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Dust exposure and pulmonary inflammation in Standardbred racehorses fed dry hay or haylage: A pilot study.
Vet J. 2021 May; 271:105654.VJ

Abstract

Respirable dust exposure is linked to airway inflammation in racehorses. Feeding haylage may reduce dust exposure by 60-70%. The objective of this study was to compare dust exposure, airway cytology, and inflammatory cytokine concentrations between horses fed haylage or hay over 6 weeks while in training. Seven healthy Standardbred horses were randomly assigned to be fed alfalfa hay (n = 3) or grass-alfalfa mix haylage (n = 4) for six weeks while training on a treadmill. Dust exposure was measured gravimetrically at the breathing zone. Endotoxin and β-glucan concentrations in respirable dust were measured. Bronchoalveolar lavage fluid (BALF) cytology was determined at baseline and after 2, 4, and 6 weeks. Cytokine concentrations (interferon-γ, tumor necrosis factor-α, and interleukin-4) were measured in BALF at baseline and week 6. The effect of forage on exposure, airway cytology and cytokines were evaluated using generalized linear mixed models. Respirable dust and β-glucan exposures were lower in horses fed haylage than hay (0.02 ± 0.001 mg/m3 vs. 0.06 ± 0.01 mg/m3; P = 0.03, and 69 ± 18 pg/m3 vs. 160 ± 21 pg/m3; P = 0.02, respectively). In horses eating haylage, BALF neutrophil proportion decreased between baseline (2.2 ± 0.5%), week 2 (0.8 ± 0.3%; P = 0.01) and week 6 (0.7 ± 0.2%; P = 0.03). By week 6, horses fed haylage had lower BALF neutrophilia than horses fed hay (4.0 ± 0.7 %; P = 0.0004). Interleukin-4 concentration in BALF was higher at week 6 (14.4 ± 4.6 pg/mL) in horses fed hay compared to baseline (2.9 ± 4.6 pg/mL; P = 0.007). In conclusion, feeding haylage instead of hay to horses in training can reduce exposure to respirable irritants and mitigate airway neutrophilia.

Authors+Show Affiliations

Department of Veterinary Clinical Sciences, College of Veterinary Medicine, Purdue University, 625 Harrison St., West Lafayette, IN 47907, USA.Department of Veterinary Clinical Sciences, College of Veterinary Medicine, Purdue University, 625 Harrison St., West Lafayette, IN 47907, USA.Department of Veterinary Clinical Sciences, College of Veterinary Medicine, Purdue University, 625 Harrison St., West Lafayette, IN 47907, USA. Electronic address: couetill@purdue.edu.Department of Veterinary Clinical Sciences, College of Veterinary Medicine, Purdue University, 625 Harrison St., West Lafayette, IN 47907, USA.Department of Veterinary Clinical Sciences, College of Veterinary Medicine, Purdue University, 625 Harrison St., West Lafayette, IN 47907, USA.Department of Veterinary Clinical Sciences, College of Veterinary Medicine, Purdue University, 625 Harrison St., West Lafayette, IN 47907, USA.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

33840486

Citation

Olave, C J., et al. "Dust Exposure and Pulmonary Inflammation in Standardbred Racehorses Fed Dry Hay or Haylage: a Pilot Study." Veterinary Journal (London, England : 1997), vol. 271, 2021, p. 105654.
Olave CJ, Ivester KM, Couetil LL, et al. Dust exposure and pulmonary inflammation in Standardbred racehorses fed dry hay or haylage: A pilot study. Vet J. 2021;271:105654.
Olave, C. J., Ivester, K. M., Couetil, L. L., Kritchevsky, J. E., Tinkler, S. H., & Mukhopadhyay, A. (2021). Dust exposure and pulmonary inflammation in Standardbred racehorses fed dry hay or haylage: A pilot study. Veterinary Journal (London, England : 1997), 271, 105654. https://doi.org/10.1016/j.tvjl.2021.105654
Olave CJ, et al. Dust Exposure and Pulmonary Inflammation in Standardbred Racehorses Fed Dry Hay or Haylage: a Pilot Study. Vet J. 2021;271:105654. PubMed PMID: 33840486.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Dust exposure and pulmonary inflammation in Standardbred racehorses fed dry hay or haylage: A pilot study. AU - Olave,C J, AU - Ivester,K M, AU - Couetil,L L, AU - Kritchevsky,J E, AU - Tinkler,S H, AU - Mukhopadhyay,A, Y1 - 2021/03/13/ PY - 2020/07/23/received PY - 2021/02/22/revised PY - 2021/03/09/accepted PY - 2021/4/12/entrez PY - 2021/4/13/pubmed PY - 2021/6/5/medline KW - Asthma KW - Beta-glucan exposure KW - Horse KW - Neutrophils KW - Respiratory tract SP - 105654 EP - 105654 JF - Veterinary journal (London, England : 1997) JO - Vet J VL - 271 N2 - Respirable dust exposure is linked to airway inflammation in racehorses. Feeding haylage may reduce dust exposure by 60-70%. The objective of this study was to compare dust exposure, airway cytology, and inflammatory cytokine concentrations between horses fed haylage or hay over 6 weeks while in training. Seven healthy Standardbred horses were randomly assigned to be fed alfalfa hay (n = 3) or grass-alfalfa mix haylage (n = 4) for six weeks while training on a treadmill. Dust exposure was measured gravimetrically at the breathing zone. Endotoxin and β-glucan concentrations in respirable dust were measured. Bronchoalveolar lavage fluid (BALF) cytology was determined at baseline and after 2, 4, and 6 weeks. Cytokine concentrations (interferon-γ, tumor necrosis factor-α, and interleukin-4) were measured in BALF at baseline and week 6. The effect of forage on exposure, airway cytology and cytokines were evaluated using generalized linear mixed models. Respirable dust and β-glucan exposures were lower in horses fed haylage than hay (0.02 ± 0.001 mg/m3 vs. 0.06 ± 0.01 mg/m3; P = 0.03, and 69 ± 18 pg/m3 vs. 160 ± 21 pg/m3; P = 0.02, respectively). In horses eating haylage, BALF neutrophil proportion decreased between baseline (2.2 ± 0.5%), week 2 (0.8 ± 0.3%; P = 0.01) and week 6 (0.7 ± 0.2%; P = 0.03). By week 6, horses fed haylage had lower BALF neutrophilia than horses fed hay (4.0 ± 0.7 %; P = 0.0004). Interleukin-4 concentration in BALF was higher at week 6 (14.4 ± 4.6 pg/mL) in horses fed hay compared to baseline (2.9 ± 4.6 pg/mL; P = 0.007). In conclusion, feeding haylage instead of hay to horses in training can reduce exposure to respirable irritants and mitigate airway neutrophilia. SN - 1532-2971 UR - https://www.unboundmedicine.com/medline/citation/33840486/Dust_exposure_and_pulmonary_inflammation_in_Standardbred_racehorses_fed_dry_hay_or_haylage:_A_pilot_study_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1090-0233(21)00049-6 DB - PRIME DP - Unbound Medicine ER -