TY - JOUR T1 - Integrated miRNA-Seq and mRNA-Seq Study to Identify miRNAs Associated With Alzheimer's Disease Using Post-mortem Brain Tissue Samples. AU - Li,Qingqin S, AU - Cai,Diana, Y1 - 2021/03/23/ PY - 2020/10/24/received PY - 2021/02/23/accepted PY - 2021/4/9/entrez PY - 2021/4/10/pubmed PY - 2021/4/10/medline KW - Alzheimer’s disease KW - differentially express genes (DEGs) KW - hsa-miR-132 KW - hsa-miR-212 KW - mRNA-seq KW - miRNA-seq SP - 620899 EP - 620899 JF - Frontiers in neuroscience JO - Front Neurosci VL - 15 N2 - Alzheimer's disease (AD), the leading form of dementia, is associated with abnormal tau and β-amyloid accumulation in the brain. We conducted a miRNA-seq study to identify miRNAs associated with AD in the post-mortem brain from the inferior frontal gyrus (IFG, n = 69) and superior temporal gyrus (STG, n = 81). Four and 64 miRNAs were differentially expressed (adjusted p-value < 0.05) in AD compared to cognitively normal controls in the IFG and STG, respectively. We observed down-regulation of several miRNAs that have previously been implicated in AD, including hsa-miR-212-5p and hsa-miR-132-5p, in AD samples across both brain regions, and up-regulation of hsa-miR-146a-5p, hsa-miR-501-3p, hsa-miR-34a-5p, and hsa-miR-454-3p in the STG. The differentially expressed miRNAs were previously implicated in the formation of amyloid-β plaques, the dysregulation of tau, and inflammation. We have also observed differential expressions for dozens of other miRNAs in the STG, including hsa-miR-4446-3p, that have not been described previously. Putative targets of these miRNAs (adjusted p-value < 0.1) were found to be involved in Wnt signaling pathway, MAPK family signaling cascades, sphingosine 1-phosphate (S1P) pathway, adaptive immune system, innate immune system, and neurogenesis. Our results support the finding of dysregulated miRNAs previously implicated in AD and propose additional miRNAs that appear to be dysregulated in AD for experimental follow-up. SN - 1662-4548 UR - https://www.unboundmedicine.com/medline/citation/33833661/Integrated_miRNA_Seq_and_mRNA_Seq_Study_to_Identify_miRNAs_Associated_With_Alzheimer's_Disease_Using_Post_mortem_Brain_Tissue_Samples_ DB - PRIME DP - Unbound Medicine ER -