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Deoxygenation-induced cation fluxes in sickle cells: relationship between net potassium efflux and net sodium influx.
Blood Cells 1988; 13(3):339-58BC

Abstract

Deoxygenation-induced cation fluxes in sickle cells were studied by measuring net cation movements in ouabain-treated cells. These deoxy cation fluxes were highly dependent on pH, showing inhibition at pH less than 7 and greater than 8 and a maximum at 7.4-7.5. Activation occurred at oxygen tensions around 40-50 torr and fluxes rose sharply as PO2 fell lower. Deoxy K efflux paralleled deoxy Na influx at pH values between 7 and 8, and at all oxygen tensions. Sickle cells were separated by density on Percol-Stractan gradients. Dense cells had lower deoxy cation fluxes of both Na and K than did lighter cell fractions, but in none of the fractionated populations did deoxy K efflux exceed deoxy Na influx. These data demonstrate that deoxy cation fluxes are activated at physiological pH and oxygen tensions and that there are no conditions of pH and PO2 and no cell populations in which cation fluxes induced by deoxygenation contribute directly to net cation loss in sickle cells. Chloride replacement (with nitrate) did not alter deoxy cation fluxes, and deoxy K efflux did not require the presence of external Na (tetramethylammonium replacement). Thus, deoxy cation fluxes do not have the characteristics of a cation-chloride cotransport or cation countertransport system.

Authors+Show Affiliations

Department of Pediatrics, University of Alabama School of Medicine, Birmingham.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

3382745

Citation

Joiner, C H., et al. "Deoxygenation-induced Cation Fluxes in Sickle Cells: Relationship Between Net Potassium Efflux and Net Sodium Influx." Blood Cells, vol. 13, no. 3, 1988, pp. 339-58.
Joiner CH, Dew A, Ge DL. Deoxygenation-induced cation fluxes in sickle cells: relationship between net potassium efflux and net sodium influx. Blood Cells. 1988;13(3):339-58.
Joiner, C. H., Dew, A., & Ge, D. L. (1988). Deoxygenation-induced cation fluxes in sickle cells: relationship between net potassium efflux and net sodium influx. Blood Cells, 13(3), pp. 339-58.
Joiner CH, Dew A, Ge DL. Deoxygenation-induced Cation Fluxes in Sickle Cells: Relationship Between Net Potassium Efflux and Net Sodium Influx. Blood Cells. 1988;13(3):339-58. PubMed PMID: 3382745.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Deoxygenation-induced cation fluxes in sickle cells: relationship between net potassium efflux and net sodium influx. AU - Joiner,C H, AU - Dew,A, AU - Ge,D L, PY - 1988/1/1/pubmed PY - 1988/1/1/medline PY - 1988/1/1/entrez SP - 339 EP - 58 JF - Blood cells JO - Blood Cells VL - 13 IS - 3 N2 - Deoxygenation-induced cation fluxes in sickle cells were studied by measuring net cation movements in ouabain-treated cells. These deoxy cation fluxes were highly dependent on pH, showing inhibition at pH less than 7 and greater than 8 and a maximum at 7.4-7.5. Activation occurred at oxygen tensions around 40-50 torr and fluxes rose sharply as PO2 fell lower. Deoxy K efflux paralleled deoxy Na influx at pH values between 7 and 8, and at all oxygen tensions. Sickle cells were separated by density on Percol-Stractan gradients. Dense cells had lower deoxy cation fluxes of both Na and K than did lighter cell fractions, but in none of the fractionated populations did deoxy K efflux exceed deoxy Na influx. These data demonstrate that deoxy cation fluxes are activated at physiological pH and oxygen tensions and that there are no conditions of pH and PO2 and no cell populations in which cation fluxes induced by deoxygenation contribute directly to net cation loss in sickle cells. Chloride replacement (with nitrate) did not alter deoxy cation fluxes, and deoxy K efflux did not require the presence of external Na (tetramethylammonium replacement). Thus, deoxy cation fluxes do not have the characteristics of a cation-chloride cotransport or cation countertransport system. SN - 0340-4684 UR - https://www.unboundmedicine.com/medline/citation/3382745/Deoxygenation_induced_cation_fluxes_in_sickle_cells:_relationship_between_net_potassium_efflux_and_net_sodium_influx_ L2 - https://medlineplus.gov/potassium.html DB - PRIME DP - Unbound Medicine ER -