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A SNP of HD-ZIP I transcription factor leads to distortion of trichome morphology in cucumber (Cucumis sativus L.).
BMC Plant Biol. 2021 Apr 16; 21(1):182.BP

Abstract

BACKGROUND

Trichomes are excellent model systems for the analysis of cell differentiation and play essential roles in plant protection. From cucumber inbred line 'WD1', we identified an EMS-induced trichome abnormally developing mutant, nps, which exhibited smaller, denser and no pyramid-shaped head trichomes.

RESULTS

Using F2 and BC1 populations constructed from a cross between nps and '9930', the genetic analysis showed that the nps trait is controlled by a single recessive nuclear gene. We identified CsNps by map-based cloning with 576 individuals of the F2 population generated from the cross of nps and inbred line '9930'. The CsNps was located at a 13.4-kb genomic region on chromosome 3, which region contains three predicted genes. Sequence analysis showed that only one single nucleotide mutation (C → T) between 9930 and nps was found in the second exon of Csa3G748220, a plant-specific class I HD-Zip gene. The result of allelism test also indicated that nps is a novel allelic mutant of Mict (Micro-trichome). Thus, nps was renamed mict-L130F. By comparing the transcriptome of mict-L130F vs WD1 and 06-2 (mict) vs 06-1 (wildtype, near-isogenic line of 06-2), several potential target genes that may be related to trichome development were identified.

CONCLUSIONS

Our results demonstrate that Mict-L130F is involved in the morphogenesis of trichomes. Map-based cloning of the Mict-L130F gene could promote the study of trichome development in cucumber.

Authors+Show Affiliations

School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai, 200240, China.School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai, 200240, China.School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai, 200240, China.School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai, 200240, China.School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai, 200240, China.School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai, 200240, China.School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai, 200240, China.School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai, 200240, China.School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai, 200240, China. State Key Laboratory of Vegetable Germplasm Innovation, Tianjin, 300384, China.School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai, 200240, China. jspan71@sjtu.edu.cn.School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai, 200240, China. wg770801@sjtu.edu.cn.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

33863289

Citation

Zhang, Leyu, et al. "A SNP of HD-ZIP I Transcription Factor Leads to Distortion of Trichome Morphology in Cucumber (Cucumis Sativus L.)." BMC Plant Biology, vol. 21, no. 1, 2021, p. 182.
Zhang L, Lv D, Pan J, et al. A SNP of HD-ZIP I transcription factor leads to distortion of trichome morphology in cucumber (Cucumis sativus L.). BMC Plant Biol. 2021;21(1):182.
Zhang, L., Lv, D., Pan, J., Zhang, K., Wen, H., Chen, Y., Du, H., He, H., Cai, R., Pan, J., & Wang, G. (2021). A SNP of HD-ZIP I transcription factor leads to distortion of trichome morphology in cucumber (Cucumis sativus L.). BMC Plant Biology, 21(1), 182. https://doi.org/10.1186/s12870-021-02955-1
Zhang L, et al. A SNP of HD-ZIP I Transcription Factor Leads to Distortion of Trichome Morphology in Cucumber (Cucumis Sativus L.). BMC Plant Biol. 2021 Apr 16;21(1):182. PubMed PMID: 33863289.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - A SNP of HD-ZIP I transcription factor leads to distortion of trichome morphology in cucumber (Cucumis sativus L.). AU - Zhang,Leyu, AU - Lv,Duo, AU - Pan,Jian, AU - Zhang,Keyan, AU - Wen,Haifan, AU - Chen,Yue, AU - Du,Hui, AU - He,Huanle, AU - Cai,Run, AU - Pan,Junsong, AU - Wang,Gang, Y1 - 2021/04/16/ PY - 2021/01/21/received PY - 2021/03/24/accepted PY - 2021/4/17/entrez PY - 2021/4/18/pubmed PY - 2021/5/25/medline KW - Cucumber KW - Map-based cloning KW - Transcriptome KW - Trichome SP - 182 EP - 182 JF - BMC plant biology JO - BMC Plant Biol VL - 21 IS - 1 N2 - BACKGROUND: Trichomes are excellent model systems for the analysis of cell differentiation and play essential roles in plant protection. From cucumber inbred line 'WD1', we identified an EMS-induced trichome abnormally developing mutant, nps, which exhibited smaller, denser and no pyramid-shaped head trichomes. RESULTS: Using F2 and BC1 populations constructed from a cross between nps and '9930', the genetic analysis showed that the nps trait is controlled by a single recessive nuclear gene. We identified CsNps by map-based cloning with 576 individuals of the F2 population generated from the cross of nps and inbred line '9930'. The CsNps was located at a 13.4-kb genomic region on chromosome 3, which region contains three predicted genes. Sequence analysis showed that only one single nucleotide mutation (C → T) between 9930 and nps was found in the second exon of Csa3G748220, a plant-specific class I HD-Zip gene. The result of allelism test also indicated that nps is a novel allelic mutant of Mict (Micro-trichome). Thus, nps was renamed mict-L130F. By comparing the transcriptome of mict-L130F vs WD1 and 06-2 (mict) vs 06-1 (wildtype, near-isogenic line of 06-2), several potential target genes that may be related to trichome development were identified. CONCLUSIONS: Our results demonstrate that Mict-L130F is involved in the morphogenesis of trichomes. Map-based cloning of the Mict-L130F gene could promote the study of trichome development in cucumber. SN - 1471-2229 UR - https://www.unboundmedicine.com/medline/citation/33863289/A_SNP_of_HD_ZIP_I_transcription_factor_leads_to_distortion_of_trichome_morphology_in_cucumber__Cucumis_sativus_L___ DB - PRIME DP - Unbound Medicine ER -