130, Sichuan, China. fushulan@sicau.edu.cn.

MeSH

Chromosomes, PlantHomologous RecombinationIn Situ Hybridization, FluorescenceMeiosisOligonucleotide ProbesTriticum

Pub Type(s)

Journal Article

Language

eng

PubMed ID

33823797

Citation

Zou, Yang, et al. "FISH Landmarks Reflecting Meiotic Recombination and Structural Alterations of Chromosomes in Wheat (Triticum Aestivum L.)." BMC Plant Biology, vol. 21, no. 1, 2021, p. 167.
Zou Y, Wan L, Luo J, et al. FISH landmarks reflecting meiotic recombination and structural alterations of chromosomes in wheat (Triticum aestivum L.). BMC Plant Biol. 2021;21(1):167.
Zou, Y., Wan, L., Luo, J., Tang, Z., & Fu, S. (2021). FISH landmarks reflecting meiotic recombination and structural alterations of chromosomes in wheat (Triticum aestivum L.). BMC Plant Biology, 21(1), 167. https://doi.org/10.1186/s12870-021-02947-1
Zou Y, et al. FISH Landmarks Reflecting Meiotic Recombination and Structural Alterations of Chromosomes in Wheat (Triticum Aestivum L.). BMC Plant Biol. 2021 Apr 6;21(1):167. PubMed PMID: 33823797.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - FISH landmarks reflecting meiotic recombination and structural alterations of chromosomes in wheat (Triticum aestivum L.). AU - Zou,Yang, AU - Wan,Linrong, AU - Luo,Jie, AU - Tang,Zongxiang, AU - Fu,Shulan, Y1 - 2021/04/06/ PY - 2020/11/09/received PY - 2021/03/29/accepted PY - 2021/4/7/entrez PY - 2021/4/8/pubmed PY - 2021/4/24/medline KW - Chromosome condensation KW - Meiotic recombination KW - Tandem repeats KW - Wheat SP - 167 EP - 167 JF - BMC plant biology JO - BMC Plant Biol VL - 21 IS - 1 N2 - BACKGROUND: DNA sequence composition affects meiotic recombination. However, the correlation between tandem repeat composition and meiotic recombination in common wheat (Triticum aestivum L.) is unclear. RESULTS: Non-denaturing fluorescent in situ hybridization (ND-FISH) with oligonucleotide (oligo) probes derived from tandem repeats and single-copy FISH were used to investigate recombination in three kinds of the long arm of wheat 5A chromosome (5AL). 5AL535-18/275 arm carries the tandem repeats pTa-535, Oligo-18, and pTa-275, 5AL119.2-18/275 arm carries the tandem repeats pSc119.2, Oligo-18 and pTa-275, and 5AL119.2 arm carries the tandem repeats pSc119.2. In the progeny of 5AL535-18/275 × 5AL119.2, double recombination occurred between pSc119.2 and pTa-535 clusters (119-535 interval), and between pTa-535 and Oligo-18/pTa-275 clusters (535-18 interval). The recombination rate in the 119-535 interval in the progeny of 5AL535-18/275 × 5AL119.2-18/275 was higher than that in the progeny of 5AL535-18/275 × 5AL119.2. Recombination in the 119-535 interval produced 5AL119 + 535 segments with pTa-535 and pSc119.2 tandem repeats and 5ALNo segments without these repeats. The 5AL119 + 535 and 5ALNo segments were localized between the signal sites of the single-copy probes SC5A-479 and SC5A-527. The segment between SC5A-479 and SC5A-527 in the metaphase 5ALNo was significantly longer than that in the metaphase 5AL119 + 535. CONCLUSION: The structural variations caused by tandem repeats might be one of the factors affecting meiotic recombination in wheat. Meiotic recombination aggregated two kinds of tandemly repeated clusters into the same chromosome, making the metaphase chromosome more condensed. To conclude, our study provides a robust tool to measure meiotic recombination and select parents for wheat breeding programs. SN - 1471-2229 UR - https://www.unboundmedicine.com/medline/citation/33823797/FISH_landmarks_reflecting_meiotic_recombination_and_structural_alterations_of_chromosomes_in_wheat__Triticum_aestivum_L___ DB - PRIME DP - Unbound Medicine ER -